K-FUCOSE
SKU: 700004288
100 assays (manual) / 1000 assays (microplate) / 1020 assays (auto-analyser)
| Content: | 100 assays (manual) / 1000 assays (microplate) / 1020 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | L-Fucose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.5 to 100 µg of L-fucose per assay |
| Limit of Detection: | 0.68 mg/L |
| Reaction Time (min): | ~ 10 min |
| Application examples: | L-Fucose is present as the main component in fucoidan (a marine polysaccharide), foods, pharmaceuticals and other materials (e.g. biological samples, etc.). |
| Method recognition: | Novel method |
The L-Fucose test kit is a simple, rapid and reliable method, for the measurement and analysis of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our full range of monosaccharide and disaccharide assay kits.
Advantages
The L-Fucose test kit is a simple, rapid and reliable method, for the measurement and analysis of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.
Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
Oligo purification can also be performed using spin column-based technology. The oligo size limitation for recovery is around 20 nt, as oligos under 20 nt have a very low recovery rate.
We have developed Magnetic Beads (Short Oligo Purification) Kit for short oligo purification. Our proprietary bead technology enables the recovery of oligos as short as 6 nt. 80% of the 6 nt oligos and 90% of the > 8 nt oligos can be recovered. The reagent also effectively removes impurities and unwanted components such as salts, proteins, dNTPs, detergents, and other contaminants. The magnetic bead reagents are RNase free, and can be used for both DNA and RNA applications.
Recovery rates of of short oligos. Oligos with different sizes were used as input. BioDynami Short Oligo Quantification Kit (Cat. # 40046) was used to quantify the recovery rates with modifications.
Features
Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
Gly-PEG3-endo-BCN is a click chemistry reagent. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Gly-PEG3-endo-BCN is a click chemistry reagent. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.