Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is the etiological agent of the sexually transmitted infection (STI) gonorrhoea, which globally causes an estimated 60 million new cases of gonococcal disease annually. It is second only to Chlamydia trachomatis as the most reported notifiable sexually transmitted disease. Infections with N. gonorrhoeae are primarily restricted to the mucus membranes of the endocervix, urethra, rectum, and pharynx. In females, gonorrhoea is a major cause of pelvic inflammatory disease and may lead to tubal infertility, ectopic pregnancy, and chronic pelvic pain, whereas in males it primarily causes urethritis. Importantly, these infections may often be asymptomatic, thereby contributing to further transmission and maintenance of the disease within populations.
Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard is prepared from pelleted bacteria grown on culture plates using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Neisseria gonorrhoeae.
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Volume Provided – 250 µL
DNA Quantity – 2 x 104 copies per µL
Storage Conditions
Upon receipt, store Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20oC or lower.
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Product Description
High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids
Product parameters:
Reagent component (WLD8201-ES,2Tubes/box) | ||
Component | Specification | Quantity |
LD-1 | 800μL=0.8mL | 1 Tube |
LD-2 | 200μL=1.2mL | 1 Tube |
Product features and advantages:
Product features | |
Simplicity | The operation is simple, and the DNA template for amplification can be obtained in just one step (conventional 5min) |
Security | Non-toxic environmental protection, room temperature transport and storage |
Universality | The lysate can be used for isothermal nucleic acid amplification |
Product application:
It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.
Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.
Sample processing type | |
Liquid sample | Samples of plants or tissues |
Serum, blood, saliva, ascites and other samples, swab leach solution | Brain, muscle, viscera, leaves, fruit and other samples, take liquid supernatant |
Norgen’s AAV Quantification Kit is designed for the detection of adeno-associated virus (AAV) inverted terminal repeat (ITR) sequences in a real-time PCR based on the use of TaqMan® technology. A purified standard based solely on the AAV2 ITR sequence purified using Norgen’s proprietary silicon carbide technology simplifies the generation of a reliable standard curve for AAV quantification. Avoidance of a plasmid based standard eliminates problems associated with efficient melting of the ITR sequence due to coupling of the ITR to the much longer plasmid sequence, as well as variability due to rearrangements/duplications/deletions of the recombination prone ITR. An easy and rapid method for viral DNA extraction simplifies the step of obtaining AAV DNA while simultaneously eliminating contaminating non-encapsidated DNA. Norgen’s AAV Quantification Kit can facilitate pre-clinical studies that require accurate vector titration as well as interlab comparisons of vector quantities. This kit is designed for research use only and not for use in diagnostic procedures.
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Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
Component | Cat. 63800 (24 rxns) |
---|---|
Enzyme Incubation Buffer A | 500 µL |
DNase I | 25 µL |
DNase Stop Solution | 100 µL |
2X PCR Mastermix | 350 µL |
AAV Primer & Probe Mix | 90 µL |
AAV Standard | 6 µL |
Nuclease-Free Water (Negative Control) | 1.25 mL |
Product Insert | 1 |
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