Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.
100 count, 200 uL max volume per well
Labware definition is available for immediate use in Opentron’s Labware Library
NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.
100 count, 200 uL max volume per well
Labware definition is available for immediate use in Opentron’s Labware Library
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue, cell |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Tissues, cells, lymphocytes and other clinical sample |
| Sample amount | Cells grown in suspension:3~5 x 106Animal tissue: 10~20mgPlant tissue: ≤100mg |
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water
Advantages
Kit Contents
| Contents | IVD3020 |
| Purification Times | 200 Preps |
| MagPure RNA Particles | 7 ml |
| DNase I | 4 x 600 µl |
| DNase Buffer | 80 ml |
| RTL Lysis Buffer | 150 ml |
| Buffer MCB* | 75 ml |
| Buffer MW1* | 110 ml |
| Buffer MW2* | 50 ml |
| RNase Free Water | 60 ml |
| Cat.No | Reagent | IVD3020-F-96 |
| DNase Buffer | 60 ml | |
| DNase I | 2 x 600 μl | |
| RTL Lysis Buffer | 80 ml | |
| Buffer MCB | 18 ml | |
| 96-Tip | 1 | |
| Sample plate (DW Plate) | 500μl Buffer MCB | 1 |
| Wash 1 Plate (DW Plate) | 700μl Buffer MW130μl MagPure RNA Partilces | 1 |
| DNase Plate | Empty | |
| Wash 2 Plate (DW Plate) | 700μl Buffer MW1 | 1 |
| Wash 3 Plate (DW Plate) | 900μl Buffer MW2 | 1 |
| Elution plate (DW Plate) | 80μl RNase Free Water | 1 |
| Cat.No | Reagent | IVD3020-TL-06 |
| Purification times | 96 Preps | |
| DNase I | 2 x 600 μl | |
| DNase Buffer | 60 ml | |
| RTL Lysis Buffer | 60 ml | |
| Buffer MCB | 40 ml | |
| 96-Tip | 12 PCS | |
| 2.0ml V-bottom plate | Row 1/7：500μl Buffer MCBRow 2/8：500μl Buffer MW1Row 3/9：emptyRow 4/10：30μl Magpure RNA Particles500μl Buffer MW2 Row 5/11：900μl Buffer MW2 Row 6/12：80μl RNase Free Water | 6 |
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.