RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Figure 1 / 4
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Kit Specifications | |
Maximum Column Binding Capacity | 50 μg for RNA 20 μg for DNA 200 μg for protein |
Maximum Column Loading Volume | 650 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Size of DNA Purified | ≥ 30 kb |
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues | 5 x 106 cells 25 mg (for selected tissues) 100 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg |
Time to Complete 10 Purifications | 30 minutes |
Average Yield:HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells) | 15 μg RNA8 μg DNA150 μg protein |
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 47700 (50 preps) | Cat. 51600 (50 preps) | Cat. 51700 (96 preps) |
---|---|---|---|
Buffer SKP | 40 mL | 40 mL | – |
Lysis Buffer Q | – | – | 40 mL |
Wash Solution A | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL |
Elution Solution A | 6 mL | 6 mL | 20 mL |
Elution Buffer F | 15 mL | 15 mL | 2 x 15 mL |
Wash Solution C | 30 mL | 30 mL | 60 mL |
Binding Buffer A | 8 mL | 8 mL | 8 mL |
Elution Buffer C | 8 mL | 8 mL | 30 mL |
Protein Neutralizer | 4 mL | 4 mL | 4 mL |
Protein Loading Dye | 2 mL | 2 mL | 3 x 2 mL |
gDNA Purification Columns | 50 | – | – |
gDNA Purification Micro Columns | – | 50 | – |
gDNA Purification 96-Well Plate | – | – | 1 |
RNA/Protein Purification Columns | 50 | – | – |
RNA/Protein Purification Micro Columns | – | 50 | – |
RNA/Protein Purification 96-Well Plate | – | – | 1 |
Collection Tubes | 150 | 150 | – |
Collection Plate | – | – | 5 |
Elution Tubes (1.7 mL) | 150 | 150 | – |
Elution Plate | – | – | 3 |
Lysis Preparation Plate | – | – | 2 |
Adhesive Tape | – | – | 4 |
Product Insert | 1 | 1 | 1 |
K-GCROL
SKU: 700004292
70 assays (manual) / 700 assays (microplate)
Content: | 70 assays (manual) / 700 assays (microplate) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Glycerol |
Assay Format: | Spectrophotometer, Microplate |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Decrease |
Linear Range: | 0.8 to 35 µg of glycerol per assay |
Limit of Detection: | 0.34 mg/L |
Reaction Time (min): | ~ 5 min |
Application examples: | Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices, soft drinks, toothpaste, honey, tobacco, paper (and cardboard), cosmetics, pharmaceuticals, soap and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Methods based on this principle have been accepted by OIV and MEBAK |
The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Browse our wide range of alcohol assay kit products.
Advantages
The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
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