Specially designed custom low volume, low profile/slim 384-well plates designed for use with our GeneArrayer. These plates are made from durable polypropylene delivering an excellent low fluorescence background interference and high performance when paired with our high-throughput genotyping instruments.
The unique, slimline plate design permits lower reaction volumes than standard 384-well plates (typically 1.6 – 2.0 µL) allowing for reaction miniaturization, with all the benefits this brings saving time, consumables and costs. This plate is designed specifically to be used with 3CR Bio’s GeneArrayer automated liquid handling instrument.
Plate Dimensions: Length 127.85 mm, Width 85.85 mm, Thickness 5.05 mm, Well Spacing 4.50 mm, Individual Well Capacity 3 µL.
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R5211 HiPure DNA/RNA/Protein Kit
Product Info
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Product Info
Introduction
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample)
Applications
SDS-PAGE electrophoresis and western blot, etc
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples
Sample amount
Cultured cells: < 10^7Animal tissue: ≤ 20 mgPlant samples: ≤ 150 mgYeast cells: 2 x 10^6 – 5 x 10^7
Principle
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
Advantages
High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
Fast – column method can complete the extraction of several samples in 30 minutes
Safe – no phenol chloroform extraction required
Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
Contents
R521102
R521103
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RLC
50 ml
200 ml
Buffer GW1*
22 ml
66 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
50 ml
3 x 50 ml
RNase Free Water
10 ml
30 ml
Elution Buffer
10 ml
30 ml
Buffer ALO(5%SDS)
10 ml
30 ml
Storage and Stability
HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Document
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases, the gold tagged RNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase is detected). When RNases are present, however, the RNA substrate is cleaved, and the 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases in both liquid and on solid surfaces and a perfect tool for monitoring mRNA vaccine manufacturing.
Document
RNase activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase contamination of materials and supplies.