This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
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Kit Specifications – Spin Column | |
Column Binding Capacity | 10 μg |
Size of DNA Purified | 100 – 15,000 bp |
Average Recovery | > 90% |
Average Primer Removal | > 90% |
Minimum Elution Volume | 30 μL |
Time to Complete 10 Purifications | 15 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 14400 (50 preps) | Cat. 45700 (250 preps) | Cat. 24800 (192 preps) |
---|---|---|---|
Binding Buffer C | 30 mL | 5 x 30 mL | 3 x 30 mL |
Wash Solution A | 12 mL | 2 x 20 mL | 2 x 38 mL |
Elution Buffer B | 8 mL | 2 x 30 mL | 2 x 15 mL |
Spin Columns | 50 | 250 | – |
Collection Tubes | 50 | 250 | – |
96-Well Plate | – | – | 2 |
Adhesive Tape | – | – | 4 |
96-Well Collection Plate | – | – | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | – |
96-Well Elution Plate | – | – | 2 |
Product Insert | 1 | 1 | 2 |
Name of Product
HIT – Heparin-Induced Thrombocytopenia Test (20)
Catalog Number
MQHIT 1
Short Info
Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum
This product is only available in Germany!
Method/Platform
lateral flow, immunoassay
Range/Assay Sensivity
Test Principle
IgG antibodies are resposible for the heparin induced thrombocytopenia.
Immobilized anti-human IgG on the membrane of the test unit binds patients IgG-antibodies which are previously captured by the PF4/polyanion-complex which is detected by intensely colored gold nanoparticles.
The presence of PF4/polyanion-complex becomes visible at a colored test line. The surplus of gold particles continues to migrate through the membrane and is captured at the control line by specific antibodies.
0 tests
Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
Features | Specifications |
Main FunctionsC | Co-isolation DNA and RNA from a single FFPE tissue sample |
Applications | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | FFPE slice, FFPE embedded tissue |
Sample amount | No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area. |
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. For DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA was finally eluted with low-salt buffer.
1.Use non-toxic dewaxing solution without contact with xylene
2.Obtain both DNA and RNA simultaneously from the same sample. Elute separately without affecting each other (Have the same steps and effects as top brand 80234, perfect substitute.)
Contents | IVD5116 |
Purification Times | 50 Preps |
HiPure DNA Micro Column | 50 |
HiPure RNA Mini Column I | 50 |
2ml Collection Tubes | 150 |
Proteinase K | 50 mg |
Protease Dissolve Buffer | 5 ml |
Buffer DPS | 60 ml |
Buffer FRL | 15 ml |
Buffer ATL | 15 ml |
Buffer RLC | 15 ml |
Buffer AL | 15 ml |
Buffer VHB | 44 ml |
Buffer RW2 | 25 ml |
RNase Free Water | 10 ml |
Buffer AE | 10 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
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