

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.5g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA |
| Applications | PCR, NGS, labeling, ligation and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Agarose gel, PCR products, enzyme products |
| Sample amount | Agarose gel: ≤500mg |
| Recovery | ≥80% |
| Elution volume | ≥15μl |
| Time per run | ≤20 minutes(1-24 samples) |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 35µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D211102 | D211103 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer GDP | 120 ml | 250 ml |
| Buffer DW2 | 50 ml | 2 x 50 ml |
| Elution Buffer | 20 ml | 30 ml |
| HiPure DNA Mini Columns II | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Experiment Data
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
*Optical system: equipped with infinity plan achromatic stress-free metallographic objective lens,built-in polarizing ober-vation device
*Observation head device:The lens barrel can be rotated 360 degrees,and the interpupillary distance adjustment range is 50-75MM
*Eyepiece configuration:random eyepiece WF10X /22mm;
*Objective:Infinity plan achromatic strain-free metallographic objective;5X,10X,20X,50X
*Loading platform:Stage:Tripod XY composite mechanical moving stage; size 140X120MM, stroke 80X50MM, minimum reading value 0.1mm
Specification configuration | XTL-20A | Trinocular observation head,transmission single light source 5W LED ighting |
| XTL-20B | Trinocular observation head,transparent reflection double light source up and down 5W LED lighting | |
| Eyepiece | Wide-angle eyepiece WF10Xfield of view 22mm,optional WF16X,WF25X | |
| Converter | Quadruple Revolving converter | |
| Objective | Infinite field achromatic stress-free objective,5X,10X,20X,50X | |
| Condenser | N.A.1.25 Swinging Abbe condenser with variable light bar | |
| Stage | Double Layer Mechanical Stage,Size1140X120mn,Moving Range :80x50mm | |
| Focusing | Coaxial Coase &Fine adjustment,fine division 0.02mm | |
| Light source | LED 5V lighting,DC1272A power adapter,adjustable brightness | |
| Attachments | Dust bag,Type C power cord | |
XTL-20 series metallographic microscopes are equipped with infinity plan achromatic stress-free metallographic objective lens and Wide-angle eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
Model:
XTL-20
Brand:
Yingtai