Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

Introduction

The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column
Applications	RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
Sample amount	Cells: ≤1 x 107 Animal tissue: 1-20 mgPlant leaves: 50-150 mg Yeast cells: 5 x 106
Yield	2-100μg
Elution volume	≥50μl
Time per run	≤15 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.

Advantages

• Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – several samples can be extracted in 20 minutes by column method
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG

Kit Contents

Contents	R401102	R401103
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
RTL Lysis Buffer	50 ml	200 ml
RNA Binding Buffer	15 ml	75 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

TGL18 Tabletop 21000rpm High Speed large capacity Swing out rotor 4x800ml universal Refrigerated Centrifuge Adapters for 5/10/15/50ml tubes. And holding Angle Rotors like 24×1.5/2ml, 6x50ml, 12x15ml…in high speed separation smooth.

TGL18 Universal Refrigerated Centrifuge Application:

Holding lots of Swing Out Rotors & Angle Rotors for options. Mainly used in animal and plant molecular biology, cell biology, food safety, life science, agriculture and forest science, animal husbandry science, biological products, pharmaceutical products and other fields of ideal equipment for the separation and precipitation of samples preparation.

TGL18 Universal Refrigerated Centrifuge Features:

1. With silent-block and shock absorbers that guarantees smooth and quiet operations & fast cooling.

2. The centrifuge body is made of high-quality steel, safe and reliable.

3.  Microprocessor digital control of all functions:speed, time,

temperature, acceleration/deceleration, RCF, 10 program memory, operation fault display.

4. Electronic safety lock alarm inside, just press down the top lid until it will lock in sealed and open up after separation automatically.

5. Easy open the centrifuge lid via pneumatic springs; the operation area of the centrifuge is at the front end.

6. RPM/RCF adjustable along with the speed setting calculating automatically.

7. 10 selective acceleration and deceleration rates for options

8. The self-diagnostic system provides a protection for imbalance over speed/voltage and electronic lock fault.

TGL18 Universal Refrigerated Centrifuge Technical Parameters:

Max Speed	21,000r/min	Max RCF	30,910xg
Max Volume	4x800ml	Power supply	AC220V,50HZ,7A
Timer	1~9h/59min	Noise	≤58dBA
Dimension	700×715×420mm	Net Weight	125KG
Speed Accuracy	±20r/min	Package(W*D*H)	Plywood box: 880x800x580mm

TGL18 Universal Refrigerated Centrifuge Matched Rotors:

Order No.	Rotor type	Max.Speed (rpm)	Max.Volume (ml)	Max.RCF (xg)
No30671	Swing out rotor	4,000	4x800ml	3,450
			Adapter for 4x23x5ml	3,450
			Adapter for4x19x10nl	3,450
			Adapter for4x14x15ml	3,450
			Adapter for4x5x50ml	3,450
No30801	Angle rotor	21,000	12×1.5/2ml	30,910
No30802	Angle rotor	15,000	40×0.5ml	22,920
No30803	Angle rotor	17,000	24×1.5/2ml	26,460
No30804	Angle rotor	13,500	30×1.5/2ml	19,340
No30844	Angle rotor	13,000	48×1.5/2ml	17,930
No30805	Angle rotor	16,000	16x5ml	22,020
No30806	Angle rotor	16,000	12x7ml	21,380
No30807	Angle rotor	10,000	12x15ml	11,840
No30808	Angle rotor	15,000	12x10ml	22,680
No30809	Angle rotor	15,000	8x20ml	22,680
No30811	Angle rotor	13,000	6x50ml	18,840
No30814	Angle rotor	12,000	6x100ml	16,390

The Corners of our Company____________________________________

Metal housing with 3-tiers protection. It can hold both swing out rotors and angle rotors in low speed and high speed spining to switch smoothly. And its Quiet noise, Less vibration & Fast cooling guarantee a perfect separation.