

Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
GL20 Technical Parameter:
| Max. Speed | 21000rpm |
| Max. RCF | 30910×g |
| Max. Capacity | 4×800ml |
| Time Range | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 60 |
| Temperature | -20℃~40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 680×540×830mm |
| Net Weight(Kg) | 170KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for GL20
| Order No | Rotor | Rotor Max Speed(r/min) | Volume(ml) | RCF(×g) |
| G20-1 | Swing Rotor | 4000 | 4×800ml | 3450 |
| G20-2 | Microplate rotor | 4000 | 4×4×96 well | 2940 |
| G20-3 | Microplate rotor | 4000 | 2×4×96 well | 3210 |
| G20-4 | Microplate rotor | 4000 | 2×3×48 well | 2300 |
| G20-5 | Swing rotor | 4000 | 4×40×7ml vacuum tube | 3270 |
| G20-6 | 4000 | 4×40×5ml vacuum tube | 2890 | |
| G20-7 | 4000 | 4×28×10ml vacuum tube | 3130 | |
| G20-8 | Swing Rotor | 4000 | Fat bottle | 3830 |
| G20-9 | Fixed Rotor | 16000 | 4×8PCR | 15760 |
| G20-10 | Fixed Rotor | 15000 | 6×8PCR | 21420 |
| G20-11 | Fixed Rotor | 16000 | 8×8PCR | 17480 |
| G20-12 | Fixed Rotor | 15000 | 12×8PCR | 22930 |
| G20-13 | Fixed Rotor | 15000 | 40×0.5ml | 22920 |
| G20-14 | Fixed Rotor | 21000 | 12×1.5ml | 30910 |
| G20-15 | Fixed Rotor | 16000 | 24×1.5ml | 23440 |
| G20-16 | Fixed Rotor | 14000 | 30×1.5ml | 20800 |
| G20-17 | Fixed Rotor | 13000 | 48×1.5ml | 17930 |
| G20-18 | Fixed Rotor | 16000 | 16×5ml | 22020 |
| G20-19 | Fixed Rotor | 16000 | 6×10ml | 21500 |
| G20-20 | Fixed Rotor | 15000 | 12×10ml | 22680 |
| G20-21 | Fixed Rotor | 13000 | 16×10ml | 19490 |
| G20-22 | Fixed Rotor | 13000 | 8×15ml | 17790 |
| G20-23 | Fixed Rotor | 11000 | 12×15ml | 14330 |
| G20-24 | Fixed Rotor | 5000 | 24×15ml | 3500 |
| G20-25 | Fixed Rotor | 5000 | 30×15ml | 3830 |
| G20-26 | Fixed Rotor | 14000 | 6×30ml | 19060 |
| G20-27 | Fixed Rotor | 13000 | 6×50ml(conical) | 18840 |
| G20-28 | Fixed Rotor | 13000 | 6×50ml(round) | 18730 |
| G20-29 | Fixed Rotor | 5000 | 12×50ml | 3860 |
| G20-30 | Fixed Rotor | 4000 | 24×50ml | 2970 |
| G20-31 | Fixed Rotor | 13000 | 4×85ml | 18940 |
| G20-32 | Fixed Rotor | 12000 | 4×100ml | 14850 |
| G20-33 | Fixed Rotor | 10000 | 6×100ml | 11380 |
| G20-34 | Fixed Rotor | 4000 | 12×100ml | 2970 |
| G20-35 | Fixed Rotor | 12000 | 24 capillaries | 15800 |
| G20-36 | Swing Rotor | 15000 | 4×5ml | 19920 |
| G20-37 | Vertical rotor | 16000 | 16×5ml | 16450 |
| G20-38 | Vertical rotor | 14000 | 8×30ml | 19750 |
Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.
7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.
The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
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Click for expanded view
| Kit Specifications – Spin Column | |
| Column Binding Capacity | 13 μg |
| Maximum Column Loading Volume | 650 μL |
| Maximum Amount of Starting Material | 25 μg |
| Time to Complete 10 Purifications | 10 minutes |
| Minimum Elution Volume | 30 μL |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 34500 (50 preps) | Cat. 34400 (2 x 96-well plate) |
|---|---|---|
| Binding Buffer C | 30 mL | 3 x 30 mL |
| Wash Solution A | 12 mL | 2 x 20 mL |
| Elution Buffer B | 8 mL | 2 x 15 mL |
| Spin Columns | 50 | – |
| Collection Tubes | 50 | – |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Plate | – | 2 |
| Adhesive Tape | – | 4 |
| 96-Well Collection Plate | – | 2 |
| 96-Well Elution Plate | – | 2 |
| Product Insert | 1 | 1 |