4x4x96well Microplate Rotor

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions

Description 

The ExcelDye™ 6× DNA Loading Dye (Orange) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration. The Orange G migrates at approximately 30 bp on a standard 2% TAE agarose gel (50 bp on 1% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition 

• 0.15% Orange G 
• 10 mM Tris-HCl (pH 8.0) 
• 60% glycerol 
• 60 mM EDTA

Storage 

4°C for 12 months
-20°C for 36 months

The ExcelDye™ 6× DNA Loading Dye (Orange) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration. The Orange G migrates at approximately 30 bp on a standard 2% TAE agarose gel (50 bp on 1% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Description 

The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase – an RNA dependent DNA polymerase capable of generating first strand cDNA using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. The ExcelRT™ Reverse Transcriptase is able to routinely synthesize first strand cDNA >8 kb at 37~50°C.

Features

• High yield
• Thermostable, up to 50°C, during first strand synthesis
• High processivity, generating cDNA up to 8 kb
• Reduced RNase H ribonuclease activity

Application

• Generation of first strand cDNA from total RNA or mRNA.
• Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.

Storage

-20°C for 24 months

High yield

Thermostable, up to 50°C, during first strand synthesis

High sensitivity

Contents

ComponentVolumeExcelRT™ Reverse Transcriptase (200 U/μl) 100 μl5X RT Buffer1 ml0.1 M DTT   500 μl

Storage Buffer

20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol 

5X RT buffer

250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl and 15 mM MgCl₂

Storage

-20°C for 24 months

The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase – an RNA dependent DNA polymerase capable of generating first strand cDNA using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. The ExcelRT™ Reverse Transcriptase is able to routinely synthesize first strand cDNA >8 kb at 37~50°C.