endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.

endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.

Introduction

This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 50mg simple plant using 96 well bind plate
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic plant samples
Sample amount	Fresh / frozen plant samples: ≤50mgDried plant / seed samples: ≤15mg
Elution volume	≥75μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, blot hybridization, etc.
• High throughput – 96 samples can be processed simultaneously by 96 well plate
• High permeability – using composite membrane technology, no hole plugging

Kit Contents

Contents	D316702	D316703
Purification Times	4 x 96 Preps	20 x 96 Preps
RNase Solution	5 ml	22 ml
Elution Buffer	120 ml	500 ml
Buffer SPL	200 ml	2 x 500 ml
Buffer PS	100 ml	400 ml
Buffer GW1	220 ml	5 x 220 ml
Buffer GW2	100 ml	3 x 100 ml
1.6ml Collection Plate	4	20
HiPure gDNA Plate	4	20
2.2ml Collection Plate(DW Plate)	8	40
0.8ml Collection Plate(DW Plate)	4	20
Sealing Film	20	100

Storage and Stability

RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg

This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.

The Permagen 96-Well Side Pull Bar Magnet PCR Separation Plate was designed for use in manual or automation applications where pulling beads to one side of the wells is essential. Also available in a kit form which includes our MTP96 clear top plate for 0.2 mL PCR tubes and strips.  

Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added two angled, partial frames around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets when using an automated liquid handling robot.

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

MSP750 (Magnet Plate only)

MSP750KT (Includes top plate kit)

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Beads are pulled to tube walls for easy removal
• Made in USA 
• For Research use only

Compatibility

• Full – Skirt PCR plates
• Semi- Skirted PCR plates 
• Non- Skirted PCR plates
• 0.2 mL PCR Strips and tubes  (With kit)
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Minimum – 30 µL 

Maximum – 0.2 mL

The Permagen 96-Well Side Pull Bar Magnet PCR Separation Plate was designed for use in manual or automation applications where pulling beads to one side of the wells is essential. Also available in a kit form which includes our MTP96 clear top plate for 0.2 mL PCR tubes and strips.