Overview

• Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
• Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
• Can process challenging samples such as mastitic milk
• Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
• Fast and efficient spin-column format

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example.  The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.​

Details

Supporting Data

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*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.

Details

Specifications

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.

Advantages

• Fast – several samples can be extracted in 40 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• Good repeatability – silica technology can obtain ideal results every time
• High recovery – DNA can be recovered at the level of PG
• Broad spectrum – it can deal with a variety of bacteria, including Gram-positive bacteria that are difficult to disrupt
• Sufficient components – this kit has carried lysozyme, protease K, RNase A and glass beads

Kit Contents

Storage and Stability

Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.

Introduction

Usages:
Yeast extract promotes growth of a wide variety of microorganisms, including yeasts and moulds.

Technical specification:
Total nitrogen(TN)—————————≥10.0%
Amino nitrogen(AN) ————————≥4.5%
Sulphuric ash———————————-≤15.0%
Loss on drying——————————–≤6.0%
Chlorides————————————–≤0.5%
PH(5% solution)——————————7.0±0.5
Proteoses—————————————positive
Tryptophan———————————— positive
Nitrites—————————————–negative


Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.

Specifications: 500g/bottle; 10kg /bag

500g/bottle;10KG/bag