K-MRGLUC
1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
| Content: | 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Glucose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance, Fluorescence |
| Wavelength (nm): | (Fluorescence) 530-560 (ex), ~ 590 (em) (Absorbance) 570 |
| Signal Response: | Increase |
| Linear Range: | (Fluorescence) 0.009-0.18 μg of D-glucose per assay (0.5-10 μM in-assay) (Absorbance) 0.9-9.0 μg of D-glucose per assay (5-50 μM in-assay) |
| Limit of Detection: | (Fluorescence) 0.09 ug/mL (Absorbance) 0.25 ug/mL |
| Reproducibility (%): | (Fluorescence) ~ 6% (Absorbance) ~ 7% |
| Reaction Time (min): | (Fluorescence) 30 min (Absorbance) 30 min |
| Application examples: | Food and beverage samples such as wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables. Other samples such as tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.). |
This product has been discontinued.
The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.
*The excitation and emission maxima of Megaplex Red are 570 nm and 585 nm respectively.
See more related mono/disaccharide assay kit products.
Fluorometric/UV method for the determination of D-glucose content in a variety of samples.
Advantages
The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.
This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples. Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover. The kit reduces sample complexity to allow for convenient investigation of less abundant proteins. In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins. The kit is ‘generic’ in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material – human or various other animals – making it more versatile, flexible and affordable than other kits. Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more.
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| Kit Specifications | |
| Maximum Column Capacity | 500 μg |
| Minimum Column Capacity | 200 μg |
| Minimum Elution Volume | 30 μL |
| Time to Process 10 Samples | 30 minutes |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 17300 (25 preps) |
|---|---|
| Wash Solution G | 125 mL |
| Elution Buffer C | 8 mL |
| Protein Neutralizer | 4 mL |
| Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
Features
1. TD4A centrifuge is of microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, blood center, laboratory and biochemistry.
2. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.
3. The flexible axle driven system which drive the rotor directly, smooth in operation and small vibration.
4. There are many rotors for your choice, suitable for different specifications meet customers’ different requirements of separation.
5. Micro computer control system, LED display the RCF，time and speed.
6 .Electric lid lock, compact design, super speed and imbalance protection.
TD4A Technical Parameter：
| Max. Speed | 4000rpm |
| Max. RCF | 2250×g |
| Max. Capacity | 6×50ml |
| Time Range | 0~99min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 45 |
| Temperature | Normal |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±10r/min |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 485×320×255mm |
| Net Weight/Gross Weight(Kg) | 18KG/21KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TD4A
| Order No | Rotors | Max speed r/min | Volume(ml) | Max RCF(g) |
| No31601 | Angle Rotor | 4000 | 12×10ml Vacuum tube | 2150 |
| 12×7ml Vacuum tube adapter | ||||
| 12×5ml Vacuum tube adapter | ||||
| No31605 | Angle rotor | 4000 | 30×7/5ml | 2250 |
| No31604 | Angle rotor | 4000 | 18×10ml | 2250 |
| No31607 | Angle rotor | 4000 | 24x10ml | 2200 |
| No31602 | Angle rotor | 4000 | 12×15ml/7ml/5ml | 2150 |
| No31603 | Angle rotor | 4000 | 12×20ml | 2220 |
| No31606 | Angle rotor | 4000 | 6x50ml | 2100 |