Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
For the detection of the SARS-CoV-2 (20J/501Y.V3 Brazil)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
The Opentrons Heater-Shaker Module, compatible with both the OT-2 and Flex robots, provides on-deck heating and orbital shaking of microplates. The module can be heated to 95C, and can shake samples from 200 to 3000 rpm. It is compatible with a number of deep-well and 96-well plates. The Heater-Shaker can be used simultaneously with other Opentrons modules.
The Heater-Shaker GEN1 is compatible with the new Opentrons Flex Robot when paired with the Flex Heater-Shaker Caddy and Calibration Adapter. The caddy sits below the deck to enable below-deck cable routing and labware placed on modules remain closer to the deck surface. The calibration adapter is used to calibrate the location of the module. If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.
The Opentrons Heater-Shaker Module, compatible with both the OT-2 and Flex robots, provides on-deck heating and orbital shaking of microplates. The module can be heated to 95C, and can shake samples from 200 to 3000 rpm. It is compatible with a number of deep-well and 96-well plates. The Heater-Shaker can be used simultaneously with other Opentrons modules.
The Heater-Shaker GEN1 is compatible with the new Opentrons Flex Robot when paired with the Flex Heater-Shaker Caddy and Calibration Adapter. The caddy sits below the deck to enable below-deck cable routing and labware placed on modules remain closer to the deck surface. The calibration adapter is used to calibrate the location of the module. If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.
Specifications
| Features | Specifications |
| Main Functions | Extract Pathogen RNA/DNA from 0.5ml plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for mNGS application, remove host background nucleic acid. |
| Applications | RT-PCR，Real Time PCR, biochip analysis, NGS |
| Products | Pathogen DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | low/cell-free samples such as plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution |
| Sample amount | 0.5 ml |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Kit Contents
| Contents | R667200B | R6672-02B |
| Purification Times | 24 Preps | 96 Preps |
| 2ml Bead Tubes | 24 | 96 |
| Proteinase K | 12 mg | 50 mg |
| Protease Dissolve Buffer | 1.8 ml | 3 ml |
| Buffer SDS (20%) | 1.8 ml | 8 ml |
| MagBind Particles | 0.6 ml | 2.5 ml |
| Buffer MLB | 15 ml | 60 ml |
| Buffer MW1* | 13 ml | 44 ml |
| Buffer MW2* | 6 ml | 50 ml |
| Buffer AVE | 5 ml | 30 ml |
Storage and Stability
MagBind Particles and Proteinase K Solution should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.