Introduction

HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 500 mg soil sample
Applications	RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Forest soil, grassland soil, mining area soil, sediment and other samples
Sample amount	500 mg
Elution volume	≥30μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – extraction of several samples can be completed in 60 minutes by column purification method
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be purified at the level of PG

Kit Contents

Contents	R418302	R418303
Purification Times	50 Preps	250 Preps
HiPure RNA Micro Columns	50	250
2ml Collection Tubes	100	500
gDNA Filter Column	50	250
2ml Beads Tubes	50	250
Buffer SOL	30 ml	150 ml
Buffer SDS	4 ml	15 ml
Buffer PHC	30 ml	150 ml
Buffer GDP	40 ml	150 ml
Buffer RW1	50 ml	200 ml
Buffer RW2 *	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability

Buffer PHC should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolvedbefore use. Make sure that all buffers are at room temperature when used.

Experiment Data

HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

DBCO-PEG4-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG4-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Cell Culture Flask T175

Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.

Volume: 25mL75mL175mL225mL

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Two kinds of product line up are providing.

For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.

For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.

• Large mouthed design makes easy operation of pipet or cell scraper. The surface of flask is uniform and smooth, hence the clear view can be obtained when microscopic observation.
• The hydrophobic filter cap can prevent invasion of fungi and bacteria without water absorb.
• Gamma radiation sterilization
• Non Byrogenic Nase/Bnase free

Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.

Volume: 25mL75mL175mL225mL