Opentrons 4-in-1 Tube Rack set includes 2 base stands and 4 tube holder tops: 1.5ml, 2ml, 15ml + 50ml, 15ml, and 50ml. The tube holder tops snap securely on the base stand, which fits directly on the deck. The Tube Rack Set is not autoclavable.
Opentrons 4-in-1 Tube Rack set includes 2 base stands and 4 tube holder tops: 1.5ml, 2ml, 15ml + 50ml, 15ml, and 50ml. The tube holder tops snap securely on the base stand, which fits directly on the deck. The Tube Rack Set is not autoclavable.
| Clone | IHC053 |
| Source | Mouse Monoclonal |
| Positive Control | Colon Carcinoma |
| Dilution Range | 1:200 |
p53, also known as tumor protein 53 or TP53, is a tumor suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumors, including those of the breast, ovarian, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Serum, plasma, acellular samples |
| Sample amount | 300μl |
This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | R662801 | R662802 | R662803 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles N | 1.7 ml | 3.5 ml | 17 ml |
| Buffer CFL | 6 ml | 12 ml | 60 ml |
| Buffer CPL | 1.8 ml | 3.5 ml | 20 ml |
| Buffer MGW1* | 30 ml | 60 ml | 250 ml |
| Buffer MW2* | 12 ml | 25 ml | 100 ml |
| RNase Free Water | 10 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particle N should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.