This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
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| Kit Specifications – Spin Column | |
| Column Binding Capacity | 10 μg |
| Size of DNA Purified | 100 – 15,000 bp |
| Average Recovery | > 90% |
| Average Primer Removal | > 90% |
| Minimum Elution Volume | 30 μL |
| Time to Complete 10 Purifications | 15 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 14400 (50 preps) | Cat. 45700 (250 preps) | Cat. 24800 (192 preps) |
|---|---|---|---|
| Binding Buffer C | 30 mL | 5 x 30 mL | 3 x 30 mL |
| Wash Solution A | 12 mL | 2 x 20 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 30 mL | 2 x 15 mL |
| Spin Columns | 50 | 250 | – |
| Collection Tubes | 50 | 250 | – |
| 96-Well Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 2 |
Attogene’s Mercury Lateral Flow Kit can be used for the screening of Mercury in water samples at 10 ppb in the field.
Mercury contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Mercury ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking Mercury-contaminated food and water include brain, heart, kidney, lungs and immune system problems for adults, and the physical and mental development delays in infants and children. Attogene’s Mercury Lateral Flow test gives results conforming of 10ppb or greater. Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 5 minutes.
Applications for Fish and Shellfish possible:
Mercury Levels in Commercial Fish and Shellfish (1990-2012)
Samples requiring regulatory action should be confirmed.
Screening of Mercury in water samples at 10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 5 Minutes
This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels in the field.
This is a revolutionary product designed to make Lead testing in water safe, easy, and affordable. This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L). The kit can test water quality from various sources including faucets, wells (ground water), and home purification systems.
Standards and Regulations for Lead [ACCLPP 2012]
High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death. Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.
Sample method:
1. Take a first-draw sample
Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.
2. Take a flush sample
If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.
3. Take sequential samples
If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.
Screening of Lead in water samples 5-10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 15 Minutes