Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of DNA and RNA products.
Formats (strep gold conjugate pad):
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM/FITC-labelled primer during amplification. Test line: anti-biotin, Control Line: GAM
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC/FITC and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 GAM.
Inquire about custom configurations: [email protected]
Kit Components
Features & Benefits
Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of DNA and RNA products.
Formats (strep gold conjugate pad):
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification. Test line: anti-biotin, Control Line: GAM
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.
Inquire about custom configurations: [email protected]
Kit Components
Features & Benefits
100 Lateral Flow Dipsticks (4.5mm)
20 mL Assay running buffer
100 wells with support plate
Controls
DBCO-NHCO-PEG4-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG4-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
| Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
|---|---|---|---|---|---|
| 55000 | Plasma/Serum RNA Purification Mini Kit | 10 – 25 µL | 50 µL – 1 mL | 250 µL – 1 mL | 5 – 10 mL |
| 35300 | Total RNA Purification Micro Kit | 20 – 50 µL | 1 – 4 mL | 2 – 10 mL | 10 – 20 mL |
| 17200 | Total RNA Purification Kit | 50 – 100 µL | 4 – 10 mL | 11 – 30 mL | 20 – 35 mL |
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| Kit Specifications | |
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: | |
| Animal Cells | 1 x 106 cells |
| Animal Tissues | 10 mg |
| Blood | 100 μL |
| Plasma/Serum | 150 μL |
| Bacteria | 1 x 109 cells |
| Yeast | 1 x 108 cells |
| Fungi | 40 mg |
| Plant Tissues | 40 mg |
| Time to Complete 96 Purifications | 30 minutes |
| Average Yields* | |
| HeLa Cells (1 x 106 cells) | 15 μg |
| E. coli (1 x 109 cells) | 50 μg |
* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 17200 (50 preps) | Cat. 37500 (100 preps) | Cat. 17250 (250 preps) | Cat. 17270 (500 preps) | Cat. 24300 (192 preps) | Cat. 24370 (576 preps) | Cat. 24350 (192 preps) | Cat. 24380 (576 preps) |
|---|---|---|---|---|---|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL | 175 mL | 350 mL | 2 x 40 mL | 350 mL | 2 x 40 mL | 350 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 148 mL | 1 x 148 mL 1 x 74 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL |
| Elution Solution A | 6 mL | 2 x 6 mL | 30 mL | 60 mL | 2 x 20 mL | 60 mL | 2 x 20 mL | 60 mL |
| Mini Spin Columns | 50 | 100 | 250 | 500 | – | – | – | – |
| 96-Well Isolation Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Isolation Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Adhesive Tape | – | – | – | – | 4 | 12 | 4 | 12 |
| Collection Tubes | 50 | 100 | 250 | 500 | – | – | ||
| 96-Well Collection Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Collection Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 | 500 | – | – | ||
| 96-Well Elution Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Elution Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |