Character
Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.
Refrigeration Series
● Dual compressors,Secop special compressors,air cooling (water cooling system is optional)
● Refrigeration method:Cascade refrigeration method,high cooling efficiency,good reliability,using CFC-Free environmentally friendly refrigerant.
Silicone oil conducts heat,with uniform temperature and accuracy up to 0.1°C.Silicone oil has a wide temperature range,up to-100°C~+600°C.
● Shelf refrigeration system and cold trap refrigeration system are completely independent,without any switching,refrigeration temperature is lower and more stable.
Vacuum series
● Vacuum pump:German Pfeiffer anti-corrosion,anti-oil return function vacuum pump.(Vacuum pump can be selected according to user needs)
● Pumping rate:9m³/h~62m³/h,multiple specifications are optional,the ultimate vacuum can reach 2×10-3mbar,and the oil mist filter is standard.
● The vacuum pipelines are all high-vacuum stainless steel vacuum tubes, which can effectively prevent vacuum leakage and corrosion.
● The double anti-oil return device completely eliminates the damage and
equipment and samples.
● Automatic backfill air device,the system has a built-in High efficiency particulate air(HEPA)filter to ensure the complete cleanliness of backfill air.
● The vacuum stabilization system can stabilize the vacuum degree of the freeze-drying process through the micro-pass vacuum control valve to ensure that the samples will not be affected by excessive fluctuations in the vacuum degree.
● Condensed liquid collection and discharge system,automatically completes the collection and discharge of condensed liquid after freeze-drying.
Control System Characteristic
● Control:Automatic/Manual
● Can store at least 100 independent freeze-drying programs,each program contains 32 program segments (Can be
customized according to user needs),each program segment corresponds to a processing stage (pre-freezing,main
drying,post-drying).and Include the following segment values:Cycles,shelf temperature,vacuum and safety pressure
● All the equipment can be recorded in real time and the corresponding freeze-drying curves and data tables can be generated after the end.The records of all data and curves comply with the corresponding regulations and standards.
● It has hierarchical management authority,hierarchical management allocation authority and usage process tracking authority
● Remote System
O Fully support 2G/4G/GSM/Wi-Fivarious connection modes
o Users can remotely view all data on the device and operate it
o Technicians can remotely perform maintenance and fault detection on equipment,and can remotely guide and train users
o Can remotely upgrade and change the system of the equipment
Parameter
| Mode | MP2 | MP5 | MP8 | MP12 |
| Freeze-dried area | 0.24m | 0.48m² | 0.96m² | 1.45m² |
| Ice condenser capacity | 4kg | 9kg | 12kg | 18kg |
| Shelf temperature | 70℃~+80℃ | 65℃~+70℃ | ||
| Ice condenser temperature | 70 /-90℃ | 70/-90℃ | -70℃/-90℃ | -70℃/-85℃ |
| Cold trap volume | 9L | 15L | 18L | 30L |
| Chamber type | single | double | ||
| Max number of shelves | 2+1 | 5+1 | 8+1 | 12+1 |
| Shelf size | 275*400 mm | 275*400 mm | 365*465 mm | 365*465 mm |
| Shelf spacing | 100mm(adjustable) | 100mm(adjustable) | 70mm(adjustable) | 70mm(adjustable) |
| Sample quantity (4R Cillin bottle) | 558 | 1395 | 2630 | 4230 |
| Shelf heating and cooling method | Thermal conducting silicone oil medium(temperature resistance:-100℃~+600℃) | |||
| Chamber and shelf surface | Atomization and electrolytic surface treatment,no dead corner residue | |||
| Pipeline diameter | 中 8 9 m m | |||
| External valve port | 6/ | 12/ | 18/ | |
| Equipment power | 3.8kw | 5.5kw | 6.5kw | 8kw |
| Equipment size | 700*588*1600 mm | 1030*700*1630 mm | 1030*700*1850 mm | 1500*850*1900 mm |
| Capping mode | Hydraulic system gland device/no gland | Hydraulic pressure | ||
| Suitable clean room | suitable | |||
| Vacuum sensor type | PiraniVacuum sensor/capacitive vacuum sensor | |||
| Remote control and alarm | Support 2G/4G/GSM/Wi-Fi | |||
| Terminal judgment function | Pressure contrast method Pressure test/pressure contrast method | |||
| Freeze dryer front door cover | Stainless steel/Plexiglass | Stainless steel | ||
| Inert gas filling | (Optional)Sufficient amount and time for the program to run itself | |||
| User function customization | Can be customized and modified according to user needs | |||
| Sterilization method | HzOz/steam sterilization | |||
| Note:”/”optional.Pilot models can be customized according to user needs | ||||
Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.
Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit
For sample input volumes ranging from 5 mL to 10 mL.
Cell Culture Media Exosome Purification and RNA Isolation Midi Kit
For sample input volumes ranging from 10 mL to 20 mL.
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit
For sample input volumes ranging from 20 mL to 35 mL.
Figure 1 / 3
Click for expanded view
| Kit Specifications | |
| Sample Type | Cell-Free Cell Culture Media |
| Sample Volume Range | 10 mL to 20 mL |
| Size of RNA Purified | All sizes, including miRNA and small RNA (<200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 40-45 minutes |
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60ºC if any salt precipitation is observed.
| Component | Cat. 60700 (50 preps) | Cat. 60800 (25 preps) | Cat. 60900 (15 preps) |
|---|---|---|---|
| Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
| ExoC Buffer | 1.5 mL | 1.5 mL | 1.5 mL |
| ExoR Buffer | 12 mL | 12 mL | 12 mL |
| Lysis Buffer A | 20 mL | 20 mL | 20 mL |
| Lysis Additive B | 2 mL | 2 mL | 2 mL |
| Wash Solution A | 18 mL | 18 mL | 18 mL |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Mini Filter Spin Column | 50 | 25 | 15 |
| Mini Spin Columns | 50 | 25 | 15 |
| Collection Tubes | 50 | 25 | 15 |
| Elution tubes (1.7 mL) | 100 | 50 | 30 |
| Product Insert | 1 | 1 | 1 |
