384-Well Post magnetic low elution plate with integrated cushion base
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The Permagen 384-well post magnet plate was designed for use in manual or automation applications for Low Elution high throughput PCR
Detail
The Permagen 384-well post magnet plate was designed for use in manual or automation applications for Low Elution high throughput PCR
Magnetic beads are pulled midway up onto well wall to help eliminate accidental bead pellet disruption and to achieve lower volumes
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, compatible with any magnetic beads, and integrated cushion base to help aid in robot/ consumable inconsistencies
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from tissue, cell, whole blood
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type
Tissues, cells, lymphocytes and other clinical sample
Sample amount
Cells grown in suspension: ≤3 x 106Animal tissue: ≤10mgPlant tissue: ≤30mgWhole blood: 0.5~1.0 ml fresh blood or bone marrow and fresh blood mixture
Kit Contents
Contents
IVD3020B-96
IVD3020B
Purification Times
96 Preps
200 Preps
MagPure Particles N
2.5 ml
5 ml
DNase I
2 x 600 µl
4 x 600 µl
DNase Buffer C
60 ml
120 ml
Buffer RLC
60 ml
120 ml
Buffer MCB*
60 ml
150 ml
Buffer GW1*
44 ml
110 ml
Buffer MW2*
50 ml
100 ml
RNase Free Water
20 ml
60 ml
Storage and Stability
DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.
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This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
Discover our Yeast Extract Peptone Dextrose (YPD) Agar, designed for the cultivation and growth of yeasts and fungi, including Saccharomyces cerevisiae. This nutrient-rich medium supports robust microbial proliferation, making it ideal for molecular biology, fermentation, and genetic research.
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Discover our Yeast Extract Peptone Dextrose (YPD) Agar, designed for the cultivation and growth of yeasts and fungi, including Saccharomyces cerevisiae. This nutrient-rich medium supports robust mi……
Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:
Cat # 28113 – 100 reactions (sufficient for 100 reactions x 20 µL reaction volume) Cat # 28114 – 200 reactions (sufficient for 200 reactions x 20 µL reaction volume) Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)
2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) – Sufficient reagent for 200 x 20 µL reactions
Storage Conditions and Product Stability 2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.
