These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.
ProteoSpin™ Urine Protein Concentration Micro Kit
Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.
ProteoSpin™ Urine Protein Concentration Midi Kit
The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.
ProteoSpin™ Urine Protein Concentration Maxi Kit
The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.
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Kit Specifications | |
Minimum Urine Volume Input | 1 mL |
Maximum Urine Volume Input | 5 mL |
Maximum Recovered Proteins (5 mL Urine) | 3 mg* |
Elution Volume | 200 – 1000 μL |
Time to Complete 10 Purifications | 30 minutes |
*Yield will depend on the health status of the donor.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Component | Cat. 17400 (25 preps) | Cat. 52300 (10 preps) | Cat. 21600 (4 preps) |
---|---|---|---|
Wash Solution C | 60 mL | 60 mL | 130 mL |
Binding Buffer A | 4 mL | 4 mL | 8 mL |
Elution Buffer C | 8 mL | 30 mL | 30 mL |
Protein Neutralizer | 4 mL | 4 mL | 4 mL |
Micro Spin Columns | 25 | – | – |
Midi Spin Columns (assembled with collection tubes) | – | 10 | – |
Maxi Spin Columns (assembled with collection tubes) | – | – | 4 |
Collection Tubes | 25 | – | – |
Elution Tubes (1.7 mL) | 25 | – | – |
Midi Elution Tubes | – | 10 | – |
Elution Tubes (50 mL) | – | – | 4 |
Product Insert | 1 | 1 | 1 |
This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.
Specifications
Features | Specifications |
Main Functions | Isolation circulating DNA from 1-6ml plasma, serum, body fluids |
Applications | qPCR, NGS, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Serum, plasma |
Sample amount | 1-6ml |
Elution volume | ≥50μl |
Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Contents | IVD5435 |
Purification Times | 50 |
MagPure Particles F | 14 ml |
Carrier RNA | 310 μg |
Proteinase K | 240 mg |
Protease Dissolve Buffer | 15 ml |
Buffer SDS | 15 ml |
Buffer MLK | 500 ml |
Buffer MAW1 | 250 ml |
Buffer MW2* | 50 ml |
Elution Buffer | 60 ml |
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance.Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.
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