Norgen’s Vibrio cholerae TaqMan PCR Lyophilized Kit is designed for the detection of Vibrio cholerae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s Vibrio cholerae TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Vibrio cholerae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
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Component | Cat. TM38550L (100 preps) | Cat. TM38510L (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix (Lyo) | 4 x 350 μL | – |
Vibrio cholerae Primer & Probe Mix (Lyo) | 280 μL | 280 μL |
Vibrio cholerae Positive Control (Lyo) | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 3 x 1.25 mL | 1 x 1.25 mL |
Product Insert | 1 | 1 |
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
Features | Specifications |
Main Functions | Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate |
Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
Purification method | 96 well Bind Plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | PCR product, enzymatic reaction |
Sample amount | Appropriate |
Recovery | ≥80% |
Elution volume | ≥75μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
Contents | D212201 | D212202 | D212203 |
Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
Buffer GDP | 120 ml | 400 ml | 4 x 400 ml |
Buffer DW2 | 20 ml | 100 ml | 3 x 100 ml |
Elution Buffer | 20 ml | 20 ml | 30 ml |
HiPure DNA Plate | 1 | 4 | 20 |
2.2ml Collection Plate | 1 | 4 | 20 |
1.6 ml Collection Plate | 1 | 4 | 20 |
0.8 ml Collection Plate | 1 | 4 | 20 |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Tetra(3-methoxy-N-(PEG5-prop-2-ynyl)propanamide) Methane is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.
Tetra(3-methoxy-N-(PEG5-prop-2-ynyl)propanamide) Methane is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.
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