Legionella sp. TaqMan PCR Kit Dx, 24 reactions
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Storage Conditions
The Legionella sp. TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. DxTM64400 (24 rxns) |
|---|---|
| MDx TaqMan 2X PCR Master Mix | 550 μL |
| Legionella sp. Primer & Probe Mix Dx | 2 x 70 μL |
| Legionella sp. Positive Control Dx – 200,000 copies/μL | 50 μL |
| Nuclease-Free Water | 2 x 1.25 mL |
| Product Insert | 1 |
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.2g), purification of DNA from PCR or enzymatic reaction solution solution |
| Applications | PCR, NGS, labeling, ligation and enzyme digestion, etc. |
| Purification method | Micro spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Agarose gel, PCR products, enzyme products |
| Sample amount | Agarose gel: ≤200mgDNA products: ≤300µl (≤5µg) |
| Recovery | 100-5000bp：85%, 5-10kb：60-70% |
| Elution volume | ≥10μl |
| Time per run | ≤30 minutes（1-24 samples） |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D211002 | D211003 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer GDP | 60 ml | 125 ml |
| Buffer DW1 | 40 ml | 90 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elution Buffer | 20 ml | 30 ml |
| HiPure DNA Micro Columns | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.
