DBCO-NHCO-PEG13-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG13-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
SD-25/SD-25C Vacuum Centrifugal Concentrator
Ambient temperature type/Refrigerated type
Technical Parameter
| Temperature range | Room Temperature~+80℃ | -10℃~+80℃ |
| Temperature Accuracy | 士1℃ | |
| Max.Speed | 2500rpm | |
| Max.Capacity | 6x100ml | |
| Timer | 0~99h59min | |
| Vacuum control range | 0.1~1000mbar | |
| Main unit withstands vacuum levels | 0.01mbar | |
| Noise | ≤55dBA | ≤65dBA |
| Voltage | 0.7kw | 1.3kw |
| Power supply | 220v,50Hz | |
| Dimension(L×W×Hmm) | 360*448*304mm | 440*653*420mm |
Adapter Rotor
| Rotor Type | Rotor Volume | Rotor Type | Rotor Volume |
| Angle Rotor | 42×1.5/2ml | Angle Rotor | 8×50ml |
| Angle Rotor | 108×1.5/2ml | Angle Rotor | 6×100ml |
| Angle Rotor | 48×5ml | Angle Rotor | 16*20ml Sample vial |
| Angle Rotor | 30×10ml | Angle Rotor | 16*5ml+8*4ml+8*3ml |
| Angle Rotor | 24×15ml | Microplate Rotor | 2×96well |
Key features：
1. High-definition LCD touch screen, simultaneously displaying operational parameters: speed, temperature, vacuum, and running time.
2. Delayed start: after reaching a fixed speed, Start the vacuum pump to effectively prevent sample mixing.
3. Fully automatic vacuum control system, vacuum setting range0.1~1000mbar, Control accuracy0.1mbar.
4. 1.5/2ml Rotor stacking for simultaneous use; rotor replacement without tools.
5. Programmable with temperature and pressure settings; capable of storing 36 programs
6. Compatible with various rotor heads; transparent glass window for easy observation.
7. Magnetic drive: maintenance-free, non-contact drive, fully sealed.
8. Safety in use: inductive electric self-suction door lock ensures the centrifugal concentrator can only start when the door cover is fully closed and locked.
9. Can be used in conjunction with freeze-drying systems
Model:
SD-25/SD-25C
Brand:
Yingtai
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Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.
Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.
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Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.
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