• Ready to Use  Streptavidin Europium Chelate Microspheres
• Specifically Designed Running Buffer

Description

Europium Chelate Microspheres Nanoparticle Streptavidin Conjugate for Lateral Flow

Our Streptavidin Europium Chelate Microspheres conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin Europium Chelate Microspheres  can irreversibly bind biotin.

Europium dyed polystyrene beads conjugated to streptavidin

250ul of a 1mg/ml stock ready to use for Lateral Flow

50mL Lateral FLow Running Buffer designed for Streptavidin Europium Chelate Microspheres 

Inquire for Larger Volumes: sales@attogene.com

Excitation max: 365nm
Emission max: 610nm

Europium Chelate Microspheres Nanoparticle Streptavidin Conjugate for Lateral Flow

Our Streptavidin Europium Chelate Microspheres conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin Europium Chelate Microspheres can irreversibly bind biotin.

Introduction

Usages:
For cultivations of yeasts , moulds and acidophilic bacteria.

Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.

Formulation（per liter）:
Peptone 5g
Tryptone 5g
Glucose 20g
Final pH 5.7 ± 0.1        

How to use：
1.Suspend 30g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.

Quality control：
          　　

Item	The name and number of strain	Growth	Colony Color
1	Aspergillus niger ATCC16404	Good	Spore surface growth
2	Candida albicans ATCC10231	Good	Cloudy broth
3	Escherichia coli ATCC25922	Inhibition	—

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle

250g

Overview

• Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
• Versatile sample input ranging from 50 µL to 10 mL
  • Plasma/Serum Exosome Purification Mini Kit (50 µL – 1 mL Plasma/Serum)
  • Plasma/Serum Exosome Purification Midi Kit (1 mL – 4 mL Plasma/Serum)
  • Plasma/Serum Exosome Purification Maxi Kit (4 mL – 10 mL Plasma/Serum)
• Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
• No precipitation reagents, overnight incubation, protease or coagulant treatments required
• No time-consuming ultracentrifugation, filtration or special syringes required
• Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
• Purified exosomes are compatible with functional studies.
• Purified exosomes are free from any RNA-binding proteins
• Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
• Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.

The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.

NanoSight® Analysis

Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration

Exosomal RNA Analysis

To purify exosomes and isolate exosomal RNA, choose the Plasma/serum Exosome Purification and RNA isolation kits. The protocol is divided into 2 parts and an aliquot of purified exosomes can be taken for applications like NTA/TEM etc. before processing them for RNA isolation. Or you can use the Exosomal RNA Isolation Kit if you’ve already purified exosomes using a Norgen kit or another method. . Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. This RNA is ideal for gene expression analysis using RT-qPCR, microarray, or NGS and for biomarker discovery.

Details

Supporting Data

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Kit Specifications
Plasma/Serum Input (Cat. 57400)	50 μL – 1 mL
Plasma/Serum Input (Cat. 57500)	1 mL – 4 mL
Plasma/Serum Input (Cat. 57600)	4 mL – 10 mL
Size of Exosomes Purified	40 nm – 150 nm
Elution Volume	Variable depending on the plasma/serum input volume
Time to Complete 10 Purifications	15 – 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Component	Cat. 57400 (50 preps)	Cat. 57500 (25 preps)	Cat. 57600 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	8 mL	2 x 8 mL
ExoR Buffer	12 mL	12 mL	12 mL
Mini Filter Spin Columns inserted into 2 mL tubes	50	25	15
Product Insert	1	1	1