This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
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| Kit Specifications – Spin Column | |
| Column Binding Capacity | 10 μg |
| Size of DNA Purified | 100 – 15,000 bp |
| Average Recovery | > 90% |
| Average Primer Removal | > 90% |
| Minimum Elution Volume | 30 μL |
| Time to Complete 10 Purifications | 15 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 14400 (50 preps) | Cat. 45700 (250 preps) | Cat. 24800 (192 preps) |
|---|---|---|---|
| Binding Buffer C | 30 mL | 5 x 30 mL | 3 x 30 mL |
| Wash Solution A | 12 mL | 2 x 20 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 30 mL | 2 x 15 mL |
| Spin Columns | 50 | 250 | – |
| Collection Tubes | 50 | 250 | – |
| 96-Well Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 2 |
t-Boc-N-Amido-PEG7-propargyl is a crosslinking reagent that enables the formation of triazole linkage with azides under the catalyzation of copper. The Boc-protected amine can be deprotected under mild acidic conditions. By introducing PEG chain into the molecule, the hydrophilicity can be greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG7-propargyl is a crosslinking reagent that enables the formation of triazole linkage with azides under the catalyzation of copper. The Boc-protected amine can be deprotected under mild acidic conditions. By introducing PEG chain into the molecule, the hydrophilicity can be greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Opentrons Heater-Shaker Module, compatible with both the OT-2 and Flex robots, provides on-deck heating and orbital shaking of microplates. The module can be heated to 95C, and can shake samples from 200 to 3000 rpm. It is compatible with a number of deep-well and 96-well plates. The Heater-Shaker can be used simultaneously with other Opentrons modules.
The Heater-Shaker GEN1 is compatible with the new Opentrons Flex Robot when paired with the Flex Heater-Shaker Caddy and Calibration Adapter. The caddy sits below the deck to enable below-deck cable routing and labware placed on modules remain closer to the deck surface. The calibration adapter is used to calibrate the location of the module. If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.
The Opentrons Heater-Shaker Module, compatible with both the OT-2 and Flex robots, provides on-deck heating and orbital shaking of microplates. The module can be heated to 95C, and can shake samples from 200 to 3000 rpm. It is compatible with a number of deep-well and 96-well plates. The Heater-Shaker can be used simultaneously with other Opentrons modules.
The Heater-Shaker GEN1 is compatible with the new Opentrons Flex Robot when paired with the Flex Heater-Shaker Caddy and Calibration Adapter. The caddy sits below the deck to enable below-deck cable routing and labware placed on modules remain closer to the deck surface. The calibration adapter is used to calibrate the location of the module. If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.