N-(Propargyl-PEG2)-DBCO-PEG3-acid facilitates the creation of triazole linkages with azide-containing compounds using Click Chemistry. The carboxylic group can react with amine-containing entities using coupling agents like HATU for further conjugation.
N-(Propargyl-PEG2)-DBCO-PEG3-acid facilitates the creation of triazole linkages with azide-containing compounds using Click Chemistry. The carboxylic group can react with amine-containing entities using coupling agents like HATU for further conjugation.
Description
The PM5200 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5200 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM5200 ExcelBand™ 3-color Pre-Stained Protein Ladder Broad Range resolves 15 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
The PM5200 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5200 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines such as CHO, E. coli, E1A, HEK293, Vero, NS0 and Baculovirus. For quantification of residual DNA, we recommend using the resDNASEQ Residual DNA Quantitation kit as described in the resDNASEQ Residual DNA Quantitation kit User Guide. To ensure accurate quantitative results, each sample in triplicate and perform a single PCR reaction for each extraction.
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Only three steps will be need for Sample Preparation,and the total time of Sample preparation is about 30 minites.
The good Recovery rate of Sample Preparation.
All components of the Sample Preparation Kit can be stored at room temperature.
Note: Price not include shipment & duty, contact us to get full quote.
The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines. The operation is easier and the higher nucleic acid yield is guaranteed at the same time.
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