Norgen’s Total Nucleic Acid Preservation Tubes are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months.
Swab samples collected and preserved using Norgen’s Total Nucleic Acid Preservation Tubes are compatible with most commercially available DNA and RNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA and RNA isolation kits and reagents, including Norgen’s Total RNA Purification Kit (Cat# 17200) and Norgen’s Saliva DNA Isolation Kit (Cat# RU45400). For purification of total nucleic acids please refer to the supplementary protocol provided with Norgen’s Total RNA Purification Kit (Cat#17200).
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| Kit Specifications | |
| Stability of DNA at Room Temperature | 4 months |
| Stability of RNA at Room Temperature | 2 months |
Storage Conditions and Product Stability
The Total Nucleic Acid Preservation Tubes can be stored at room temperature for up 1 year without any reduction in kit performance. The collect before date is indicated on the tube label and the kit box label.
| Kit Components | Cat. 69200 |
|---|---|
| Total Nucleic Acid Preservation Tubes | 50 |
| Product Insert | 1 |
More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. HPV 16 has been considered as a high-risk cancer associated HPV type. The low-risk HPV type 6 has been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA.
HPV 6/16 TaqMan PCR Kit, 100 reactions
HPV 6/16 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | TaqMan Probe Cat. TM42050 (100 preps) | TaqMan Probe/Primer and Control Set Cat. TM42010 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 µL | – |
| HPV 6/16 Primer & Probe Mix | 280 µL | 280 µL |
| HPV 6/16 Positive Control | 150 µL | 150 µL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Description
The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.
The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications
Features
Applications
Storage
-20°C for 24 months
The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.
The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications
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