4x4x96well Microplate Rotor

Detail

Other Products

α-Amylase Assay Kit (Ceralpha Method)

Product Info

Document

Product Info

Document

[QP5510] Q-PAGE™ TGN Precast Gel (Midi, 12 wells, 4-15%), 10 gels

Product Info

Document

Product Info

Document

Plasma/Serum Circulating DNA Purification Kits (Slurry Format)

Product Info

Document

Product Info

Overview

Isolate all sizes of circulating DNA from plasma and serum samples
Isolate Viral and Bacterial DNA
Versatile plasma and serum input volumes
Concentrate circulating DNA into small elution volumes
Different elution strategies depending on the downstream application
Isolate inhibitor-free circulating DNA for any application including PCR, qPCR, methylation-sensitive PCR
Compatible with Streck Cell-Free DNA BCT® Tubes
Isolate DNA with high quality and quantity from milk
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.

Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.

Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.

Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.

Details

Supporting Data

Figure 1 / 10

Figure 1. Isolation of Circulating DNA from Different Plasma Volumes

Figure 2. Isolation of Viral DNA from Plasma

Figure 3. Reproducible Recovery of Circulating DNA

Figure 4. Reproducible Recovery of Circulating DNA

Figure 5. Detection of Human 5S Gene from Plasma

Figure 6. Determination of Rate of Inhibition in Plasma DNA Samples

Figure 7. Detection of Human 5S and S15 Genes

Figure 8. Detection of Spiked Viral DNA from 5 mL of Plasma

Figure 9. Scalability of the Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)

Figure 10. Determination of the Rate of Inhibition Present in Plasma DNA Samples

Click for expanded view

Kit Specifications
Minimum Plasma/Serum Input	50 μL
Maximum Plasma/Serum Input	400 μL
Minimum Elution Volume	100 μL
Time to Complete Purifications	< 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 50600 (50 preps)	Cat. 51200 (20 preps)	Cat. 51300 (10 preps)
Lysis Buffer D	65 mL	125 mL	3 x 125 mL
Slurry A1	–	6 mL	–
Slurry A2	12 mL	–	12 mL
Binding Buffer B	20 mL	6 mL	20 mL
Binding Buffer C	–	–	30 mL
Wash Solution A	2 x 38 mL	2 x 38 mL	3 x 38 mL
Elution Buffer B	8 mL	8 mL	15 mL
Mini Filter Spin Columns	50	20	–
Midi Filter Spin Columns	–	–	10
Midi Collection and Elution Tubes	–	–	20
Collection Tubes	50	40	10
Spin Columns	–	20	10
Elution Tubes (1.7 mL)	50	40	10
Product Insert	1	1	1

Document

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	α-Amylase
Assay Format:	Spectrophotometer, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	400
Signal Response:	Increase
Limit of Detection:	0.003 U/g
Reproducibility (%):	~ 3%
Total Assay Time:	~ 30 min
Application examples:	Cereal flours, fermentation broths and other materials.
Method recognition:	AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018)

4x4x96well Microplate Rotor

Detail

Other Products

α-Amylase Assay Kit (Ceralpha Method)

[QP5510] Q-PAGE™ TGN Precast Gel (Midi, 12 wells, 4-15%), 10 gels

Plasma/Serum Circulating DNA Purification Kits (Slurry Format)

Overview

Details

Supporting Data

A3P Scientific

A3P Scientific Head Office

Follow us