Permagen’s 32-tube PCR Separation rack features all of the same aspects of our MSR812 rack above for easy protocol transition for labs wishing to scale up to four PCR strips at a time, or up to 32 individual PCR tubes.
Detail
Permagen’s 32-tube PCR Separation rack features all of the same aspects of our MSR812 rack above for easy protocol transition for labs wishing to scale up to four PCR strips at a time, or up to 32 individual PCR tubes.
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
Propargyl-PEG4-5-nitrophenyl carbonate is heterobifunctional reagent with a propargyl group and nitrophenyl group. The propargyl group enales the formation of triazole linkage with azide compounds or biomolecules via copper catalyzed Click Chemistry. Nitrophenyl group is reactive towards the amino group of lysine by means of stable urethane linkages. The PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG4-5-nitrophenyl carbonate is heterobifunctional reagent with a propargyl group and nitrophenyl group. The propargyl group enales the formation of triazole linkage with azide compounds or biomolecules via copper catalyzed Click Chemistry. Nitrophenyl group is reactive towards the amino group of lysine by means of stable urethane linkages. The PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Nuclease-Free Water is free of all DNases, RNases and nucleic acids and is suitable for all molecular biology applications requiring nuclease-free water including PCR, RT-PCR and real-time PCR. Our water is prepared without the use of DEPC, and is ready to use without further treatment.
The Kit integrates phenol/guanidine-based lysis and silica membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg animal, plant, fungal samples,bacteria, cells and other samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA (include miRNA) from 100mg lipid tissue, tissue, cell, plant, body fluids using columns and MagZol reagent
Applications
RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Tissue samples (10-100mg) are homogenized in MagZol Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.
Advantages
Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – several samples can be extracted in 30 minutes
High applicability – samples including animals, plants, bacteria, cells, etc.
High output – enable to process large samples and the yield can be up to 200μg
Kit Contents
Contents
R413002
D413003
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
50
250
MagZol Reagent
60 ml
270 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
The Kit integrates phenol/guanidine-based lysis and silica membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg animal, plant, fungal samples,bacteria, cells and other samples.
