Description
The DM4100 ExcelBand™ XL 25 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM4100 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
250 ~ 25,000 bp
Concentration
51.6 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
The DM4100 ExcelBand™ XL 25 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM4100 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
Norgen’s Fecal DNA Collection & Preservation Mini Tubes are designed for the rapid preservation of DNA from fresh fecal specimens. These tubes contain Norgen’s Fecal DNA Preservative in a liquid format. Moreover, each collection tube is preloaded with beads for effective sample homogenization in any commercially available bead beater equipment (e.g. MP Biomedicals’ FastPrep®-24). The user simply collects fecal samples into the tubes and mixes by shaking or vortexing until the feces is mixed well with preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow the DNA in the samples to be preserved at room temperature for over 2 years.
This kit is highly suited to collection and preservation of fecal material from mouse, hamster, chinchilla, rabbit and more for microbiome studies and other applications.
DNA Isolation from Preservative
Fecal samples collected and preserved in Norgen’s Fecal DNA Collection and Preservation Mini Tubes are compatible with most fecal DNA isolation methods. Samples stored in the tubes have been used successfully with all of Norgen Biotek’s stool-based DNA isolation kits (please refer to Related Products table) and reagents.
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| Kit Specifications | |
| Fecal Input | Maximum 30 mg (approximately 3-5 mouse droppings) |
| Stability of Fecal DNA at Room Temperature | 2 years |
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
| Kit Components | Cat. 27650 |
|---|---|
| Fecal DNA Collection & Preservation Mini Tubes (containing liquid preservative and beads) | 12 |
| Product Insert | 1 |
Opentrons Flex 1-Channel pipettes enable highly accurate pipetting, optimized for automation.
Flex pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 1 to 50 µL and 5 to 1000 μL. Smart sensors support automatic calibration, real-time positioning, and error detection. Choose the pipettes that fit your workflow — the gantry supports any two Flex 1-Channel or 8-Channel Pipettes — and swap them out yourself when your workflow changes.
Opentrons Flex 1-Channel pipettes enable highly accurate pipetting, optimized for automation.
Flex pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 1 to 50 µL and 5 to 1000 μL. Smart sensors support automatic calibration, real-time positioning, and error detection. Choose the pipettes that fit your workflow — the gantry supports any two Flex 1-Channel or 8-Channel Pipettes — and swap them out yourself when your workflow changes.