250ml Tube

Description

Goat Anti-Rabbit (GAR) Conjugated Colloidal Gold for Lateral Flow (1mL of 10OD)

Product image shows functional testing of Goat Anti Rabbit 40nm colloidal gold on a lateral flow test.

Our products are produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.

Goat Anti-Rabbit (GAR) Conjugated Colloidal Gold for Lateral Flow (1mL of 10OD)

Product image shows functional testing of Goat Anti Rabbit 40nm colloidal gold on a lateral flow test.

Our products are produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.

Overview

• Extract total RNA (including microRNA) from FFPE samples
• No phenol extraction step
• Includes DNase for optional on-column DNA removal
• Isolated RNA is of the highest quality and integrity
• Isolate a diversity of RNA species
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.

FFPE RNA Purification Kit (Spin Column)

Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

FFPE RNA Purification 96-Well Kit (High Throughput)

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.

Details

Supporting Data

Figure 1 / 5

Previous

Next

Click for expanded view

Kit Specifications
Maximum Column Binding Capacity	Up to 50 µg RNA
Maximum Loading Volume Per Spin Column	650 µL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	1-4 hours*
Maximum Amount of Starting Material	5 slices of < 20 µm thick paraffin slices 25 mg of unsectioned block
Average Yield	Variable due to age of paraffin blocks ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney

* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal

​

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.

Component	Cat. 25300 (50 preps)	Cat. 25400 (2 x 96 preps)
Digestion Buffer A	25 mL	2 x 25 mL
Buffer RL	30 mL	2 x 30 mL
Enzyme Incubation Buffer	6 mL	2 x 6 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 20 mL
Proteinase K	12 mg	2 x 20 mg
DNase I	1 vial	2 x 500μL
Micro Spin Columns	50	–
96-Well Incubation Plate	–	2
96-Well Plate	–	2
Adhesive Tape	–	8
Collection Tubes	50	–
96-Well Collection Plate	–	2
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Product Insert	1	1

Features: 

1.Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.

2.Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.

3.Automatic lid lock, LCD display, It can store 100 programs. super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.

4.Adapters are available by experiment requirements.

5.3 tiers protection steel cover, safe and reliable. 

6.IEC CE EMC certificate.

 TD5 Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×250ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	570×400×320mm
Net /Gross weight(Kg)	42KG /47KG
Certificates	CE,ISO & Calibration report are available

 Matched Rotors for TD5 

Order No	Rotor	Max	Volume(ml)	Max RCF(×g)
		Speed(r/min)
D5-1	Swing rotor(round	4000	4×250ml	2880
	cup)
D5-2	Swing buckets	4000	4×16×7ml	2860
D5-3		4000	4×16×5ml	2470
D5-4		4000	4×13×10ml	2830
D5-5		5000	4×1×50ml	4730
	Swing rotor
D5-6			4×1×100ml	4730
D5-7			4×2×100ml	3020
D5-8			4×2×50ml	3020
D5-9			4×4×15ml	3020
D5-10			4×6×15ml	3020
D5-11			4×4×10/7	2770
D5-12			4×4×5 vacuum tube	2360
		#######	adapters
D5-13			4×6×10/7	2770
D5-14			4×6×5 vacuum tube	2360
			adapters
D5-15			4×8×10/7	2770
D5-16			4×8×5 vacuum tube	2360
			adapters
D5-17			4×12×5 vacuum tube	2480
D5-18			4×12×7 vacuum tube	2760
D5-19			4×10×10 vacuum tube	2880
D5-20	Microplate rotor	4000	2×3×96 well	2310
D5-21	Fixed rotor	5000	6×15ml	2540
D5-22		5000	12×15ml	3080
D5-23		5000	24×15ml	3500
D5-24		5000	30×15ml	3830
D5-25		5000	4×50ml	2520
D5-26	Fixed rotor	5000	6×50ml	2850
D5-27		4000	12×50ml	2470
D5-28		5000	4×100ml	2630
D5-29		5000	6×100ml	3130

 Adapter for 250 round cup and square cup 

VolumeCup	5ml	7ml	10ml	15ml	50ml
250ml（Square）	9pcs/cup	9	9	4	1
250ml（Round）	7pcs/cup	7	7	4	1

  Rotor with adapter