DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.
DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.
(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl Succinimidyl Carbonate is a Succinimidyl ester/NHS-functionalized cyclooctyne derivative for addition of the cyclooctyne moiety into amine-containing compounds or biomolecules. The BCN group can react with azide-tagged biomolecules under copper free conditions.
(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl Succinimidyl Carbonate is a Succinimidyl ester/NHS-functionalized cyclooctyne derivative for addition of the cyclooctyne moiety into amine-containing compounds or biomolecules. The BCN group can react with azide-tagged biomolecules under copper free conditions.
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots |
| Applications | PCR, qPCR, southern bolt and virus detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples |
| Sample amount | Solid tissue : 1-10mg, Anticoagulant blood : 200μl |
| Yield | 1 – 15µg |
| Elution volume | ≥20μl |
| Time per run | 30 – 60 minutes |
| Liquid carrying volume per column | 750µl |
| Binding yield of column | 100µg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | IVD3018 |
| Purification Times | 100 |
| 2ml Collection Tubes | 2 x 100 |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer GW1 | 44 ml |
| Buffer GW2 | 50 ml |
| Proteinase K | 60 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer AE | 15 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.