The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA from 0.25ml body fluids using column and MagZol LS reagent |
Applications | qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection |
Purification method | Mini spin column |
Purification technology | Silica technology, acidphenol / guanidine extraction technology (MagZol pretreatment technology) |
Process method | Manual (centrifugation or vacuum) |
Sample type | Blood, serum, plasma |
Sample amount | 250μl |
Elution volume | ≥20μl |
Liquid carrying volume per column | 800µl |
Binding yield of column | 100μg |
0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.
Advantages
Kit Contents
Contents | R416302 | R416303 |
Purification Times | 50 Preps | 250 Preps |
HiPure RNA Mini Columns | 50 | 250 |
2ml Collection Tubes | 50 | 2 x 125 |
MagZol LS Reagent | 60 ml | 270 ml |
Buffer RWC | 20 ml | 60 ml |
Buffer RW2* | 20 ml | 2 x 50 ml |
RNase Free Water | 10 ml | 30 ml |
Storage and Stability
MagZol LS Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.
Description
The DM4100 ExcelBand™ XL 25 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM4100 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
250 ~ 25,000 bp
Concentration
51.6 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
The DM4100 ExcelBand™ XL 25 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM4100 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
Propargyl-PEG1-t-butyl ester has a propargyl group and a t-butyl protected carboxyl group. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The t-butyl protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG1-t-butyl ester has a propargyl group and a t-butyl protected carboxyl group. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The t-butyl protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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