4x15ml PRP Swing Rotor

• Format: 96-well microtiter plate (12 test strips of 8 wells)
• Standards: 0 | 0.03 | 0.10 | 0.2 | 2 ppb
• Incubation Time: 45 Minutes
• Compatible for use with US EPA Method 546

Description

The Cylindrospermopsin plate kit is a competitive enzyme-labeled immunoassay. The Cylindrospermopsin sample extract and calibrators are pipetted into the test wells followed by the Cylindrospermopsin antibody into the test wells to initiate the reaction. During the 30 minutes incubation period, Cylin-drospermopsin from the sample and Cylindrospermopsin antigen compete for binding to the Cylindrosper-mopsin antibody. The Cylindrospermopsin antibody is captured on the walls of the test well. Following this 30-minute incubation, the contents of the wells are removed and the wells are washed to remove any unbound Cylindrospermopsin and free Cylindrospermopsin antibody. After wash, 1X HRP-conjugated Antibody#2 is added for 30 minutes incubation. The wells are washed afterwards, and a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 15-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Cylindrospermopsin concentration of the samples is derived.

Format:

• • 96-well microtiter plate (12 test strips of 8 wells)
• • Standards: 0 | 0.03 | 0.10 | 0.2| 2 ppb
• • Incubation Time: 45 Minutes
• Compatible for use with US EPA Method 546

EPA 10-Day drinking water Health Advisories for Cylindrospermopsin: 

Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.

Do not Drink – 3.0 μg/L for school age children to adults.

Do Not Use – 20 μg/L

EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L.

Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.03 | 0.10 | 0.2 | 2 ppb
Incubation Time: 45 Minutes
Compatible for use with US EPA Method 546

Description

Attogene’s Mercury Lateral Flow Kit can be used for the screening of Mercury in water and food (fish) samples at 10 ppb in a laboratory setting.  Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.  The instructions include method for extraction from food samples.

Mercury contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Mercury ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases.   Long-term health consequences of drinking Mercury-contaminated food and water include brain, heart, kidney, lungs  and immune system problems for adults, and the physical and mental development delays in infants and children.  Attogene’s Mercury Lateral Flow test gives results conforming of 10ppb or greater.  Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 5 minutes.

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Applications for Fish and Shellfish possible:

Mercury Levels in Commercial Fish and Shellfish (1990-2012)

Fish with recognized high concentrations of Mercury:

• Tuna, Grouper, Mackerel, Weakfish, Bluefish, Sablefish, Halibut, Bass Chilean, Shark, Marlin, Tilefish,

Screening of Mercury in water samples at 10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 5 Minutes

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Eleven discrete fragments ranging from 50 bp to 1000 bp
• Higher intensity reference band at 500 bp

The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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PCR Ranger 100 bp DNA Ladder (Cat# 11300) – 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 50 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	1000	91
2	900	66
3	800	61
4	700	51
5	600	43
6	500	70
7	400	28
8	300	23
9	200	30
10	100	22
11	50	15

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.