Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Opentrons OT-2 Single-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for low to medium throughput workflows with high precision.
Opentrons OT-2 Single-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for low to medium throughput workflows with high precision.
Permagen’s XLE PCR-96 low volume bar magnet plate for PCR applications features an individual bar magnet for each row of wells, making it the perfect solution for hand pipetting as all of the pelleted beads will be to the same side in each row. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
A012424 (magnet plate only)
A012424KT (Includes top plate kit for 0.2 mL strips and tubes)
*only recommended for manual use as semi-, and non-skirted plates will not locate correctly into the nest but can be positioned against the magnet
Minimum – 5 µL
Maximum – 120 mL
Permagen’s XLE PCR-96 low volume bar magnet plate for PCR applications features an individual bar magnet for each row of wells, making it the perfect solution for hand pipetting as all of the pelleted beads will be to the same side in each row. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips