Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective.
Our Individual Access Peel Heat Seal is a laminate seal compatible with polypropylene plates, featuring 96 individual foil seal spots or 12 strips of individual spots on a removable backing.
These seals result in individually sealed tubes/strips, and they can be removed from polypropylene plates by peeling, even with a plate which has been removed directly from -80°C storage.
Individual Access Peel Heat Seal forms a complete seal to a plate enabling very low temperature uses, including very low temperature storage, and high temperature uses, such as PCR (when used with a pressurized heated lid).
The seal demonstrates moderate solvent resistance and can be utilized for short term compound storage at room temperature.
This seal is available as sheets, for use with manual and semi-automated sealers, such as our Semi-Automated Sheet Heat Sealer (using the 59-2005 Individual Access adapter).
Peelable heat sealing foil, 96 individual seals with tabs, or 12 strips each covering 8 wells; suitable for very low-temperature storage/high temperature uses/PCR
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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| Kit Specifications | |
| Maximum Binding Capacity Per Column | Up to 50 μg RNA |
| Maximum Loading Volume Per Spin Column | 650 μL |
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications | 25 minutes |
| Maximum Amount of Starting Material | |
| Liver Tissue | 20 mg |
| Heart Tissue | 5 mg |
| Kidney Tissue | 20 mg |
| Brain Tissue | 25 mg |
| Lung Tissue | 20 mg |
| Spleen Tissue | 20 mg |
| Whole Blood | 100 µL |
| Hela Cells | 3 x 106 |
| CHO | 3 x 106 |
| Yeast | 1 x 108 cells |
| E.coli | 1 x 109 CFU/mL |
| Bacteria | 1 x 109 cells |
| Plasma/Serum | 200 µL |
| Fungi | 50 mg |
| Plant Tissue | 50 mg |
| Viruses | ≤100 µL of viral suspension |
| Average Yield | |
| HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg | |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 48300 (50 preps) | Cat. 48400 (100 preps) |
|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL |
| RNA Purification Columns | 50 | 100 |
| Genomic DNA Removal Columns | 50 | 100 |
| Collection Tubes | 100 | 200 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |