Propargyl-PEG2-t-butyl ester is a PEG linker that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper will be required for catalyzation. Under acidic conditions, the t-butyl group can be removed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG2-t-butyl ester is a PEG linker that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper will be required for catalyzation. Under acidic conditions, the t-butyl group can be removed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Figure 1 / 7
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Kit Specifications – Spin Column | |
Column Binding Capacity | 50 μg |
Maximum Column Loading Volume | 650 μL |
Maximum Amount of Starting Material:Plant TissuesFungi (wet weight) | 100 mg 100 mg |
Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) | 18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
Plants | Plants (Cont’d) | Fungi |
Tomato | Turnip | Aspergillus niger |
Grape | Chinese Cabbage | Mucor racemosus |
Peach | Radish | Cladosporium cladosporioides |
Plum | Komatsuna | Fusarium oxysporum |
Pine Needles | Apricot | Penicillium sp. |
Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
Strawberry | Hydrangea | Pichia sp. |
Legumes | Fig | Rhizopus oryzae |
Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
Sorghum grass | Cherry | Fusarium sp. |
Tobacco | Saintpaulia | |
Arabidopsis | Lotus | |
Lichen | Carrot | |
Corn seed | Hansen | |
Sunflower seed | Pistachio | |
Olive seed | ||
Soybean seed |
Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
---|---|---|---|---|---|
Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL | 2 x 60 mL | 60 mL | 2 x 60 mL |
Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL | 25 mL | 7 mL | 25 mL |
Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL | 55 mL | 18 mL | 55 mL |
Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
Magnetic Bead Suspension | – | – | – | 4 x 1.1 mL | 2 x 8.5 mL |
Filter Columns | 50 | 250 | – | – | – |
Spin Columns | 50 | 250 | – | – | – |
Collection Tubes | 100 | 500 | – | – | – |
96-Well Plate | – | – | 2 | – | 2 |
96-Well Collection Plate | – | – | 2 | – | – |
Adhesive Tape | – | – | 4 | – | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | – | 50 | – |
96-Well Elution Plate | – | – | 2 | – | 2 |
Product Insert | 1 | 1 | 1 | 1 | 1 |
5 Layer Cell Factory One Wide Mouth And One Narrow Mouth
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
PRODUCT FEATURES
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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