Norgen’s EBV TaqMan PCR Lyophilized Kit is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s EBV TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
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| Component | Cat. TM41050L (100 preps) | Cat. TM41010L (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix (Lyo) | 4 x 350 μL | – |
| EBV Primer & Probe Mix (Lyo) | 280 μL | 280 μL |
| EBV Positive Control (Lyo) | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 3 x 1.25 mL | 1 x 1.25 mL |
| Product Insert | 1 | 1 |
Bacillus cereus is a rod-shaped, gram-positive bacterium. Some strains of B. cereus cause foodpoisoning and other diseases such as keratitis. B. cereus grows at a wide range of temperature, from 4°C to 37°C. In fact, many foodborne illnesses caused by B. cereus are a consequence of improperly cooked or improperly stored food, including dairy products and meats. The majority of food poisoning by B. cereus could be divided into two types of symptoms – the emetic type and the diarrheal type, both of which are caused by the enterotoxin produced by the bacteria.
Bacillus cereus TaqMan PCR Kit,
100 reactions
Bacillus cereus TaqMan PCR Probe/Primer Set and Controls,
100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM36950 (100 preps) | Cat. TM36910 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Bacillus cereus Primer & Probe Mix | 280 μL | 280 μL |
| Bacillus cereus Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
This kit provides for rapid spin column isolation and purification of total RNA and genomic DNA simultaneously from a single plant sample without splitting the lysate. Norgen’s plant lysis solution is highly robust and effective over a wide range of plants including challenging samples. The total RNA and genomic DNA are both column purified in under 30 minutes. Since RNA and DNA are isolated without splitting the lysate, variability and inconsistent results are reduced. All sizes of RNA including microRNA are recovered without the need for phenol. Optional on-column DNase and RNase treatments provide flexibility to isolate DNA-free RNA or RNA-free DNA respectively. Isolated nucleic acids are of a high quality and yield, and are ready for downstream use including PCR, qPCR, RT-PCR, qRT-PCR, sequencing and more.
Background
It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. This is of great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.
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| Kit Specifications | |
| Column Binding Capacity | 50 μg for RNA 15 μg for genomic DNA |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells | 100 mg 5 x 106 |
| Time to Complete 10 Purifications | 30 minutes |
| Average Yields* Peach Leaves (100 mg) | 40 μg RNA, 5 μg gDNA |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. 24400 (50 preps) |
|---|---|
| Lysis Buffer M | 40 mL |
| Binding Buffer I | 7 mL |
| Solution WN | 18 mL |
| Wash Solution A | 38 mL |
| Elution Buffer E | 20 mL |
| Enzyme Incubation Buffer B | 6 mL |
| Filter Columns | 50 |
| Spin Columns | 50 |
| Collection Tubes | 100 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |