Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could simplify your site-specific analysis of such modifications. The 2′-O-Me sensitive DNA polymerase was engineered to catalyse DNA synthesis from both DNA and RNA and to quantify 2′-O-methylation of nucleotides site-specifically from RNA by real-time PCR. For further information refer to the original publication.
Detail
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could simplify your site-specific analysis of such modifications. The 2′-O-Me sensitive DNA polymerase was engineered to catalyse DNA synthesis from both DNA and RNA and to quantify 2′-O-methylation of nucleotides site-specifically from RNA by real-time PCR. For further information refer to the original publication.
Available upon request and for R&D use only – Contact Us
The 2′-O-Me sensitive DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.
The enzyme can also be used for real-time cycling, when adding a suitable dye.
Amine-PEG4-Amide-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt
Product Info
Document
Product Info
Amine-PEG4-Amide-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt is a crosslinker consisting of an amino group with three propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research use only.
Document
Amine-PEG4-Amide-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt is a crosslinker consisting of an amino group with three propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research use only.
[CK1000] Champion™ E. coli Transformation Kit, 200 Rxn
Product Info
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Product Info
Description
Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.
Features
Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation.
Fast and Easy– only few steps for preparation; suitable for time-saving transformation
High efficiency– up to 109 cfu/μg
Personalization– suitable for most E. coli strains
Kit Contents
Component
Volume
Champion™ CC Buffer
20 ml
SMO-Broth™
100 ml x 2
pUC19 Control Plasmid (10-4 μg/μl)
5 µl
Instruction Manual
1
Champion™ Competent Cell Preparation Card
1
Storage
4°C for 12 months
Document
Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.
