Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Usages:
For enriching and cultivating Clostridium.
Principle:
Meat extract powder, peptone provide nitrogen; yeast extract powder, cysteine hydrochloride, sodium acetate offers a variety of vitamins and growth factors; glucose carbon source; low concentrations of soluble starch byproduct of metabolic detoxification; sodium chloride maintain osmotic equilibrium; agar coagulant.
Formulation(per liter):
Beef extract 10g
Peptone 10g
Yeast extract 3g
Glucose Monohydrate 5g
Sodium chloride 5g
Soluble starch 1g
Cysteine Hydrochloride 0.5g
Sodium Acetate 3g
Agar 0.5g
Final pH 6.8±0.2
How to use:
1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g
Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane TFA Salt is a crosslinker consisting of an amino group with three propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane TFA Salt is a crosslinker consisting of an amino group with three propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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