16×5ml Angle rotor 

Introduction

Discover our Modified Tryptone Soya Broth, designed for the enrichment and cultivation of a wide range of microorganisms, ensuring accurate and reliable microbiological testing.

Discover our Modified Tryptone Soya Broth, designed for the enrichment and cultivation of a wide range of microorganisms, ensuring accurate and reliable microbiological testing.

Introduction

Intended Use

Used for the selective isolation and culture of Listeria monocytogenes.

Principle and Interpretation

Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and growth factors，maintains balanced osmotic pressure; Listeria hydrolyzes aesculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.

Formulation

Ingredients	/liter
Columbia blood agar base	39.0 g
Aesculin	1.0 g
Ferric ammonium citrate	0.5 g
Lithium chloride	15.0 g
pH7.0±0.2 at 25°C

Preparation

Weigh 55.5 g of this product, add 1000 mL of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15 min. Cool to about 50℃, add 1 bottle of SR0500 supporting reagent A and 1 bottle of B per 100 mL, mix well and set aside.

Quality Control

The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:

Quality control strains	Growth
Listeria monocytogenes ATCC19115	Gray-green colonies with a black depression in the center and black surrounding
Enterococcus faecalis ATCC29212	–
Escherichia coli ATCC25922	–

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use Used for the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and……

K-GAMINE

SKU: 700004290

50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)

Content:	50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	N-Acetyl-D-Glucosamine, D-Glucosamine, D-Glucosamine sulphate
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 µg of glucosamine per assay
Limit of Detection:	1.33 mg/L
Reaction Time (min):	~ 8 min
Application examples:	Food supplements, food products and beverages.
Method recognition:	Novel method

The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products. This kit can also be used to measure N-Acetyl-D-Glucosamine and D-Glucosamine sulphate.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

See our complete list of monosaccharide and disaccharide assay kits.

Advantages

• Novel product with simple format 
• All reagents stable for > 2 years after preparation 
• All enzymes supplied as stable suspensions 
• Very rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products. This kit can also be used to measure N-Acetyl-D-Glucosamine and D-Glucosamine sulphate.