Overview

• Isolate all sizes of circulating DNA from plasma and serum samples
• Isolate Viral and Bacterial DNA
• Versatile plasma and serum input volumes
• Concentrate circulating DNA into small elution volumes
• Different elution strategies depending on the downstream application
• Isolate inhibitor-free circulating DNA for any application including PCR, qPCR, methylation-sensitive PCR
• Compatible with Streck Cell-Free DNA BCT® Tubes
• Isolate DNA with high quality and quantity from milk
• Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.​​

Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.

Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.

Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.

Details

Supporting Data

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Kit Specifications
Minimum Plasma/Serum Input	2 mL
Maximum Plasma/Serum Input	10 mL
Size of DNA Purified	≥ 50 bp
Elution Volume	100 μL
Time to Complete 10 Purifications	15-20 minutes
Average Yields	Variable depending on specimen

Note: Do not exceed the recommended sample input volume of 10 mL.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 50600 (50 preps)	Cat. 51200 (20 preps)	Cat. 51300 (10 preps)
Lysis Buffer D	65 mL	125 mL	3 x 125 mL
Slurry A1	–	6 mL	–
Slurry A2	12 mL	–	12 mL
Binding Buffer B	20 mL	6 mL	20 mL
Binding Buffer C	–	–	30 mL
Wash Solution A	2 x 38 mL	2 x 38 mL	3 x 38 mL
Elution Buffer B	8 mL	8 mL	15 mL
Mini Filter Spin Columns	50	20	–
Midi Filter Spin Columns	–	–	10
Midi Collection and Elution Tubes	–	–	20
Collection Tubes	50	40	10
Spin Columns	–	20	10
Elution Tubes (1.7 mL)	50	40	10
Product Insert	1	1	1

Introduction

The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate
Applications	Enzyme digestion, sequencing, PCR, labeling, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	1-1.5ml（x96）
Yield	1-15µg/1ml
Elution volume	≥70μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	70µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – it takes only 60 minutes to complete the isolation
• High yield – up to 1mg plasmid can be binded in one column
• Economy – high cost performance

Kit Contents

Contents	P100601	P100602	P100603
Purification Times	1 x 96 Preps	4 x 96 Preps	20 x 96 Preps
RNase A	5 mg	20 mg	100 mg
Buffer P1	30 ml	120 ml	600 ml
Buffer P2	30 ml	120 ml	600 ml
Buffer P3	40 ml	180 ml	800 ml
Buffer PW1	100 ml	500 ml	2 x 1000 ml
Buffer PW2	50 ml	2 x 100 ml	4 x 200 ml
Elution Buffer	150 ml	60 ml	300 ml
Lysate Clear Plate	1	4	20
HiPure DNA Plate	1	4	20
1.6 ml Collection Plate	1	4	20
0.5ml Elute Plate	1	4	20

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

Name	CAT NO	Features	Sampleamount	Elutionvolume	Yield
HiPure Plasmid Mini Kit	P1001	Classic, Regular application	1-5ml LB	30-50μl	5–35µg/5ml
HiPure Plasmid Mini Kit II	P1002	Classic, Regular application	5-15ml LB	75-100μl	20-80µg/15ml
HiPure Plasmid Plus 96 Kit	P1006	Classic, Regular application	1-1.5ml LB	70-150μl	1-15μg/1ml
HiPure Fastfilter Plasmid Midi Kit	P1013	Fast	30-50ml LB	0.3-0.8ml	50–250µg/50ml
HiPure Fastfilter Plasmid Maxi Kit	P1014	Fast	100-200ml LB	0.5-1.5ml	0.4–1mg/200ml
Low endotoxin/Endotoxin-free Plasmid
HiPure Plasmid EF Maxi Kit	P1156	Classic method, General for all kinds of vectors	100-200ml LB	≥0.7ml	200–1500µg
HiPure Plasmid EF Mini Kit	P1154	Recommend for low copy vector, Thoroughly remove RNA	5-15ml LB	≥60μl	10–80µg
HiPure Plasmid EF 96 Kit	P1157	Recommend for low copy vector, Thoroughly remove RNA	1-5ml LBx96	≥75μl	30µg
HiPure Plasmid EF Midi Kit	P1231	Recommend for High copy vector, Low elution volume, High concentration	25-50ml LB	≥100μl	10-500μg
HiPure Plasmid EF Mega Kit	P1116	Recommend for High copy vector(>3μg/ml)	500ml LB	≥2ml	1-10mg

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here

The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Introduction

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.

Details

Specifications

Features	Specifications
Main FunctionsC	Co-isolation total RNA and DNA from FFPE tissue
Applications	RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor and pipetting workstation
Sample type	FFPE slice, FFPE puncture sample, embedded tissue
Sample amount	No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area.

Principle

FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.

Advantages

• Post digestion sorting – higher DNA and RNA yields
• Economy – the price is much lower than imported reagents

Kit Contents

Contents	IVD3026
Purification Times	200 Preps
MagBind Particles	9.0 ml
Proteinase K	180 mg
Protease Dissolve Buffer	10 ml
Buffer DPS	150 ml
Buffer FRL	40 ml
Buffer ATL	40 ml
Buffer AL	80 ml
Buffer BXW1*	110 ml
RNase Free Water	30 ml

Storage and Stability

Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.