Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.
Organophosphate compounds (OP) account for the largest class of rural and urban poisons in the world that are used to kill pests but can also be toxic to humans. OPs cause toxicity by means of blocking the acetylcholinesterase enzyme (AchE). The AChE-directed OPs react with a serine residue that is located at the catalytic site found within the AChE gorge. The OP targeted enzyme is no longer able to hydrolyze ACh, resulting in the buildup of ACh in the nerve synapse. This effect causes excessive excitation of the nerves, producing uncoordinated movements, tremors, paralysis and death. Both synthetic and natural(Guanitoxin) organophosphates are dangerous to humans — exposure can lead to visual, coordination, muscular, and neurological deficiencies, and in some cases even to death. In turn, exposure to OP is a significant public health concern which would significantly benefit from an improved detection platform.
Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples. For solid samples a simple sample preparation method is performed. The ability to detect Organophosphate is performed is simple and sensitive. The reaction uses a chromophore that can be detected by eye. In the presence of Organophosphate, the rate of chromophore production is reduced in a concentration dependent fashion. The higher the concentration of Organophosphate the less color is produced.
Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.
K-MRH2O2
SKU: 700004322
1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
Content: | 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Hydrogen Peroxide |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Absorbance, Fluorescence |
Wavelength (nm): | (Fluorescence) 530-560 (ex), ~ 590 (em) (Absorbance) 570 |
Signal Response: | Increase |
Linear Range: | (Fluorescence) 0.0034-0.17 µg of H2O2 per assay (0.1-5 µM in-assay) (Absorbance) 0.034-0.68 µg of H2O2 per assay (1-20 µM in-assay) |
Limit of Detection: | (Fluorescence) 0.0034 ug/mL (Absorbance) 0.25 ug/mL |
Reproducibility (%): | (Fluorescence) ~ 8% (Absorbance) ~ 8% |
Reaction Time (min): | (Fluorescence) 30 min (Absorbance) 30 min |
Application examples: | Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.). |
This product has been discontinued.
The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.
Display all assay test kits available.
Fluorometric/UV method for the determination of H2O2 content in a variety of samples.
Advantages
The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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