Human Immunodeficiency Virus (HIV) is a retrovirus which destroys essential cells in the human immune system, such as CD4+ lymphocytes (a type of T cell), macrophages and dendritic cells. Infection with HIV therefore leads to an impaired immune system and increased susceptibility to opportunistic cancers and infections. Due to the mutating nature of HIV and damage to the T cells, the immune system is unable to fight off infections and disease. Without treatment, HIV can progress to Acquired Immunodeficiency Syndrome (AIDS). The rate of HIV progression to AIDS depends on a number of different factors including viral, host and environmental factors. However, with adherence to antiretroviral therapy (ART), many individuals with HIV will never progress to AIDS, having near normal life expectancy. Transmission of HIV is through direct contact with infected bodily fluids, either sexually; by the sharing of blood-contaminated needles or instruments; maternally through childbirth or breastfeeding; or via blood transfusion/organ transplant.
HIV TaqMan RT-PCR Kit, 100 reactions
HIV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM33750 (100 preps) | Cat. TM33710 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| HIV Primer & Probe Mix | 280 μL | 280 μL |
| HIV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
ProbeSure Multiplex Master Mix is an enhanced version of ProbeSure Master Mix, formulated to enable users to analyse up to four targets in one reaction well. For example, two bi-allelic SNPs or one reference gene and a further three genes of interest.
Users will require a plate reader capable of reading FAM, HEX, ATTO 550, ATTO 647N and ATTO 633 (the wavelengths of each of these can be found in our ProbeSure Multiplex Master Mix User Guide). ProbeSure Multiplex Master Mix is supplied at 2x concentration for convenience and is supplied with the ATTO 633 normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ATTO 633.
GL20 Technical Parameter：
| Max. Speed | 21000rpm |
| Max. RCF | 30910×g |
| Max. Capacity | 4×800ml |
| Time Range | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 60 |
| Temperature | -20℃~40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 680×540×830mm |
| Net Weight(Kg) | 170KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for GL20
| Order No | Rotor | Rotor Max Speed(r/min) | Volume(ml) | RCF(×g) |
| G20-1 | Swing Rotor | 4000 | 4×800ml | 3450 |
| G20-2 | Microplate rotor | 4000 | 4×4×96 well | 2940 |
| G20-3 | Microplate rotor | 4000 | 2×4×96 well | 3210 |
| G20-4 | Microplate rotor | 4000 | 2×3×48 well | 2300 |
| G20-5 | Swing rotor | 4000 | 4×40×7ml vacuum tube | 3270 |
| G20-6 | 4000 | 4×40×5ml vacuum tube | 2890 | |
| G20-7 | 4000 | 4×28×10ml vacuum tube | 3130 | |
| G20-8 | Swing Rotor | 4000 | Fat bottle | 3830 |
| G20-9 | Fixed Rotor | 16000 | 4×8PCR | 15760 |
| G20-10 | Fixed Rotor | 15000 | 6×8PCR | 21420 |
| G20-11 | Fixed Rotor | 16000 | 8×8PCR | 17480 |
| G20-12 | Fixed Rotor | 15000 | 12×8PCR | 22930 |
| G20-13 | Fixed Rotor | 15000 | 40×0.5ml | 22920 |
| G20-14 | Fixed Rotor | 21000 | 12×1.5ml | 30910 |
| G20-15 | Fixed Rotor | 16000 | 24×1.5ml | 23440 |
| G20-16 | Fixed Rotor | 14000 | 30×1.5ml | 20800 |
| G20-17 | Fixed Rotor | 13000 | 48×1.5ml | 17930 |
| G20-18 | Fixed Rotor | 16000 | 16×5ml | 22020 |
| G20-19 | Fixed Rotor | 16000 | 6×10ml | 21500 |
| G20-20 | Fixed Rotor | 15000 | 12×10ml | 22680 |
| G20-21 | Fixed Rotor | 13000 | 16×10ml | 19490 |
| G20-22 | Fixed Rotor | 13000 | 8×15ml | 17790 |
| G20-23 | Fixed Rotor | 11000 | 12×15ml | 14330 |
| G20-24 | Fixed Rotor | 5000 | 24×15ml | 3500 |
| G20-25 | Fixed Rotor | 5000 | 30×15ml | 3830 |
| G20-26 | Fixed Rotor | 14000 | 6×30ml | 19060 |
| G20-27 | Fixed Rotor | 13000 | 6×50ml(conical) | 18840 |
| G20-28 | Fixed Rotor | 13000 | 6×50ml(round) | 18730 |
| G20-29 | Fixed Rotor | 5000 | 12×50ml | 3860 |
| G20-30 | Fixed Rotor | 4000 | 24×50ml | 2970 |
| G20-31 | Fixed Rotor | 13000 | 4×85ml | 18940 |
| G20-32 | Fixed Rotor | 12000 | 4×100ml | 14850 |
| G20-33 | Fixed Rotor | 10000 | 6×100ml | 11380 |
| G20-34 | Fixed Rotor | 4000 | 12×100ml | 2970 |
| G20-35 | Fixed Rotor | 12000 | 24 capillaries | 15800 |
| G20-36 | Swing Rotor | 15000 | 4×5ml | 19920 |
| G20-37 | Vertical rotor | 16000 | 16×5ml | 16450 |
| G20-38 | Vertical rotor | 14000 | 8×30ml | 19750 |
Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.
7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.