4x4x10ml Swing Buckets Rotor

endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Convenient optimized on-column DNase treatment using Norgen’s RNA Purification Kits
• Also includes protocol for digestion in-solution followed by RNA Clean-Up
• Guaranteed RNase-Free
• Includes Enzyme Incubation Buffer
• Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)

Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

Details

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

Propargyl-PEG10-acid is a propargyl PEG linker with a terminal carboxylic acid. The terminal carboxylic acid forms amide bonds with primary amines; activators (EDC or HATU) will be needed. The propargyl group can react with azide-bearing compounds to form stable triazole bonds. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG10-acid is a propargyl PEG linker with a terminal carboxylic acid. The terminal carboxylic acid forms amide bonds with primary amines; activators (EDC or HATU) will be needed. The propargyl group can react with azide-bearing compounds to form stable triazole bonds. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.