Usages:
Yeast extract promotes growth of a wide variety of microorganisms, including yeasts and moulds.
Technical specification:
Total nitrogen(TN)—————————≥10.0%
Amino nitrogen(AN) ————————≥4.5%
Sulphuric ash———————————-≤15.0%
Loss on drying——————————–≤6.0%
Chlorides————————————–≤0.5%
PH(5% solution)——————————7.0±0.5
Proteoses—————————————positive
Tryptophan———————————— positive
Nitrites—————————————–negative
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Specifications: 500g/bottle; 10kg /bag
500g/bottle;10KG/bag
African Swine Fever Virus (ASFV) is a widespread disease which infects members of the pig family(Suidae). Anumberoftick species are believed to be the vector for the disease,as well as being transmitted by raw pork and pig excrement [1]. After firstly being identified in Kenya in 1921, ASFV became endemic in sub-Saharan Africa, with regular outbreaks being reported across Europe, Asia and South America throughout the century [2]. More recently the virus was introduced in Georgia and spread throughout the region, as well as mass outbreaks occurring in China in 2018 [3].
ASFVistheonlymemberoftheAsfaridaefamily.ItisalargeenvelopeddoublestrandedDNA virus of icosahedral morphology with an average diameter of 200nm and isolates contain genomes between 170-190Kbp encoding for up to 167 open reading frames [2]. The morphology of ASFV consist of several concentric domains. An inner core contains the nucleoid coated with a thick protein layered core shell, which is surrounded by an inner lipid envelope , all of which is encompassed by the capsid [2]. ASFV begins its replication cycle in the nucleus of infected cells before moving to the cytoplasm where the majority of the replication takes place [2]. Gene transcription is highly regulated, with distinct classes of mRNA identified to accumulate at early, intermediate and late transcripts of the virus [2]. The disease induces acute haemorrhagic disease within its hosts, causing high fevers and skin haemorrhages, with death often occurring within ten days of clinical symptoms appearing [4].
References: 1: The Centre for Food Security and Public Health (2015), African Swine Fever. 2: Galindo, I. and Alonso, C., 2017. African swine fever virus: a review. Viruses, 9(5), p.103. 3: Zhou, X., Li, N., Luo, Y., Liu, Y., Miao, F., Chen, T., Zhang, S., Cao, P., Li, X., Tian, K. and Qiu, H.J., 2018. Emergence of African swine fever in China, 2018. Transboundary and emerging diseases, 65(6), pp.1482-1484. 4: Gallardo, C., Ademun, A.R., Nieto, R., Nantima, N., Arias, M., Martín, E., Pelayo, V. and Bishop, R.P., 2011. Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007. African Journal of biotechnology, 10(17), pp.3488-3497.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
50 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
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