Usages:
For determination of total bacterial count .
Principle:
Tryptone provide carbon and nitrogen; yeast extract powder provides B vitamins; glucose to provide energy, agar is the solidifying agent.
Formulation(per liter):
Tryptone 5.0g
Yeast extract powder 2.5g
Glucose 1.0g
Agar 15.0g
Final pH 7.0 ± 0.2
How to use:
1.Suspend 23.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
Herpes Simplex Virus 1 (HSV-1) is a member of the herpes virus family, Herpesviridae. HSV-1 has a relatively large double-stranded DNA genome. HSVs are primarily transmitted by sexual intercourse, direct contact with lesions or perinatally. Most HSV positive cases are characterised by lesions on the skins and mucous membranes of the mouth and genitals. HSV infection can be either primary or a recurrence of a previous infection. More than 90% of the primary HSV infections are asymptomatic. Primary infection with HSV-1 can lead to gingivostomatitis, eczema herpeticum, keratoconjunctivitis and encephalitis. The primary symptoms of a secondary infection are skin lesions in the nose, mouth and genital regions. The infection is contagious, mainly during an epidemic.
HSV-1 TaqMan PCR Kit, 100 reactions
HSV-1 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM32650 (100 preps) | Cat. TM32610 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| HSV-1 Primer & Probe Mix | 280 μL | 280 μL |
| HSV-1 Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
