Specially designed custom low volume, low profile/slim 384-well plates designed for use with our GeneArrayer. These plates are made from durable polypropylene delivering an excellent low fluorescence background interference and high performance when paired with our high-throughput genotyping instruments.
The unique, slimline plate design permits lower reaction volumes than standard 384-well plates (typically 1.6 – 2.0 µL) allowing for reaction miniaturization, with all the benefits this brings saving time, consumables and costs. This plate is designed specifically to be used with 3CR Bio’s GeneArrayer automated liquid handling instrument.
Plate Dimensions: Length 127.85 mm, Width 85.85 mm, Thickness 5.05 mm, Well Spacing 4.50 mm, Individual Well Capacity 3 µL.
Other Products
NEST 50 mL Centrifuge Tube (500 count)
Product Info
Document
Product Info
NEST 50 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Printed graduations in marking area. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Document
NEST 50 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Printed graduations in marking area. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Hepatitis C Virus (HCV) TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for HCV
Available in TaqMan format for analysis
Hepatitis C virus infection is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC) worldwide. Neither a vaccine nor an effective treatment is available for HCV. Pegylated interferon-α (PEG-IFN- α), combined with ribavirin, is the current standard therapy. Its efficiency ranges between 20-80%, depending on the HCV genotype. Unfortunately, many HCV infected patients do not respond to or do not tolerate the IFN-based therapy. Therefore, the number of patients progressing to HCC, as a result of HCV infection, is expected to increase over the next 20-30 years. The natural history of the disease reveals the elusive nature of the virus in a number of features. First, the infection is often detected incidentally at the time of blood donation as the acute infection is clinically asymptomatic in most patients. Second, HCV successfully escapes multi-specific host immune responses in the majority of patients which establishes persistent infection. Third, a significant number of persistently infected individuals remain unaware of the infection for decades, until liver fibrosis, cirrhosis and/or hepatocellular carcinoma develop.
HCV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HCV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 50mg simple plant using 96 well bind plate
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, blot hybridization, etc.
High throughput – 96 samples can be processed simultaneously by 96 well plate
High permeability – using composite membrane technology, no hole plugging
Kit Contents
Contents
D316702
D316703
Purification Times
4 x 96 Preps
20 x 96 Preps
RNase Solution
5 ml
22 ml
Elution Buffer
120 ml
500 ml
Buffer SPL
200 ml
2 x 500 ml
Buffer PS
100 ml
400 ml
Buffer GW1
220 ml
5 x 220 ml
Buffer GW2
100 ml
3 x 100 ml
1.6ml Collection Plate
4
20
HiPure gDNA Plate
4
20
2.2ml Collection Plate(DW Plate)
8
40
0.8ml Collection Plate(DW Plate)
4
20
Sealing Film
20
100
Storage and Stability
RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
