Introduction

Usages:
For isolating lactose-fermenting Gram-negative enteric bacilli.

Principle:
Peptones are sources of nitrogen and other nutrients. Lactose is a fermentable carbohydrate. When lactose is fermented, alocal pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts,bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Agar is the solidifying agent.

Formulation（per liter）:

How to use：
1.Suspend 50 g in 1 L of distilled or deionized water. Heat to boiling to dissolve completely. Autoclave at 121°C for 15 minutes.

2.Transfer 1 mL of Soybean–Casein Digest Broth to 100 mL of MacConkey Broth, and incubate at 42 to 44 for 24 to 48 hours. Subculture on a plate of MacConkey Agar at 30 to 35 deg.C for 18 to 72 hours.

Quality control：
          　　

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of three years.

Specifications: 250g/bottle

250g

Opentrons OT-2 Tips, 300µL. Optimized for volumes 5µL to 300µL Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.

Opentrons OT-2 Tips, 300µL. Optimized for volumes 5µL to 300µL Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.

Introduction

Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.

Details

Specifications

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The sample is homogenized and lysed in the lysate, and RNA is released into the lysate. The high concentration of guanidine isothiocyanate contained in the lysate denatured and inactivated endogenous or exogenous RNase, while RNA is protected from degradation. The lysate is centrifuged to remove insoluble impurities. After adding ethanol to adjust the binding conditions, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is removed without adsorption. The column is washed with buffer VHB to remove protein and other impurities, washed with buffer RW2 to remove salt. Finally the RNA is eluted by RNase free water. The eluted RNA can be directly used in RT-PCR, Northern blot, poly-A purification, in vitro translation, etc.

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Storage and Stability

Carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions

Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.