40×0.5ml Angle Rotor

Overview

• Fast, reproducible and easy processing of samples
• High yield and high quality genomic DNA with no RNA or protein contamination
• DNA ready for any application including PCR, qPCR, genotyping and more

Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.

Cells and Tissue DNA Isolation Kit (Spin Column)

Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Micro Kit (Micro)

Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.

Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.

Details

Supporting Data

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Kit Specifications
Maximum Cells and Tissue Input	20 mg of animal tissue 3 x 106 cells
Average Yield*	8-10 µg (20 mg of animal tissue) 8-12 µg (3 x 106 cells)
Average Purity (OD260/280)	1.8 – 1.9
Time to Complete 10 Purifications	40 minutes hands-on time (Cat. 59100) 50 minutes hands on time (Cat. 62500)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Component	Cat. 53100 (50 preps)	Cat. 57300 (50 preps)	Cat. 59100 (50 preps)	Cat. 62500 (192 preps)
Lysis Buffer B	20 mL	20 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	18 mL	18 mL	55 mL
Wash Solution A	18 mL	18 mL	–	–
Elution Buffer B	30 mL	8 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K	1.2 mL	1.2 mL	1.2 mL	–
Proteinase K in Sotrage Buffer	–	–	–	4 mL
Magnetic Bead Suspension	–	–	2 x 1.1 mL	8.5 mL
96-Well Plate	–	–	–	2
Spin Columns	50	–	–	–
Micro Spin Columns	–	50	–	–
Collection Tubes	50	50	–	–
Elution Tubes (1.7 mL)	50	50	50	–
96-Well Elution Plate	–	–	–	2
Adhesive Tape	–	–	–	2
Product Insert	1	1	1	1

Features

1.        Brushless frequency motor, in great torque, free maintenance, no power pollution, quick in speed up and down.

2.        7” LCD Touch screen display which indicates the program, rotor No, speed, time, RCF and temperature.

3.        CFC-free refrigerant, pre-cooling function ,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.

4.        Microprocessor control , 100 programs in store, 10 kinds of acceleration and deceleration for your choice.

5.         Over speed and imbalance protection.

6.        Speed, RCF, time, temperature can be edited during running.

7.        High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect sleeve, 3 tiers protection, safe and reliable.

8.        Automatic door open & close, as well as anti-pinch function (Optional).

CDL7M Technical Parameter：

Max. Speed	7000rpm
Max. RCF	11650×g
Max. Capacity	6×2400ml (12*500ml blood   bag）
Time Range	1~99h59min59s
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Temperature	-20 ~ 40℃
Acc/Dec	10 Kinds
Speed   Accuracy	±20r/min
Temperature   Accuracy	±1℃
Voltage(V/Hz)	AC 220V/380V    50HZ/60HZ
Size (W x D x Hmm)	940×890×1000mm
Net Weight(Kg)	570KG
Certificates	CE,ISO & Calibration report are available

Special rotor for blood bag separation

Order   No.	Rotor   No.	Max   Speed (rpm)	Max Volume(ml)	Max.   RCF（×g）
05202	Swing Rotor	4000	6×2x1000ml	5210

CDL7M touch screen with special programs for blood bag separation, can get good performance for platelet, red cell, all parts separation of blood. 6x2x1000ml bucket suitable for blood bag separation. Also with automatical lid lock open and close function, also wind shield can open along with door open.
Model:
CDL7M

Brand:
Yingtai

Code:
CDL7M blood bank centrifug