K-LMAL-116A
SKU: 700004311
Content: | (K-LMAL-58A) 58 assays (manual) / 580 assays (microplate) (K-LMAL-116A) 116 assays (manual) / 1160 assays (microplate) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | L-Malic Acid |
Assay Format: | Spectrophotometer, Microplate |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Increase |
Linear Range: | 0.5 to 30 µg of L-malic acid per assay |
Limit of Detection: | 0.25 mg/L |
Reaction Time (min): | ~ 3 min |
Application examples: | Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Methods based on this principle have been accepted by AOAC, EEC, EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN, NBN, ISO and MEBAK |
The K-LMAL-58A pack size has been discontinued (read more)
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Need other assay kits? View our full list of organic acid assay kits.
Advantages
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Specifications
Features | Specifications |
Main Functions | Extract viral RNA/DNA from 200μl plasma/serum samples |
Applications | RT-PCR,PCR,NGS |
Products | Viral DNA / RNA, body cell DNA / RNA |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Plasma, serum, effusion, urine, fecal suspension supernatant |
Sample amount | 200μl |
Elution volume | ≥30μl |
Time per run | |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
Kit Contents
Contents | IVD4175 |
Purification Times | 100 Preps |
HiPure Viral Column | 100 |
2ml Collection Tubes | 100 |
PK/Carrier RNA | 50 mg/310μg |
Protease Dissolve Buffer | 5 ml |
Buffer VLE | 42 ml |
Buffer CE | 60 ml |
RNase Free Water | 15 ml |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Figure 1 / 4
Click for expanded view
Kit Specifications | |
Maximum Column Binding Capacity | 50 μg for RNA 20 μg for DNA 200 μg for protein |
Maximum Column Loading Volume | 650 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Size of DNA Purified | ≥ 30 kb |
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues | 5 x 106 cells 25 mg (for selected tissues) 100 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg |
Time to Complete 10 Purifications | 30 minutes |
Average Yield:HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells) | 15 μg RNA8 μg DNA150 μg protein |
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 47700 (50 preps) | Cat. 51600 (50 preps) | Cat. 51700 (96 preps) |
---|---|---|---|
Buffer SKP | 40 mL | 40 mL | – |
Lysis Buffer Q | – | – | 40 mL |
Wash Solution A | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL |
Elution Solution A | 6 mL | 6 mL | 20 mL |
Elution Buffer F | 15 mL | 15 mL | 2 x 15 mL |
Wash Solution C | 30 mL | 30 mL | 60 mL |
Binding Buffer A | 8 mL | 8 mL | 8 mL |
Elution Buffer C | 8 mL | 8 mL | 30 mL |
Protein Neutralizer | 4 mL | 4 mL | 4 mL |
Protein Loading Dye | 2 mL | 2 mL | 3 x 2 mL |
gDNA Purification Columns | 50 | – | – |
gDNA Purification Micro Columns | – | 50 | – |
gDNA Purification 96-Well Plate | – | – | 1 |
RNA/Protein Purification Columns | 50 | – | – |
RNA/Protein Purification Micro Columns | – | 50 | – |
RNA/Protein Purification 96-Well Plate | – | – | 1 |
Collection Tubes | 150 | 150 | – |
Collection Plate | – | – | 5 |
Elution Tubes (1.7 mL) | 150 | 150 | – |
Elution Plate | – | – | 3 |
Lysis Preparation Plate | – | – | 2 |
Adhesive Tape | – | – | 4 |
Product Insert | 1 | 1 | 1 |
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