4x4x10ml Swing Buckets Rotor

RNA into DNA and PCR in one step? Then, this enzyme will simplify PCR analysis from RNA templates reducing labor and time. RT-KTQ2 was evolved from the thermostable KlenTaq DNA polymerase with no significant reverse transcriptase activity. Four mutations ensure that the variant is reverse transcriptase active and even PCR active, while maintaining the thermostability. This allows to perform reactions at high temperatures minimizing problems encountered with strong secondary structures in RNA that melt at elevated temperatures. For further information refer to the original publication.

Available upon request and for R&D use only – Contact Us

RT-KTQ2 DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.

The enzyme can also be used for real-time cycling, when adding a suitable dye.

RNA into DNA and PCR in one step? Then, this enzyme will simplify PCR analysis from RNA templates reducing labor and time. RT-KTQ2 was evolved from the thermostable KlenTaq DNA polymerase with no significant reverse transcriptase activity. Four mutations ensure that the variant is reverse transcriptase active and even PCR active, while maintaining the thermostability. This allows to perform reactions at high temperatures minimizing problems encountered with strong secondary structures in RNA that melt at elevated temperatures. For further information refer to the original publication.

K-BETA3

SKU: 700004268

100 assays per kit

The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.

See more of our amylase assay kits and other kit products for measurement of enzyme activities.

Advantages

• Very cost effective 
• All reagents stable for > 2 years as supplied 
• Only enzymatic kit available 
• Very specific 
• Simple format 
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.

See more of our amylase assay kits and other kit products for measurement of enzyme activities

Overview

• Extract high quality & quantity total RNA including miRNA 
• Isolate from a wide variety of animal tissues 
• No phenol step required – isolate all RNA in one fraction
• Compatible with tissues stored in RNAlater® or Trizol®  
• Convenient & fast spin column format
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc.  Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins, and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Details

Supporting Data

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Storage Conditions and Product Stability
The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date shipment.