228×7ml Swing Buckets Rotor

Overview

Heavy duty heat sealing foil which is peelable; suitable for long term storage and transportation.

• Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective
• Our ThermASeal Heat Seal is a heavy duty laminate foil seal suitable for providing a very strong, but peelable seal
• The seal is compatible with polypropylene plates to provide a high degree of sample protection
• It demonstrates very good solvent resistance and can be used for very low temperature compound storage, in DMSO and organic solvents, and long term room temperature storage such that it is recommended as suitable for sample transportation
• The seal can be pierced only by using a blade
• The seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Heavy duty heat sealing foil which is peelable; suitable for long term storage and transportation.

DBCO-PEG8-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG8-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
• Higher intensity reference bands at 500 bp and 1000 bp
• 2686 bp (pUC19) reference band for easy clone identification

The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, double intensity reference bands at 500 and 1000 bp and an additional 2686 bp (pUC19) reference band for easy clone identification.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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CloneSizer 100 bp DNA Ladder (Cat# 11600) – 100 loads

Ladder Properties:

• Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
• Higher intensity reference bands at 500 bp and 1000 bp
• 2686 bp (pUC19) reference band for easy clone identification

Fragment	Size (bp)	Mass (ng)
1	2686	72
2	2000	53
3	1500	41
4	1200	42
5	1000	56
6	900	30
7	800	29
8	700	25
9	600	25
10	500	52
11	400	19
12	300	20
13	200	19
14	100	17

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.