Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kits provide fast and simple procedures for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.
AAV Purification Kit
Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. Up to 33X sample concentration.
AAV Purification Mini Kit
Each spin column is able to concentrate and purify AAV from 0.5-8 mL of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL). Preparation time for 4 samples is 1.5 hours, with 45 minutes of hands-on time.
AAV Purification Midi Kit
Each spin column is able to concentrate and purify AAV from 8 mL up to 45 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL). The kit may be used to purify up to 8 x 25 mL or 4 x 45 mL of samples using the included columns. Preparation time for 4 samples is approximately 2 to 2.5 hours, with 1.5 hours of hands on time.
AAV Purification Maxi Kit (Slurry Format)
Each spin column is able to concentrate and purify AAV from 45 mL to 90 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 200X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step. The kit may be used to purify up to 1 x 900 mL samples or 10 x 45-90 mL samples using the included columns. Preparation time for 1 x 900 mL sample is approximately 2.5 to 3.5 hours, with an optional concentration step requiring an additional 30 min.
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| Kit Specifications | |
| Resin Binding Capacity (total per kit) | At least 5 x 1010 AAV particles as determined by qPCR |
| AAV Vector Serotype | AAV6, AAV9 and others |
| Input Type | Cells, media |
| Input Volume (AAV supernatant) | 1 – 33.5 mL SN per prep (500 mL SN in total) |
| Input Volume (AAV cell pellet) | 1 mL cell pellet per prep (15 mL in total) |
| Time to Complete Purifications | 2.5 to 4.5 hours with 1 hour hands on time |
| In vivo transduction | Yes |
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 66100 (15 preps) | Cat. 63200 (20 preps) | Cat. 63300 (4-8 preps) | Cat. 63250 (1-10 preps) |
|---|---|---|---|---|
| Lysis Buffer S | 5.5 mL | 5.5 mL | 5.5 mL | 20 mL |
| DNAse I | – | 2 x 25 uL | 2 x 25 uL | 210 μL |
| RNAse A | – | 60 μL | 60 μL | 240 μL |
| HL-SAN Nuclease | 102 μL | – | – | – |
| Binding Buffer A | 20 mL | 4 mL | 4 mL | 2 x 8 mL |
| Purification Solution C | 60 mL | – | – | – |
| Purification Solution D | 130 mL | – | – | – |
| Wash Solution C | 2 x 130 mL | 60 mL | 60 mL | 3 x 60 mL |
| Slurry E | 12.5 mL | – | – | 2 x 14.5 mL |
| Elution Buffer O | 66 mL | 8.5 mL | 8.5 mL | 66 mL |
| Protein Neutralizer | 4 mL | 4 mL | 4 mL | 4 mL |
| Spin Columns | – | 20 | – | – |
| Mini Spin Columns | – | 20 | – | – |
| Midi Spin Columns (grey contents) with Collection Tubes | – | – | 8 | 10 |
| Midi Spin Columns (white contents) with Collection Tubes | – | – | 8 | – |
| Maxi Spin Columns (grey contents) with Collection Tubes | – | – | – | 10 |
| Maxi Spin Columns (white contents) with Collection Tubes | – | – | – | 10 |
| Collection Tubes | – | 40 | – | – |
| Elution tubes (1.7 mL) | 50 | 20 | – | – |
| Midi Elution tubes (15 mL) | – | – | 8 | 10 |
| Maxi Elution tubes (50 mL) | – | – | – | 10 |
| Product Insert | 1 | 1 | 1 | 1 |
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
For the detection of the SARS-CoV-2 variants with the VUI-21APR-01 and VUI-21APR-03 (India)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
TD5B Large Capacity Low Speed Centrifuge
Features:
1. Brushless motor with simpler construction, more reliable performance, longer life and quietly running.
2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.
4. Microprocessor control, TD5B with digital display which indicate the speed, time, RCF in operation, speed raising and reducing quick, operate simply.
5.10 kinds of accelerating and decelerating speed for your choice.
TD5B Technical Parameter：
| Max. Speed | 5000rpm |
| Max. RCF | 4730×g |
| Max. Capacity | 4×800ml |
| Time Range | 0~99min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 58 |
| Temperature | Normal |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | / |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 685×500×385mm |
| Net Weight(Kg) | 67KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TD5B
| Order No | Rotor | Max Speed(r/min) | Volume(ml) | Max RCF(×g) |
| 5B-1 | Swing Rotor | 4000 | 4×800ml | 3450 |
| 5B-2 | Swing Rotor | 4000 | 4×750ml | 3530 |
| 5B-3 | Swing Rotor | 4000 | 4×500ml | 3380 |
| 5B-4 | Swing Rotor | 4000 | 4×4×96(well) | 2940 |
| 5B-5 | Microplate rotor | 4000 | 2×4×96(well) | 3210 |
| 5B-6 | 2×3×48(well) | 2300 | ||
| 5B-7 | Swing rotor | 5000 | 4×1×50ml | 4730 |
| 4×1×100ml | 4730 | |||
| 4000 | 4×2×100ml | 3020 | ||
| 4000 | 4×2×50ml | 3200 | ||
| 4000 | 4×4×15ml | 3200 | ||
| 4000 | 4×6×15ml | 3200 | ||
| 5B-8 | Swing rotor | 4000 | 4×8×15ml | 3200 |
| 4000 | 4×12×5ml vacuum tube | 2480 | ||
| 4000 | 4×12×7ml vacuum tube | 2760 | ||
| 4000 | 4×12×10ml vacuum tube | 2880 | ||
| 4000 | 4×8×10/7/5ml vacuum tube | 2790 | ||
| 4000 | 4×6×10/7/5ml vacuum tube | 2790 | ||
| 4000 | 4×4×10/7/5ml vacuum tube | 2790 | ||
| 5B-9 | Swing rotor | 4000 | 4×24×5ml vacuum tube | 2480 |
| 4000 | 4×31×5ml Khan tubes | 2480 | ||
| 5B-10 | Swing rotor | 4000 | 4×24×7ml vacuum tube | 3140 |
| 4000 | 4×31×7ml Khan tubes | 3140 | ||
| 4000 | 4×30×7ml vacuum tube | 3140 | ||
| 4000 | 4×30×5ml vacuum tube | 3140 | ||
| 4000 | 4×40×7ml vacuum tube | 3270 | ||
| 4000 | 4×18×10ml vacuum tube | 3140 | ||
| 5B-11 | Swing rotor | 4000 | Dairy bottle | 3830 |
| 5B-12 | Fixed rotor | 5000 | 6×15ml | 2540 |
| 5B-13 | 5000 | 12×15ml | 3080 | |
| 5B-14 | 5000 | 24×15ml | 3500 | |
| 5B-15 | 5000 | 30×15ml | 3830 | |
| 5B-16 | 5000 | 4×50ml | 2520 | |
| 5B-17 | 5000 | 6×50ml | 2850 | |
| 5B-18 | 5000 | 12×50ml | 3860 | |
| 5B-19 | 4000 | 24×50ml | 2970 | |
| 5B-20 | 5000 | 4×100ml | 2630 | |
| 5B-21 | 5000 | 6×100ml | 3130 | |
| 5B-22 | 4000 | 12×100ml | 2970 |
Adapter for 800ml and 500ml bucket
| VolumeCup | 5ml | 7ml | 10ml | 15ml | 50ml |
| 800ml（Round Cup） | 23pcs/cup | 23 | 19 | 14 | 5 |
| 500ml（Round Cup） | 18pcs/cup | 18 | 14 | 9 | 3 |
| 500ml（Square Cup） | 20pcs/cup | 20 | 16 | 12 | 5 |
TD5B large capacity centrifuge max capacity can be reached to 4x800ml, can with different adapters for small tubes, 50ml, 15ml, 10ml, 7ml, 5ml, 2ml and so on.