This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
This kit can use on manual protocol or 16/32/48 channel automated extraction system. No need to pause for the addition of binding solution (not applicable to 96/192 instruments)
Contents | D631001C | D631002C | D631003C |
Purification Times | 48 | 96 | 480 |
MagPure Particles | 1.7 ml | 3.5 ml | 16 ml |
Proteinase K | 24 mg | 50 mg | 220 mg |
Protease Dissolve Buffer | 1.8 ml | 3 ml | 15 ml |
Buffer MLA | 30 ml | 70 ml | 300 ml |
Buffer DW1 | 30 ml | 70 ml | 300 ml |
Buffer EW | 60 ml | 120 ml | 2 x 300 ml |
Buffer MWX1 | 30 ml | 70 ml | 300 ml |
Elution Buffer | 10 ml | 20 ml | 60 ml |
Prefilled Kit Contents
Product | Contents and volume | D6310-TL-06C | D6310-S-48 |
Proteinase K | 50 mg | 24 mg | |
Protease Dissolve Buffer | 3 ml | 1.8 ml | |
TL-Tip | 12 pcs | 24 pcs | |
Tip bottom plate/ Tip base reagent strip | Row 1/7:600μl Buffer MLA | 6 plates | 48 strips |
Row 2/8:600μl Buffer MWX1 | |||
Row 3/9:600μl Buffer DW1 | |||
Row 4/10:30μl MagPure Particles 600μl Buffer EW | |||
Row 5/11:600μl Buffer EW | |||
Row 6/12:100μl Elution Buffer |
Storage and Stability
Proteinase K Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.
This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
The Opentrons Flex Deck Expansion Set (4 count) allows you to utilize up to 4 more positions on the Opentrons Flex worktable. The expansions slots are installed to the right of the worktable, acting as positions A4, B4, C4, and D4 and accepting plates, tube racks, and other labware that support SBS dimensions.
The deck expansion slots are necessary for all high-throughput workflows and can be paired with the Opentrons Flex Waste Chute to maximize the throughput capabilities of the Opentrons Flex.
The Opentrons Flex Deck Expansion Set (4 count) allows you to utilize up to 4 more positions on the Opentrons Flex worktable. The expansions slots are installed to the right of the worktable, acting as positions A4, B4, C4, and D4 and accepting plates, tube racks, and other labware that support SBS dimensions.
The deck expansion slots are necessary for all high-throughput workflows and can be paired with the Opentrons Flex Waste Chute to maximize the throughput capabilities of the Opentrons Flex.
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