Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.
Vibrio cholerae TaqMan PCR Kit, 100 reactions
Vibrio cholerae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM38550 (100 preps) | Cat. TM38510 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| V. cholerae Primer & Probe Mix | 280 μL | 280 μL |
| V. cholerae Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
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| Kit Specifications | |
| Column Binding Capacity | 50 µg |
| Maximum Column Loading Volume | 650 µL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 1 x 1010 pfu/mL enriched phages |
| Average Yield* | 3-15 µg DNA from 106-1010 pfu/mL of enriched phages |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 46800 (50 preps) | Cat. 46850 (100 preps) |
|---|---|---|
| Lysis Buffer B | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 8 mL |
| Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
DL6MB large capacity refrigerated centrifuge
Features:
1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.
2. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
3. Digital display which indicates the program, speed, time,RCF, temperature.
4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.
5. There are 6 kinds of rotors for your choice.
6. Electric lid lock, compact design, super speed and imbalance protection.
7. The centrifuge body is made of high-quality steel, safe and reliable.
DL6MB Technical Parameter：
| Max. Speed | 6000rpm |
| Max. RCF | 6600*g |
| Max. Capacity | 6×1000ml(6*500ml blood bag） |
| Time Range | 0~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 55 |
| Temperature | -20-40 degrees |
| Acc/Dcc | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V 50HZ/60HZ |
| Size (W x D x Hmm) | 800×675×830mm |
| Net Weight(Kg) | 310KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for DL6MB
| Order No. | Rotor No. | Max Speed (rpm) | Max Volume(ml) | Max.RCF（×g） |
| No30298 | Swing Rotor | 4200 | 6×1000ml | 5100 |
| NO30221 | Angle Rotor | 6000 | 4×300ml | 5390 |
| NO30222 | Angle Rotor | 6000 | 6×300ml | 5660 |
| NO30223 | Angle Rotor | 6000 | 6×500ml | 6600 |
Widely used in the field of blood bank, pharmaceutical factory and laboratory.