2×1×96well Microplate Box Type Rotor 

Bring your assay kits to the next level with freeze-dried PCR beads.

LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.

LyoBeads contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which  makes reaction setup very simple. Only a biological sample has to be added.

Storage: LyoBeads are shipped and stored simply at room-temperature. This provides a more cost efficient and ecological distribution compared to other master mixes.

LyoBeads can be pre-dispensed in PCR-strips, PCR-plates, cartridges etc.

Example performance data can be downloaded here.

As this is a customized product, we take your requirements very serious. You would like to know more? Please get in touch! 

Bring your assay kits to the next level with freeze-dried PCR beads.

LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.

LyoBeads contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very simple. Only a biological sample has to be added.

Overview

• Detection kits for Legionella sp.
• Available for TaqMan format for analysis

Norgen’s Legionella sp. TaqMan PCR Kit is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of Legionella sp. specific DNA is based on TaqMan PCR providing a simple, reliable and rapid result for the detection of Legionella sp. infection. Norgen’s Legionella sp. TaqMan PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Legionella sp. TaqMan PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

Legionella sp. TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Legionella sp. TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM64450 (100 rxns)	Cat. TM64410 (100 rxns)
MDx TaqMan 2X PCR Master Mix	2 x 700 mμL	–
Legionella sp. Primer & Probe Mix	280 μL	3 x 70 μL
Legionella sp. Positive Control	150 μL	3 x 50 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description 

The PM1500 ExcelBand™ All Blue Regular Range Protein Marker is a blue protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).

The PM1500 ExcelBand™ All Blue Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Two enhanced bands — 72 kDa and 25 kDa

Contents

Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control

Under suggested conditions, PM1500 ExcelBand™ All Blue Regular Range Protein Marker resolves 10 major bands in SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for long term storage 

The PM1500 ExcelBand™ All Blue Regular Range Protein Marker is a blue protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).

The PM1500 ExcelBand™ All Blue Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.