Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Specifications
Features | Specifications |
Concentration | 100 mg/ml |
Appearance | Suspension of black particles |
Surface functional group | Si-OH, Silanol |
Dispersibility | Polydisperse Amorphous |
Particle size | 1.5-5 μm |
Preservation conditions | Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth. |
Magnetic response speed | 15-30 seconds |
Settling velocity | >5 minutes |
High salt mediated binding | >2M guanidine isothiocyanate, DNA recovery up to 80% |
Alcohol mediated binding | 2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
DNase/RNase | Not detected |
DNA residue | <1 ppm |
Recommended application | Plasmid extraction, gel DNA recovery, genomic DNA extraction and RNA extraction. |
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
Ordering information
CAT.No. | Product Name | Package |
C14100 | MagPure Particles | 100 ml |
C14101 | MagPure Particles | 400 ml |
C14102 | MagPure Particles | 3 x 400 ml |
C14103 | MagPure Particles | 10 x 400 ml |
Features | MagPure Particles | MagPure Particles N | MagPure Particles G | MagPure Particles F | MagBind Particles |
Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
Concentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
Color | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
Magnetic response | 15-30s | ~60s | ~30s | 20s | 120s |
Settling Time (1ml) | >5min | >10min | >3min | >3min | >2h |
Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
Recommended Use | gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification | DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up | Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation | Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction | DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays |
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Discover Huankai’s GC Agar Base, designed for the selective cultivation and isolation of Neisseria gonorrhoeae and Neisseria meningitidis, ensuring reliable and accurate microbial detection.
Discover Huankai’s GC Agar Base, designed for the selective cultivation and isolation of Neisseria gonorrhoeae and Neisseria meningitidis, ensuring reliable and accurate microbial detection.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 200μl whole blood |
Applications | PCR, southern bolt and virus detection, etc |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
Sample amount | 200μl |
Elution volume | ≥50μl |
Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Contents | D631101 | D631102 | D631103 |
Purification Times | 48 | 96 | 480 |
MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
Proteinase K | 24 mg | 50 mg | 220 mg |
Protease Dissolve Buffer | 1.8 ml | 5 ml | 15 ml |
Buffer AL | 15 ml | 30 ml | 120 ml |
Buffer BD | 5 ml | 10 ml | 50 ml |
Buffer GW1 | 22 ml | 53 ml | 220 ml |
Elution Buffer | 15 ml | 30 ml | 100 ml |
Storage and Stability
Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage(up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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