4×4×96well Microplate Frame Type Rotor

Product Description

High Sensitivity Amp-Future DNA Isothermal Amplification Kit NFO, -20°C Storage, 48 Tests/Box, ICE Shipping

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Propargyl-PEG7-NHS ester contains a propargyl group and an NHS ester. As an amine reactive reagent, this product can be used for derivatizing peptides, antibodies, amine coated surfaces etc. In the presence of copper catalyst, propargyl group reacts with azide to yield a stable triazole linkage. The PEG units help increase the hydrophilicity of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG7-NHS ester contains a propargyl group and an NHS ester. As an amine reactive reagent, this product can be used for derivatizing peptides, antibodies, amine coated surfaces etc. In the presence of copper catalyst, propargyl group reacts with azide to yield a stable triazole linkage. The PEG units help increase the hydrophilicity of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples
• Small urine and CSF input ranging from as low as 0.5 mL to 1 mL
• No phenol extractions
• Very sensitive and linear down to a few cells without the need for carrier RNA
• Bind and elute all RNA irrespective of size or GC content, without bias
• Rapid and convenient spin column protocol
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses.

Details

Supporting Data

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Kit Specifications
Volume of Urine Processed	0.5 – 1 mL
Size of RNA Purified	All sizes, including < 200 nt
Time to Complete 10 Purifications	< 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 29000 (25 preps)
Lysis Buffer A	2 x 20 mL
Wash Solution A	18 mL
Elution Solution A	6 mL
Mini Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1