Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1 fg/μL, 0.3 fg/μL, 0.5 fg/μL, 1 fg/μL and 3 fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentrati on values of samples with 0.3 fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
Name of Product
HybriDetect Cassette
Catalog Number
MGHC 1
Short Info
The HybriDetect Cassette is the cassette version of our HybriDetect dipstick (MGHD 1). It is a simple and quick tool to develop your own rapid test and allows you and your clients a custumor friendly evaluation. Various molecules can be detected such as proteins, antibodies and genetic amplicons.
Please note, that you may have to pay country-specific taxes and duties.
Method/Platform
Lateral flow, sandwich or competetive
Range/Assay Sensivity
10 pmol/LFA – 1 fmol/LFA
Test Principle
Milenia HybriDetect Cassette is a ready-to-use, universal test unit, which is based on the lateral flow technology using gold nanoparticles. The test unit is designed to develop qualitative or semi-quantitative rapid test systems for several analytes such as proteins, antibodies, or gene amplicons. The user needs to develop an analyte-specific solution or reaction (PCR, RPA, LAMP, CRISPR/Cas, …), which contains a first detector (e.g. antibody, antigen, specific probe) labelled with FITC or FAM and a second one (e.g. antibody, primer) labelled with biotin.
The HybriDetect Cassette is the cassette version of our HybriDetect dipstick (MGHD 1). It is a simple and quick tool to develop your own rapid test and allows you and your clients a custumor friendly evaluation. Various molecules can be detected such as proteins, antibodies and genetic amplicons.
Bacillus cereus is a rod-shaped, gram-positive bacterium. Some strains of B. cereus cause foodpoisoning and other diseases such as keratitis. B. cereus grows at a wide range of temperature, from 4°C to 37°C. In fact, many foodborne illnesses caused by B. cereus are a consequence of improperly cooked or improperly stored food, including dairy products and meats. The majority of food poisoning by B. cereus could be divided into two types of symptoms – the emetic type and the diarrheal type, both of which are caused by the enterotoxin produced by the bacteria.
Bacillus cereus TaqMan PCR Kit,
100 reactions
Bacillus cereus TaqMan PCR Probe/Primer Set and Controls,
100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM36950 (100 preps) | Cat. TM36910 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Bacillus cereus Primer & Probe Mix | 280 μL | 280 μL |
| Bacillus cereus Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |