Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details

Specifications

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – ≥80% DNA recovery
• General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method
• Great cost-effectiveness performance

Kit Contents

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Experiment Data

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

*Optical system: equipped with infinity plan achromatic stress-free metallographic objective lens,built-in polarizing ober-vation device

*Observation head device:The lens barrel can be rotated 360 degrees,and the interpupillary distance adjustment range is 50-75MM

*Eyepiece configuration:random eyepiece WF10X /22mm;

*Objective:Infinity  plan  achromatic  strain-free   metallographic  objective;5X,10X,20X,50X

*Loading  platform:Stage:Tripod  XY  composite   mechanical  moving  stage; size 140X120MM, stroke 80X50MM, minimum reading value 0.1mm

XTL-20 series metallographic microscopes are equipped with infinity plan achromatic stress-free metallographic objective lens and Wide-angle eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
Model:
XTL-20

Brand:
Yingtai