• All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
  + Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback). 
  + Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
  + Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.   
  + Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried. Can be freeze-dried by us. Learn MoreFor research use and further manufacturing. Designed and manufactured under ISO13485.

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Exemplary tetraplex PCR assay

Detection of three specific pathogen targets and an internal control. Total input DNA per reaction was 10^6 (red), 10^5 (yellow), 10^4 (blue), 10^3 (green), 10^2 (purple), 10 (light blue) and 0 copies (grey) and 8000 copies/reaction for the internal control (shown in the CY5 plot).

5x Multiplex – robust PCR perforemance for a wide range of qPCR application

PlexTaq 5x qPCR Multiplex Master Mix contains all components necessary for rapid, sensitive and reproducible quantification of DNA and cDNA. An engineered DNA polymerase and an optimized buffer including ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents aptamer prevents false amplification and provides a fast start function. 

PlexTaq®´s formulation allows to use it also for direct PCRs from crude samples.

All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!

+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
+ Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
+ Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
+ Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.

Introduction

This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from tissue, cell
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor, pipetting workstation
Sample type	Tissues, cells, lymphocytes and other clinical sample
Sample amount	Cells grown in suspension:3~5 x 106Animal tissue: 10~20mgPlant tissue: ≤100mg

Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water

Advantages

• High purity – OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – extraction without manual participation, saving time and effort
• Universal – suitable for various clinical samples

Kit Contents

Contents	IVD3020
Purification Times	200 Preps
MagPure RNA Particles	7 ml
DNase I	4 x 600 µl
DNase Buffer	80 ml
RTL Lysis Buffer	150 ml
Buffer MCB*	75 ml
Buffer MW1*	110 ml
Buffer MW2*	50 ml
RNase Free Water	60 ml

Cat.No	Reagent	IVD3020-F-96
DNase Buffer		60 ml
DNase I		2 x 600 μl
RTL Lysis Buffer		80 ml
Buffer MCB		18 ml
96-Tip		1
Sample plate (DW Plate)	500μl Buffer MCB	1
Wash 1 Plate (DW Plate)	700μl Buffer MW130μl MagPure RNA Partilces	1
DNase Plate	Empty
Wash 2 Plate (DW Plate)	700μl Buffer MW1	1
Wash 3 Plate (DW Plate)	900μl Buffer MW2	1
Elution plate (DW Plate)	80μl RNase Free Water	1

Cat.No	Reagent	IVD3020-TL-06
Purification times		96 Preps
DNase I		2 x 600 μl
DNase Buffer		60 ml
RTL Lysis Buffer		60 ml
Buffer MCB		40 ml
96-Tip		12 PCS
2.0ml V-bottom plate	Row 1/7：500μl Buffer MCBRow 2/8：500μl Buffer MW1Row 3/9：emptyRow 4/10：30μl Magpure RNA Particles500μl Buffer MW2 Row 5/11：900μl Buffer MW2 Row 6/12：80μl RNase Free Water	6

Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.