4x4x10ml Swing Buckets Rotor

Overview

Thin polyester heat sealing film which is easily pierceable with autosampler needles/ABI^® 3730. The seal is suitable for PCR, qPCR and optical applications.

• Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
• Our ClearASeal Pierce heat sealing film is an optically clear polyester backed film, forming a pierceable seal to polypropylene, polyethylene, polystyrene and cyclic olefin copolymer (COC) plates
• The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays
• Its pierceability renders it useful for automation and for use on needle, capillary and tip based liquid handling systems
• It is effective on the ABI^®3730 capillary sequencer, removing the need for the use of expensive septa mats
• The ClearASeal Pierce forms a complete seal to a plate enabling moderately low and high temperature uses, including PCR when using a pressurized heated lid thermal cycler
• It demonstrates a moderate solvent resistance and can be utilized for short term compound storage
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Thin polyester heat sealing film which is easily pierceable with autosampler needles/ABI® 3730. The seal is suitable for PCR, qPCR and optical applications.

Introduction

Usages:
For selective isolation of Gram-negative bacteria, especially for Shigella and Salmonella.

Principle:
Yeast extract powder provide nitrogen, vitamins, growth factors; sodium chloride to maintain osmotic equilibrium ferric ammonium citrate of iron salts to produce a black iron sulfide; agar as medium coagulant; phenol red as pH indicator.

Formulation（per liter）:
Xylose 3.50g
L-Lysine 5.00g
Lactose 7.50g
Sucrose 7.50 g
Sodium Chloride 5.00 g
Yeast Extract 3.00g
Sodium Desoxycholate 2.50g
Sodium Thiosulphate 6.80g
Fe-Ammonium Citrate 0.80g
Phenol Red 0.08g
Agar 13.50
Final PH 7.4±0.2    

How to use:
1.Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave ,cool to 50℃ and pour into sterile petri dishes.
2.Dilluted and treated samples.

Quality control:
Quality control strains were inoculated ,and cultured at 36 ± 1 ℃ for 24h ,results show as follows:
strain name              strain code            growth           feature
Arizona bacteria       CMCC (B) 47001          good            black colonies
Salmonella typhimurium CMCC (B) 50115          good            black colonies
Streptococcus faecalis    CMCC32223            prohibited         —        

Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.

Specifications: 250g/bottle

250g

OverView

To accommodate the need for high quality enzymes for isothermal amplification ArcticZymes developed the IsoPol® series of polymerases. For isothermal amplification at lower temperatures (37°C and below) we offer two enzymes, IsoPol® DNA Polymerase and IsoPol® SD⁺. Both enzymes exhibit excellent processivity and high strand displacement activity, having 5’-3’ polymerase activity while lacking both 3’-5’and 5’-3’ exonuclease activity.

IsoPol® SD⁺ is an engineered version of IsoPol® DNA Polymerase with even stronger strand displacement and higher salt tolerance.

Figures

Properties

Quality Control

ArcticZymes is dedicated to the quality of our products. IsoPol® polymerases are is manufactured at our ISO 13485 certified facility in Norway.

To accommodate the need for high quality enzymes for isothermal amplification ArcticZymes developed the IsoPol® series of polymerases. For isothermal amplification at lower temperatures (37°C and below) we offer two enzymes, IsoPol® DNA Polymerase and IsoPol® SD+. Both enzymes exhibit excellent processivity and high strand displacement activity, having 5’-3’ polymerase activity while lacking both 3’-5’and 5’-3’ exonuclease activity.