Norgen’s Trichomonas vaginalis End-Point PCR Kit is designed for the detection of Trichomonas vaginalis specific DNA based on the use of end-point PCR technology. This kit is designed for research use only and not for use in diagnostic procedures. The kit includes Master Mix and primers for the specific amplification of a 335 nucleotide region of the Trichomonas vaginalis genome, as well as a positive control and a negative control to confirm the integrity of the kit reagents. In addition, the kit contains loading dye and a DNA ladder to facilitate analysis of the results.
The detection of Trichomonas vaginalis specific DNA is based on end-point PCR technology, utilizing polymerase chain reaction (PCR) for the amplification of specific Trichomonas vaginalis DNA sequences. For analysis of the PCR data, the PCR reaction is loaded on an agarose DNA gel along with the provided DNA ladder for qualitative analysis.
Trichomonas vaginalis TaqMan PCR Kit, 100 reactions
Trichomonas vaginalis TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM52050 (100 preps) | Cat. TM52010 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Trichomonas vaginalis Primer & Probe Mix | 280 μL | 280 μL |
| Trichomonas vaginalis Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
DBCO-PEG12-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG12-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples. It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk. Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.
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| Kit Specifications | |
| Maximum Milk Input | 1 mL |
| Time to Complete 10 Purifications | 1 hour |
| DNA Yield* | 500 ng to 8 μg |
| Bacteria Species Processed | Gram positive and Gram negative |
| Minimum Detection Limit | 10 bacteria in 1 mL of milk |
*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.
| Component | Cat. 21550 (50 preps) |
|---|---|
| Resuspension Solution A | 6 mL |
| Buffer SK | 60 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 12 mg |
| Lysozyme (powder) | 120 mg |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
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