Description

Specifications

Clone	IHC002
Source	Mouse Monoclonal
Positive Control	Breast Carcinoma
Dilution Range	1:200

The Her2/Neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between Her2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.

Introduction

This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 1-6ml plasma, serum, body fluids
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	1-6ml
Elution volume	≥50μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labour
• High purity – OD 260 / 280 : 1.7-1.9, OD 260 / 230 :1.5-2.0

Kit Contents

Contents	IVD5435
Purification Times	50
MagPure Particles F	14 ml
Carrier RNA	310 μg
Proteinase K	240 mg
Protease Dissolve Buffer	15 ml
Buffer SDS	15 ml
Buffer MLK	500 ml
Buffer MAW1	250 ml
Buffer MW2*	50 ml
Elution Buffer	60 ml

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance.Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.