Product Description
RNA Amplification Kit: Accurate Results, Superior Quality
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex;perform a homology search and bind the target homology domain,at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation,the polymerase also binds to the 3′ end of the primer, initiating chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit EXO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(Fluorescent type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Name of Product
Echinococcus granulosus – IgG ELISA
Catalog Number
AF 9350
Short Info
This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus granulosus (and Echinococcus multilocularis) in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Echinococcus granulosus Antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
12 x 8 strips (96 tests)
This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus granulosus (and Echinococcus multilocularis) in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.
Water RNA/DNA Purification Kit (Spin Column + Filters Kits)
These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water.
Water RNA/DNA Purification Kit (Spin Column)
This kit is sold without filters columns.
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| Kit Specifications | |
| Minimum Water Input | 10 mL |
| Maximum Water Input | 100 mL |
| Maximum Filter Column Loading Volume | 20 mL |
| Maximum Spin Column Loading Volume | 650 µL |
| Elution Volume | 100 µL |
| Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
| Component | Cat. 26400 (25 preps) | Cat. 26450 (25 preps) | Cat. 26480 (50 preps) |
|---|---|---|---|
| Lysis Buffer E | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution A | 18 mL | 18 mL | 38 mL |
| Enzyme Incubation Buffer B | 6 mL | 6 mL | 6 mL |
| Elution Buffer H | 6 mL | 6 mL | 6 mL |
| Mini Spin Columns | 25 | 25 | 50 |
| Filter Columns (0.22 μL) | 25 | – | – |
| Filter Columns (0.45 μL) | – | 25 | – |
| Bead Tubes | 25 | 25 | 50 |
| Collection Tubes | 25 | 25 | 50 |
| Elution Tubes (1.7 mL) | 25 | 25 | 50 |
| Product Insert | 1 | 1 | 1 |