| Clone | IHC005 |
| Source | Mouse Monoclonal |
| Positive Control | Tonsil |
| Dilution Range | 1:200 |
PAX-5 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. The PAX-5 gene encodes the B-cell lineage specific activator protein (BSAP), whose expression is limited to early stages of B-cell differentiation. Anti-PAX-5 is useful in differentiating between classic Hodgkin’s lymphoma versus multiple myeloma and solitary plasmacytoma, as the protein is expressed in mature and precursor B-cell non-Hodgkin’s lymphomas/leukemias while being absent from the other two conditions. Diffuse large B-cell lymphomas are positive for PAX-5, with the exception of those with terminal B-cell differentiation, and T-cell neoplasms do not stain with Anti-PAX-5.
Product Description
High-Sensitivity RNA Detection Reagent for Gene Expression Analysis
Product Description:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
Features:
Technical Parameters:
| Product Name | Technical Parameters |
|---|---|
| Nucleic Acid Extraction Reagent | Application: DNA Extract Form: Liquid Shelf Life: 12 Months Brand: Amp-future Bio Automated Nucleic Acid Extractor, DNA Extraction Kit, Automated Nucleic Acid Extractor |
Applications:
Amp-future Bio Nucleic Acid Extraction Reagent is a liquid reagent specially formulated for automated nucleic acid extraction. It is widely used in DNA/RNA extraction from various sample matrixes, such as blood, tissue, cells, bacteria, etc. It ensures high-quality nucleic acid extraction in a short period of time. The reagent is produced in China and has a shelf life of 12 months.
Amp-future Bio Nucleic Acid Extraction Reagent is suitable for use with the Automated Nucleic Acid Extractor. It is highly efficient and reliable, allowing for fast and accurate nucleic acid extraction. The reagent is also suitable for use with the DNA Extraction Kit, which makes it ideal for clinical laboratories. It provides superior nucleic acid extraction performance.
Amp-future Bio Nucleic Acid Extraction Reagent is an essential product for laboratories that need to extract nucleic acids from various sample matrixes. It is highly reliable and efficient, providing superior results in a short period of time. The reagent is also suitable for use with the Automated Nucleic Acid Extractor and the DNA Extraction Kit. It is a reliable and cost-effective product for laboratories that require high quality nucleic acid extraction.
Customization:
Customized Nucleic Acid Reagent Service
Amp-future bio provides custom nucleic acid reagent services to meet your specific needs. We specialize in the production of DNA/RNA extraction reagents for automated nucleic acid extraction, purification and other related applications.
Our products are suitable for DNA extraction, automated nucleic acid extraction, nucleic acid purification, and other related applications. We are confident that our products can meet the highest quality standards and provide the best performance. We are dedicated to providing the best customer service and technical support for our customers.
Support and Services:
Our team of experts provide the highest quality technical support and services for Nucleic Acid Reagents products.
Our technical support staff are available to assist you with any questions or troubleshooting related to the use of Nucleic Acid Reagents. Our team can provide guidance on the proper use and handling of our products, as well as any other technical questions. We offer technical support via phone, email, and online chat.
We also offer a variety of services to support our customers in the use of Nucleic Acid Reagents. These services include:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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| Kit Specifications | |
| Maximum Binding Capacity Per Column | Up to 50 μg RNA |
| Maximum Loading Volume Per Spin Column | 650 μL |
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications | 25 minutes |
| Maximum Amount of Starting Material | |
| Liver Tissue | 20 mg |
| Heart Tissue | 5 mg |
| Kidney Tissue | 20 mg |
| Brain Tissue | 25 mg |
| Lung Tissue | 20 mg |
| Spleen Tissue | 20 mg |
| Whole Blood | 100 µL |
| Hela Cells | 3 x 106 |
| CHO | 3 x 106 |
| Yeast | 1 x 108 cells |
| E.coli | 1 x 109 CFU/mL |
| Bacteria | 1 x 109 cells |
| Plasma/Serum | 200 µL |
| Fungi | 50 mg |
| Plant Tissue | 50 mg |
| Viruses | ≤100 µL of viral suspension |
| Average Yield | |
| HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg | |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 48300 (50 preps) | Cat. 48400 (100 preps) |
|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL |
| RNA Purification Columns | 50 | 100 |
| Genomic DNA Removal Columns | 50 | 100 |
| Collection Tubes | 100 | 200 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
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