Product Description
RNA Amplification Kit With Improved Detection & Quantification-20°C Format Easy And Efficient
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters | Details |
---|---|
Product Name | RNA Isothermal Amplification Kit NFO |
Manufacturer | Amp-future |
Storage Temperature | -20°C |
Kit Components | Enzymes, Buffers ,Reagents |
Packaging | 48 Tests/box |
Detection Limit | 500-1000copies/µL |
Shipping | ICE |
Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Usages:
For monitoring and detection of surface of equipment and personnel hygiene which have disinfectant or antibiotics.
Advantage:
1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life.
2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability.
3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation.
4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit..
5.This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.
Storage: Store in a cool (2-25 ℃), dry place, away from bright light. Storage period of 6 months.
Specifications: 55mm*10 plates / bag
55mm*10 plates / bag
Description
The DM3160 FluoroBand™ 1KB (0.25-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA ladder DM3160 is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
250 ~ 10,000 bp
Concentration
50 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light
Room temperature for 6 months
4°C for 12 months
-20°C for 24 months
The DM3160 FluoroBand™ 1KB (0.25-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA ladder DM3160 is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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