Alkyne-ethyl-PEG1-t-Butyl ester has an alkyne group and a t-butyl protected carboxyl group. The alkyne reacts with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Thecarboxyl group group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Alkyne-ethyl-PEG1-t-Butyl ester has an alkyne group and a t-butyl protected carboxyl group. The alkyne reacts with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Thecarboxyl group group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture.
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 1mg plasmid DNA from 200ml bacterial culture |
| Applications | Enzyme digestion, sequencing, PCR and labeling, etc. |
| Purification method | Maxi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Conventional plasmid, plasmid less than 30KB |
| Sample amount | 100-200ml |
| Yield | 0.4-1mg |
| Elution volume | ≥500μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 20ml |
| Binding yield of column | 1mg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
| Contents | P101402 | P101403 |
| Purification Times | 10 Preps | 50 Preps |
| RNase A | 20 mg | 40 mg |
| Buffer P1 | 140 ml | 2 x 350 ml |
| Buffer P2 | 140 ml | 2 x 350 ml |
| Buffer LEN3 | 70 ml | 350 ml |
| Buffer GBT | 120 ml | 550 ml |
| Buffer PW1 | 60 ml | 300 ml |
| Buffer PW2* | 50 ml | 4 x 100 ml |
| Elution Buffer | 20 ml | 120 ml |
| HiPure DNA Maxi Columns III | 10 | 50 |
| Lysate Clear Maxi Syringe | 10 | 50 |
| 50 ml Collection Tubes | 20 | 100 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture.
Mycobacterium tuberculosis is a pathogenic bacterial species belonging to the genus Mycobacterium, and is the causative agent of tuberculosis. Tuberculosis (TB) is a multifaceted disease and challenging public health problem in both industrialized and developing countries. According to the WHO, 8.8 million active cases of TB are diagnosed each year and of these, almost 2 million die. Once thought to be under control or even close to extinction, TB infection levels are rising and the threat is compounded by new, virulent and drug-resistant strains. Although most cases occur in the developing world (22 countries accounting for 80% of all global cases), increasing population mobility combined with facility of transmission means that no country is immune from the resurgence of TB. TB control programs are currently facing a number of constraints. Worldwide, fewer than 25% of all tuberculosis cases are detected. Of utmost concern is the absence of a timely and accurate test for the diagnosis of mycobacterial disease. Early diagnosis is crucial for the prevention of further spread of the disease.
Mycobacterium tuberculosis TaqMan PCR Kit, 100 reactions
Mycobacterium tuberculosis TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM42150 (100 preps) | Cat. TM42110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| M. tuberculosis Primer & Probe Mix | 280 μL | 280 μL |
| M. tuberculosis Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |