The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
Specifications
Features | Specifications |
Main Functions | Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture |
Applications | Cell transfection, animal injection, etc. |
Purification method | Maxi spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Conventional plasmid vector |
Sample amount | High copy plasmid vector: 100-150ml culture mediumLow copy plasmid vector: 150-200ml culture medium |
Yield | 200-1500μg |
Elution volume | ≥0.7ml |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 20ml |
Binding yield of column | 1mg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | P115602 | P115603 |
Purification Times | 10 Preps | 50 Preps |
RNase A | 30 mg | 150 mg |
Buffer P1 | 100 ml | 500 ml |
Buffer P2 | 100 ml | 500 ml |
Buffer LN3 | 50 ml | 250 ml |
Buffer PW1 | 33 ml | 180 ml |
Buffer PW2 | 20 ml | 100 ml |
Elution Buffer | 15 ml | 120 ml |
Buffer CL | 33 ml | 180 ml |
HiPure DNA Maxi Columns C | 10 | 50 |
Lysate Clear Midi Syringe | 10 | 50 |
50 ml Collection Tubes C | 20 | 100 |
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2–8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from FFPE tissue samples |
Applications | PCR, Southern Blot and viral DNA detection, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples |
Sample amount | <20mg |
Elution volume | >15µl |
Time per run | ≤20 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 100μg |
Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After decrosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.
Contents | IVD3126 |
Purification Times | 100 Preps |
HiPure DNA Mini Columns I | 100 |
2ml Collection Tubes | 100 |
Buffer DPS | 70 ml |
Buffer ATL | 30 ml |
Buffer AL | 30 ml |
Buffer GW1* | 44 ml |
Buffer GW2* | 20 ml |
Proteinase K | 50 mg |
Protease Dissolve Buffer | 6 ml |
Buffer AE | 20 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Thermal adapter for PCR plates, e.g., NEST 96 Well Plate 100 µL PCR Full Skirt. Compatible with the Opentrons Heater-Shaker Module.
Thermal adapter for PCR plates, e.g., NEST 96 Well Plate 100 µL PCR Full Skirt. Compatible with the Opentrons Heater-Shaker Module.
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