DNA amplification made possible with a TwistAmp® Basic kit. Contains all the enzymes and reagents necessary for the amplification of DNA, the user needs only supply primers and template. Even PCR primers can work using TwistAmp® Basic. TwistAmp® Basic has been used for solid-phase, tailed primers, aptamers, electrochemistry and microarray applications. See manual for more information. Click to order oligonucleotides.
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Description
DNA amplification made possible with a TwistAmp® Basic kit. Contains all the enzymes and reagents necessary for the amplification of DNA, the user needs only supply primers and template. Even PCR primers can work using TwistAmp® Basic. TwistAmp® Basic has been used for solid-phase, tailed primers, aptamers, electrochemistry and microarray applications. See manual for more information. Click to order oligonucleotides.
Perfect for: End-point gel electrophoresis DNA detection, down-stream applications (e.g. sub-cloning)
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EXTRAClean Exosomal RNA Isolation Kit
Product Info
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Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Isolates all sizes of exosomal and extracellular vesicle RNA, including microRNA.
Bind and elute all RNA irrespective of size or GC content, without bias.
No phenol extractions.
No Proteinase K treatment.
No carrier RNA.
Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
Purify high-quality RNA in 15-20 minutes.
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.
The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Exosomes purified using Norgen’s Purification Kits
Size of RNA Purified
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Propargyl-PEG5-methane has a propargyl group which can react with azide compounds via copper catalyzed Click Chemistry reactions. The PEG units increase water-solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG5-methane has a propargyl group which can react with azide compounds via copper catalyzed Click Chemistry reactions. The PEG units increase water-solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG4 enables Click Chemistry reactions with azide compounds via copper catalyzed azide-alkyne Click Chemistry. The PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Bis-propargyl-PEG4 enables Click Chemistry reactions with azide compounds via copper catalyzed azide-alkyne Click Chemistry. The PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
