• The Acetylcholinesterase Assay Kit provides a convenient method for the detecting AChE activity and screening for inhibitors.
• The kit uses DTNB to quantify the thiolcholine produced from the hydrolysis of acetylthiolcholine by AChE.

Description

AchE Inhibitor Screening Kit (Colorimetric) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of AchE inhibitors.

ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Inhibition of the enzyme leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. AChE inhibition is an important target for the management of Alzheimer’s disease. In addition to Alzheimer’s disease, AChE inhibitors have been useful in the diagnosis or treatment of diseases such as glaucoma, myasthenia gravis, bladder distention, and more.

The Attogene Acetylcholinesterase Inhibitor Assay is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5′-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412nm, is proportionate to the enzyme activity in the sample.

The Acetylcholinesterase Assay Kit provides a convenient method for the detecting AChE activity and screening for inhibitors.
The kit uses DTNB to quantify the thiolcholine produced from the hydrolysis of acetylthiolcholine by AChE.

Permagen’s MSPU750R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot, and consumable labware inconsistencies.

Also available in a kit form which includes our MTP96 clear top plate for 0.2 mL PCR tubes and strips.   

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

MSP750R (magnet plate only)

MSP750RKT (Includes top plate kit for 0.2 mL strips and tubes)

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Beads are pulled to tube walls for easy removal
• Integrated cushion base allowing for accidental contact between tips and well bottoms
• Made in USA 
• For Research use onl

Compatibility

• Full – Skirt PCR plates
• Semi- Skirted PCR plates 
• Non- Skirted PCR plates
• 0.2 mL PCR Strips and tubes  (With kit)
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Minimum – 30 µL 

Maximum – 0.2 mL

Permagen’s MSPU750R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot, and consumable labware inconsistencies.

Introduction

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine.

This product is for research use only.

Video

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
Applications	qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (vacuum)
Sample type	Serum, plasma and other cell-free fluid samples
Sample amount	1-5ml
Elution volume	≥50μl
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	1mg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.

Advantages

• High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration – low elution volume, ensuring high nucleic acid concentration
• High purity – low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery – DNA can be recovered at thelevel of PG by silica gel column purification

Kit Contents

Contents	D318201C	D318202C
Purification Times	10	50
Buffer ACL	50 ml	250 ml
Buffer ACB*	60 ml	300 ml
Buffer DCW1*	4.4 ml	22 ml
Buffer DCW2*	5 ml	10 ml
Proteinase K	110 ml	540 mg
Protease Dissolve Buffer	10 ml	30 ml
Carrier RNA	110 μg	110 μg
Nuclease Free Water	10 ml	20 ml
HiPure CFDNA Mini Columns	10	50
2 ml Collection Tubes	20	100
Extender Tube	10	50
Vac-Connector	10	50

Storage and Stability

Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,