4x2x100ml Swing Buckets Rotor 

Name of Product

HAMA – Human Anti Mouse
Antibodies Detection Kit

Catalog Number

MQHM Z

Short Info

Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.

This product is only available in the EU!

Method/Platform

Lateral Flow

Range/Assay Sensivity

97%

Test Principle

The HAMA Detection Kit is an immunoassay designed for qualitative determination of Human Anti Mouse Antibodies in human serum.

HAMA from patient’s sample bind first to mouse antibodies which are conjugated to gold nanoparticles.

In a second step the HAMA will be caught by mouse IgG antibodies, coated on the Test Line (T) of the nitrocellulose membrane.

In the presence of the HAMA-conjugat-complex  a band becomes visible at the location of the Test Line (T).

The surplus of gold particles continues to migrate through the membrane and is captured at the Control Line (C) by specific antibodies.

Color’s intensitiy of the test line is directly proportional to the HAMA concentration in the sample.

Brief Instructions

Storage

2-8°C

Components

HAMA Test Unit, Chase Buffer, Evaluation Cards

5 tests
Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.

Tetra(3-methoxy-N-(prop-2-ynyl)propanamide) Methane is a 4-branched molecule with propargyl groups that can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Tetra(3-methoxy-N-(prop-2-ynyl)propanamide) Methane is a 4-branched molecule with propargyl groups that can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

Usages:
For isolation and enumeration of coagulase-positive staphylococci in foodstuffs.

Principle:
Tryptone, beef extract powder and yeast extract powder provides carbon and nitrogen sources, vitamins and growth factors; sodium pyruvate and glycine to stimulate the growth of Staphylococcus aureus; lithium chloride and potassium tellurite inhibit the non-staphylococcal microorganisms; containing lecithinase staphylococcal colonies produce degradation yolk make transparent circle, while the role of the lipase produced an opaque precipitate ring; coagulase-positive staphylococci can restore potassium tellurite and produce black colonies; agar is medium coagulant.

Formulation（per liter）:
Pancreatic Digest of Casein 10g
Beef Extract 5g
Yeast Extract 1g
Sodium Pyruvate 10g
Glycine 12g
Lithium Chloride 5g
Agar 20g
Final pH7.0 ± 0.2

How to use：
1.Suspend 63g in 950ML of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.

Quality control：
          　　

Item	The name and number of strain	Growth	Colony Color
1	Staphylococcus aureus ATCC6538	Good	black
2	Staphylococcus epidermidis CMCC (B) 26069	Good	Green-blue
3	Escherichia coli ATCC25922	Not growth	—

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle
*Supplement: 029190 Egg Yolk Tellurite Emulsion

250g