The BK virus is a member of the polyomavirus family. It has been suggested that this virus may be transmitted through respiratory fluids or urine, since infected individuals periodically excrete virus in the urine. BK viral infections are typically asymptomatic in healthy individuals, however very mild symptoms may appear including mild respiratory infections and fever. Infections with BK virus in immunocompromised or immunosupressed patients are much more severe and may involve renal dysfunction. In fact, in kidney transplant patients the immunosupressive drugs required for the transplant may allow the virus to replicate within the graft, resulting in a disease called BK virus nephropathy (BKVN). The JC virus is a type of human polyomavirus and is very common in the general population, infecting 70 to 90% of humans. Most people acquire JCV in childhood or adolescence. Typically the infection is subclinical and no of consequence in individuals with healthy immune systems. The initial site of infection may be the tonsils or the gastrointestinal tract, and the virus then remains latent in the gastrointestinal tract. JCV can also infect the tubular epithelial cells in the kidneys, where it continues to reproduce, shedding virus particles in the urine. Also, JCV can cross the blood-brain barrier into the central nervous system. JCV is known to cause the usually fatal progressive multifocal leukoencephalopathy (PML) by destroying oligodendrocytes in the brain in immunodeficient or immunosuppressed individuals. The JC and BK viruses are very similar, with their genomes sharing 75% homology. It is however important to differentiate between the viruses due to the differences in pathology and especially the invariably fatal outcome of PML which is only caused by the JC virus.
BKV/JCV TaqMan PCR Kit, 100 reactions
BKV/JCV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM39350 (100 preps) | Cat. TM39310 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| BKV/JCV Primer & Probe Mix | 280 μL | 280 μL |
| BKV/JCV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
GL20 Technical Parameter：
| Max. Speed | 21000rpm |
| Max. RCF | 30910×g |
| Max. Capacity | 4×800ml |
| Time Range | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 60 |
| Temperature | -20℃~40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 680×540×830mm |
| Net Weight(Kg) | 170KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for GL20
| Order No | Rotor | Rotor Max Speed(r/min) | Volume(ml) | RCF(×g) |
| G20-1 | Swing Rotor | 4000 | 4×800ml | 3450 |
| G20-2 | Microplate rotor | 4000 | 4×4×96 well | 2940 |
| G20-3 | Microplate rotor | 4000 | 2×4×96 well | 3210 |
| G20-4 | Microplate rotor | 4000 | 2×3×48 well | 2300 |
| G20-5 | Swing rotor | 4000 | 4×40×7ml vacuum tube | 3270 |
| G20-6 | 4000 | 4×40×5ml vacuum tube | 2890 | |
| G20-7 | 4000 | 4×28×10ml vacuum tube | 3130 | |
| G20-8 | Swing Rotor | 4000 | Fat bottle | 3830 |
| G20-9 | Fixed Rotor | 16000 | 4×8PCR | 15760 |
| G20-10 | Fixed Rotor | 15000 | 6×8PCR | 21420 |
| G20-11 | Fixed Rotor | 16000 | 8×8PCR | 17480 |
| G20-12 | Fixed Rotor | 15000 | 12×8PCR | 22930 |
| G20-13 | Fixed Rotor | 15000 | 40×0.5ml | 22920 |
| G20-14 | Fixed Rotor | 21000 | 12×1.5ml | 30910 |
| G20-15 | Fixed Rotor | 16000 | 24×1.5ml | 23440 |
| G20-16 | Fixed Rotor | 14000 | 30×1.5ml | 20800 |
| G20-17 | Fixed Rotor | 13000 | 48×1.5ml | 17930 |
| G20-18 | Fixed Rotor | 16000 | 16×5ml | 22020 |
| G20-19 | Fixed Rotor | 16000 | 6×10ml | 21500 |
| G20-20 | Fixed Rotor | 15000 | 12×10ml | 22680 |
| G20-21 | Fixed Rotor | 13000 | 16×10ml | 19490 |
| G20-22 | Fixed Rotor | 13000 | 8×15ml | 17790 |
| G20-23 | Fixed Rotor | 11000 | 12×15ml | 14330 |
| G20-24 | Fixed Rotor | 5000 | 24×15ml | 3500 |
| G20-25 | Fixed Rotor | 5000 | 30×15ml | 3830 |
| G20-26 | Fixed Rotor | 14000 | 6×30ml | 19060 |
| G20-27 | Fixed Rotor | 13000 | 6×50ml(conical) | 18840 |
| G20-28 | Fixed Rotor | 13000 | 6×50ml(round) | 18730 |
| G20-29 | Fixed Rotor | 5000 | 12×50ml | 3860 |
| G20-30 | Fixed Rotor | 4000 | 24×50ml | 2970 |
| G20-31 | Fixed Rotor | 13000 | 4×85ml | 18940 |
| G20-32 | Fixed Rotor | 12000 | 4×100ml | 14850 |
| G20-33 | Fixed Rotor | 10000 | 6×100ml | 11380 |
| G20-34 | Fixed Rotor | 4000 | 12×100ml | 2970 |
| G20-35 | Fixed Rotor | 12000 | 24 capillaries | 15800 |
| G20-36 | Swing Rotor | 15000 | 4×5ml | 19920 |
| G20-37 | Vertical rotor | 16000 | 16×5ml | 16450 |
| G20-38 | Vertical rotor | 14000 | 8×30ml | 19750 |
Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.
7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the udder is mainly caused by infection of various bacteria. One of such mastitis bacteria, Streptococcus agalactiae, is highly infectious and causes mainly subclinical infections, which are not identified by the herdsman. As a result, S. agalactiae can spread widely within a herd, causing immediate loss due to reduced milk. S. agalactiae is a gram-positive bacteria belonging to the Group B streptococci. Traditional cultural identification of S. agalactiae is based on S. agalactiae being beta-hemolytic as well as presence of group B Lancefield antigen and by its ability to hydrolyze sodium hippurate.
Streptococcus agalactiae TaqMan PCR Kit, 100 reactions
Streptococcus agalactiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM30650 (100 preps) | Cat. TM30610 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| S. agalactiae Primer & Probe Mix | 280 μL | 280 μL |
| S. agalactiae Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |