Specification
7-50x continuous zoom stereo microscope | XTL-205A | XTL-205B | XTL-204A | XTL-2048 | XTL-203A | XTL-203B | |
Zoom Body | Binocular head,45°decline,360°rotate | ● | ● | ● | |||
Trinocular head,45°decline,360*rotate | ● | ● | ● | ||||
Eyepiece | WF10X22mm | ● | ● | ● | ● | ● | ● |
Objective | zoom objective 0.7X—5X | ● | ● | ● | ● | ● | ● |
Zoom Rate | 0.1:07.1 | ● | ● | ● | ● | ● | ● |
Total Amplific- ation Rate | the standard configuration is 7X-50X | ● | ● | ● | ● | ● | ● |
Auxiliary Lens | 0.5X,0.7X,1X,1.5X,2X For Option | O | O | O | O | O | O |
WorkingDistance | 100mm | ● | ● | ● | ● | ● | ● |
Diopter | 55mm-75mm | ● | ● | ● | ● | ● | ● |
Focus arm | 76mm Diameter,32mm pole size | ● | ● | ● | ● | ● | ● |
Plate | Black/White plate,Glass plate for option | ● | ● | ● | ● | ● | ● |
Optional | Stand | Q | Q | ||||
Stand | BI3 base.column sunnort unner and ower light source 3 WLED | ● | ● | ||||
BL3-A base vertical arm bracket upper and lower light source 3 WLED | ● | ● | |||||
B3 base column support has no light source | ● | ● | |||||
External light source | LED illumination | Q | Q | Q | O | ![]() | |
Optiona | Photography,camera accessories CCD Coupler | o | o | o | o | o | o |
Optional | Display attachment Monitor | ![]() |
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Figure 1 / 4
Click for expanded view
Kit Specifications | |
Maximum Column Binding Capacity | 35 μg for RNA 10 μg for DNA 100 μg for protein |
Maximum Column Loading Volume | 650 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Size of DNA Purified | ≥ 30 kb |
Time to Complete 10 Purifications | 30 minutes |
Maximum Amount of Starting Material:Animal CellsAnimal TissuesBloodPlant / Fungi Tissues | 5 x 105 cells5 mg (for selected tissues)50 µL25 mg |
Average Yield* HeLa Cells (5 x 105 cells) HeLa Cells (5 x 105 cells) HeLa Cells (5 x 105 cells) | RNA: 7.5 μg DNA: 1-2 μg Protein: 70 μg |
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 47700 (50 preps) | Cat. 51600 (50 preps) | Cat. 51700 (96 preps) |
---|---|---|---|
Buffer SKP | 40 mL | 40 mL | – |
Lysis Buffer Q | – | – | 40 mL |
Wash Solution A | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL |
Elution Solution A | 6 mL | 6 mL | 20 mL |
Elution Buffer F | 15 mL | 15 mL | 2 x 15 mL |
Wash Solution C | 30 mL | 30 mL | 60 mL |
Binding Buffer A | 8 mL | 8 mL | 8 mL |
Elution Buffer C | 8 mL | 8 mL | 30 mL |
Protein Neutralizer | 4 mL | 4 mL | 4 mL |
Protein Loading Dye | 2 mL | 2 mL | 3 x 2 mL |
gDNA Purification Columns | 50 | – | – |
gDNA Purification Micro Columns | – | 50 | – |
gDNA Purification 96-Well Plate | – | – | 1 |
RNA/Protein Purification Columns | 50 | – | – |
RNA/Protein Purification Micro Columns | – | 50 | – |
RNA/Protein Purification 96-Well Plate | – | – | 1 |
Collection Tubes | 150 | 150 | – |
Collection Plate | – | – | 5 |
Elution Tubes (1.7 mL) | 150 | 150 | – |
Elution Plate | – | – | 3 |
Lysis Preparation Plate | – | – | 2 |
Adhesive Tape | – | – | 4 |
Product Insert | 1 | 1 | 1 |
Description
The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
Storage
4°C for 12 months
-20°C for 36 months
The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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