Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is the etiological agent of the sexually transmitted infection (STI) gonorrhoea, which globally causes an estimated 60 million new cases of gonococcal disease annually. It is second only to Chlamydia trachomatis as the most reported notifiable sexually transmitted disease. Infections with N. gonorrhoeae are primarily restricted to the mucus membranes of the endocervix, urethra, rectum, and pharynx. In females, gonorrhoea is a major cause of pelvic inflammatory disease and may lead to tubal infertility, ectopic pregnancy, and chronic pelvic pain, whereas in males it primarily causes urethritis. Importantly, these infections may often be asymptomatic, thereby contributing to further transmission and maintenance of the disease within populations.
Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard is prepared from pelleted bacteria grown on culture plates using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Neisseria gonorrhoeae.
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Volume Provided – 250 µL
DNA Quantity – 2 x 104 copies per µL
Storage Conditions
Upon receipt, store Norgen’s Neisseria gonorrhoeae Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20oC or lower.
Biotin is extensively used in combination with streptavidin in biological assays. Our biotin detection kits have multiple applications in detecting biotin attachment and efficiency of conjugation during biotinylation processes. These kits are perfect for understanding the efficiency of the reaction and free biotin in your solutions that can interfear with Streptavidin-biotin interactions.
Attogene offers three kits to detect and quantify biotin using three different technologies including Lateral Flow, ELISA and Chemical. these kits are accurate and sensitive compared with common instrumental analysis.
Can be used to calculate the amount of biotin in liquid samples including biological samples including biotinylated antibody, free biotin in solution, foods, plasma and serum as needed. Also, can be used to quantify the % biotinylation following standard biotinylation reactions.
EL2021_EL: ELISA Biotinylation Quantification Kit
EL2021_EZ: TNBSA Biotinylation Quantification Kit
EL2021_LF: Lateral Flow kit Biotinylation Detection Kit
Highly Sensitive, rapid, robust screening kit for Biotin
Useful for detecting and quantifying biotin in biological samples
Adaptable for detecting biotin in food samples
Usages:
For cultivating of bacteria.And sterility test of drugs and biological products.
Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; glucose carbon source; dipotassium hydrogen phosphate as a buffering agent.
Formulation（per liter）:
Pancreatic Digest of Casein 17g
Papaic Digest of Soybean 3g
Sodium chloride 5g
Dipotassium hydrogen phosphate 2.5g
Glucose Monohydrate 2.5g
Final pH 7.3±0.2
How to use：
1.Suspend 30g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g