20 bp DNA Ladder on 3.6% of agarose gel, 8% and 12% of acrylamide gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
20 bp DNA Ladder on 3.6% of agarose gel, 8% and 12% of acrylamide gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Figure 1 / 2
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Kit Specifications | |
Maximum Binding Capacity Per Column | Up to 50 μg RNA |
Maximum Loading Volume Per Spin Column | 650 μL |
Size of RNA Purified | All sizes, including < 200 nt |
Time to Complete 10 Purifications | 25 minutes |
Maximum Amount of Starting Material | |
Liver Tissue | 20 mg |
Heart Tissue | 5 mg |
Kidney Tissue | 20 mg |
Brain Tissue | 25 mg |
Lung Tissue | 20 mg |
Spleen Tissue | 20 mg |
Whole Blood | 100 µL |
Hela Cells | 3 x 106 |
CHO | 3 x 106 |
Yeast | 1 x 108 cells |
E.coli | 1 x 109 CFU/mL |
Bacteria | 1 x 109 cells |
Plasma/Serum | 200 µL |
Fungi | 50 mg |
Plant Tissue | 50 mg |
Viruses | ≤100 µL of viral suspension |
Average Yield | |
HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 48300 (50 preps) | Cat. 48400 (100 preps) |
---|---|---|
Buffer RL | 40 mL | 2 x 40 mL |
Wash Solution A | 38 mL | 2 x 38 mL |
Elution Solution A | 6 mL | 2 x 6 mL |
RNA Purification Columns | 50 | 100 |
Genomic DNA Removal Columns | 50 | 100 |
Collection Tubes | 100 | 200 |
Elution Tubes (1.7 mL) | 50 | 100 |
Product Insert | 1 | 1 |
Portable 4000rpm TD4C Centrifuge Machine for Lab, PRP & PRF
Application:
Widely used in the laboratory, hospital, clinic and so on.
Product Features:
1. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.
2. Rang of speed from 0~4000rpm, smooth in operation, low noise and small vibration.
3. Micro-computer control system, digital display the RCF, time and speed.
4. Electric cover lock, compact design, super speed and imblance protection.
5. Small and portable, very convenient to use.
Technical Parameters:
Max. Speed | 4000rpm | Speed Accuracy | ±20rpm | |
Max.Volume | 8x20ml | Acc/Dec | 10 Kinds | |
Max. RCF | 1880xg | Power Supply | AC110V/220V 50HZ/60HZ | |
Timer | 0~99min | Noise | ≤55dBA | |
Dimension | 310x270x220mm | Net Weight | 6.5Kg | |
Warranty | 1 Year | Certifications | CE, ISO & Calibration report are available. |
Matched Rotors:
Order No. | Rotor Type | Max.Speed(rpm) | Max.Volume(ml) | Max.RCF(xg) |
4C-1 | Angle Rotor | 4000 | 8x15ml/20ml | 1800/1840 |
4C-2 | Angle Rotor | 4000 | 6x10ml/15ml/20ml | 1790 |
4C-3 | Angle Rotor | 4000 | 12x5ml | 1540 |
4C-4 | Angle Rotor | 4000 | 12x10ml/7ml | 1792 |
4C-5 | Angle Rotor | 4000 | 8x10ml vacuum tube | 1790 |
8x5ml vacuum tube adapter | 1790 | |||
8x7ml vacuum tube adapter | 1790 |