DNA amplification made possible with a TwistAmp® Basic kit. Contains all the enzymes and reagents necessary for the amplification of DNA, the user needs only supply primers and template. Even PCR primers can work using TwistAmp® Basic. TwistAmp® Basic has been used for solid-phase, tailed primers, aptamers, electrochemistry and microarray applications. See manual for more information. Click to order oligonucleotides.
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Description
DNA amplification made possible with a TwistAmp® Basic kit. Contains all the enzymes and reagents necessary for the amplification of DNA, the user needs only supply primers and template. Even PCR primers can work using TwistAmp® Basic. TwistAmp® Basic has been used for solid-phase, tailed primers, aptamers, electrochemistry and microarray applications. See manual for more information. Click to order oligonucleotides.
Perfect for: End-point gel electrophoresis DNA detection, down-stream applications (e.g. sub-cloning)
Cluster of differentiation 5 (CD5) is expressed in high levels on the surface of T cells, while controversy surrounds the expression levels and role of CD5 in B cells. As a part of a diagnostic panel, its utility lies predominantly as a marker for T cells, with over 70% of T cell neoplasms expressing CD5. In particular, it is correlated with chronic lymphocytic leukemia/small lymphocytic lymphomas, mantle cell lymphoma, as well as a subset of diffuse large B cell lymphomas. CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. CD5 also has value as a prognostic indicator, being associated with poor prognosis in acute T cell lymphoblastic leukemia.
Opentrons Flex 8-Channel pipettes enable highly accurate pipetting, optimized for automation.
Flex pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 1 to 50 µL and 5 to 1000 μL. Smart sensors support automatic calibration, real-time positioning, and error detection. Choose the pipettes that fit your workflow — the gantry supports any two Flex 1-Channel or 8-Channel Pipettes — and swap them out yourself when your workflow changes.
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Opentrons Flex 8-Channel pipettes enable highly accurate pipetting, optimized for automation.
Flex pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 1 to 50 µL and 5 to 1000 μL. Smart sensors support automatic calibration, real-time positioning, and error detection. Choose the pipettes that fit your workflow — the gantry supports any two Flex 1-Channel or 8-Channel Pipettes — and swap them out yourself when your workflow changes.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
As this is a 2 gene kit, we recommend purchase of 2 of the accompanying RT-qPCR master mix reagent: oasig Lyophilised OneStep RT-qPCR Master Mix 150 reactions.
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Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
