Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.
This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.
High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.
Figure 1 / 8
Click for expanded view
Kit Specifications | |
Sample Volume Range | 250 µL |
Size of RNA purified | All sizes, including small RNA (<200 nt) |
Minimum Elution Volume | 75 µL |
Maximum Elution Volume | 100 µL |
Time to complete 96 purifications | 30 minutes |
Average Yield | ≥ 1 µg **varies from sample to sample |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Kit Component | Cat. 69100 (50 preps) | Cat. 69300 (192 preps) |
---|---|---|
Lysis Buffer A | 30 mL | 100 mL |
Solution WN | 18 mL | 55 mL |
Wash Solution A | 18 mL | 2 x 38 mL |
Elution Solution A | 6 mL | 20 mL |
Mini Spin Columns | 50 | – |
Collection Tubes | 50 | – |
Elution Tubes (1.7 mL) | 50 | – |
96-Well Isolation Plate | – | 2 |
96-Well Collection Plate | – | 2 |
96-Well Elution Plate | – | 2 |
Adhesive Tape | – | 4 |
Product Insert | 1 | 1 |
Methyltetrazine-PEG4-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Methyltetrazine-PEG4-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
Specifications
Features | Specifications |
Main Functions | Recover DNA fragments >100bp from agarose gel(<0.3g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA |
Applications | PCR, NGS, chip analysis, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Agarose Gel, PCR products, enzymatic reaction solution |
Sample amount | Agarose Gel: <0.3g, PCR products: ≤50µl |
Recovery | 80% |
Elution volume | ≥30μl |
Time per run | ≤30 minutes |
The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
Kit Contents
Contents | D500101 | D500102 | D500103 |
Purification Times | 50 Preps | 500 Preps | 5000 Preps |
Buffer GDP | 30 ml | 250 ml | 3 x 900 ml |
MagPure Particles | 1.6 ml | 15 ml | 3 x 50 ml |
Buffer DW1 | 20 ml | 180 ml | 2 x 800 ml |
Elution Buffer(10mmTris, pH8.5) | 10 ml | 60 ml | 500 ml |
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map