The FastRunner DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains eight discrete fragments ranging from 50 bp to 2000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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FastRunner DNA Ladder (Cat# 12800) – 100 loads
Ladder Properties:
• Eight discrete bands, ranging from 50 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference
| Fragment | Size (bp) | Mass (ng) |
| 1 | 2000 | 104 |
| 2 | 1500 | 88 |
| 3 | 1000 | 68 |
| 4 | 750 | 59 |
| 5 | 500 | 93 |
| 6 | 300 | 28 |
| 7 | 150 | 35 |
| 8 | 50 | 25 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Solid Phase Adsorption Toxin Tracking (SPATT) is a biomimetic in-situ water monitoring tool that falls under an expanding umbrella of passive samplers. It serves to warn researchers of toxin-producing harmful algal bloom (HAB) developments early on. It has been popularized through its affordability, ease of use, and its ability to capture ephemeral events in marine, brackish, and freshwater environments. Its uptake of contaminants has been shown to be more similar than other sampling methods to that of aquatic species like bivalves, mussels, and clams. It provides an average bioavailable fraction of a toxin over deployment time that can be used to determine an overall toxin risk to organisms. The sampling period typically depends on the bioactivity at a site, ranging from 24 hours to 4 weeks in most cases.
A SPATT passively absorbs and desorbs extracellular compounds over its stretch of time at a sampling site; in an organism, a toxin would go through biochemical detoxification processes. Passive samplers have a higher sensitivity for more compounds and provide improved stability and preservation of these compounds within the resin. SPATT devices capture less commonly detected cyanotoxins (e.g. cylindrospermopsin) at lower concentrations than that of a grab sample (collected at one point in time). Grab samples are limited in scope and sensitivity, and underrepresent toxins like microcystin-LR, which is picked up very reliably through SPATT technology.
Uses HP20 that is widely applicable for many toxins.
Used to capture:
Set of three Solid Phase Adsorption Toxin Tracking (SPATT) Bags
Pre activated
Ready for deployment
HP20
Strong adhesive aluminium PCR foil seal which is pierceable, peelable and suitable for high temperature applications.
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