029120 Lugol Iodine
Usage:For starch hydrolysis test.
Specification:10ml.
10ml
Propargyl-PEG4-methylamine is a PEG linker that can be react with azide compounds in copper catalyzed azide-alkyne Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The PEG spacer increases hydrophilicity of the linker in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-methylamine is a PEG linker that can be react with azide compounds in copper catalyzed azide-alkyne Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The PEG spacer increases hydrophilicity of the linker in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate |
| Applications | Enzyme digestion, sequencing, PCR, labeling, etc. |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Conventional plasmid, plasmid less than 30KB |
| Sample amount | 1-1.5ml（x96） |
| Yield | 1-15µg/1ml |
| Elution volume | ≥70μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 70µg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
| Contents | P100601 | P100602 | P100603 |
| Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
| RNase A | 5 mg | 20 mg | 100 mg |
| Buffer P1 | 30 ml | 120 ml | 600 ml |
| Buffer P2 | 30 ml | 120 ml | 600 ml |
| Buffer P3 | 40 ml | 180 ml | 800 ml |
| Buffer PW1 | 100 ml | 500 ml | 2 x 1000 ml |
| Buffer PW2 | 50 ml | 2 x 100 ml | 4 x 200 ml |
| Elution Buffer | 150 ml | 60 ml | 300 ml |
| Lysate Clear Plate | 1 | 4 | 20 |
| HiPure DNA Plate | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.5ml Elute Plate | 1 | 4 | 20 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.