t-Boc-aminooxy-PEG2-propargyl is a click chemistry crosslinker. The propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-aminooxy-PEG2-propargyl is a click chemistry crosslinker. The propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells |
| Sample amount | 1-1.5ml whole blood, 0.5-1ml bone marrow |
| Yield | 1-30μg |
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.
Advantages
Kit Contents
| Contents | R661101 | R661102 | R661103 |
| Purification Times | 48 Preps | 96 Preps | 480 preps |
| 10 x RBC Lysis Buffer | 50 ml | 2 x 50 ml | 4 x 100 ml |
| Proteinase K | 12 mg | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 1.8 ml | 10 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 µl |
| DNase Buffer | 20 ml | 30 ml | 150 ml |
| MagPure Particles N | 1.2 ml | 2.5 ml | 11 ml |
| Buffer RTL | 30 ml | 60 ml | 300 ml |
| Buffer ALB2 | 40 ml | 60 ml | 300 ml |
| Buffer MW1* | 22 ml | 44 ml | 220 ml |
| Buffer MW2* | 20 ml | 50 ml | 2 x 100 ml |
| RNase Free Water | 10 ml | 20 ml | 60 ml |
Storage and Stability
DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
