These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Figure 1 / 7
Click for expanded view
Kit Specifications – Spin Column | |
Column Binding Capacity | 50 μg |
Maximum Column Loading Volume | 650 μL |
Maximum Amount of Starting Material:Plant TissuesFungi (wet weight) | 100 mg 100 mg |
Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) | 18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
Plants | Plants (Cont’d) | Fungi |
Tomato | Turnip | Aspergillus niger |
Grape | Chinese Cabbage | Mucor racemosus |
Peach | Radish | Cladosporium cladosporioides |
Plum | Komatsuna | Fusarium oxysporum |
Pine Needles | Apricot | Penicillium sp. |
Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
Strawberry | Hydrangea | Pichia sp. |
Legumes | Fig | Rhizopus oryzae |
Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
Sorghum grass | Cherry | Fusarium sp. |
Tobacco | Saintpaulia | |
Arabidopsis | Lotus | |
Lichen | Carrot | |
Corn seed | Hansen | |
Sunflower seed | Pistachio | |
Olive seed | ||
Soybean seed |
Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
---|---|---|---|---|---|
Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL | 2 x 60 mL | 60 mL | 2 x 60 mL |
Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL | 25 mL | 7 mL | 25 mL |
Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL | 55 mL | 18 mL | 55 mL |
Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
Magnetic Bead Suspension | – | – | – | 4 x 1.1 mL | 2 x 8.5 mL |
Filter Columns | 50 | 250 | – | – | – |
Spin Columns | 50 | 250 | – | – | – |
Collection Tubes | 100 | 500 | – | – | – |
96-Well Plate | – | – | 2 | – | 2 |
96-Well Collection Plate | – | – | 2 | – | – |
Adhesive Tape | – | – | 4 | – | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | – | 50 | – |
96-Well Elution Plate | – | – | 2 | – | 2 |
Product Insert | 1 | 1 | 1 | 1 | 1 |
Name of Product
Lactobacillus/Pediococcus-Screen
Catalog Number
MGScLP
Short Info
This test discovers beer germs in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow tests.
Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.
The resulting PCR products carry the labels of the incorporated primers.
In a second part of the test, the created PCR products are detected by a lateral flow Test Strip. A “molecular sandwich” is formed and becomes visible as a line on the test Strip.
Brief Instructions
The PCR reagents and the samples are prepared.
After the addition of the sample to the PCR reagents, the PCR is started.
The resulting PCR products are detected by a simple lateral flow test
Storage
-15 to -20°C
Components
PCR-Polymerase Master-Mix, PCR-Primermix, Positive Kit-Control, PCR-Water
Clone | IHC412 |
Source | Mouse Monoclonal |
Positive Control | Colon |
Dilution Range | 1:200 |
Postmeiotic Segregation Increased 2 (PMS2) is a DNA repair protein involved in mismatch repair. Mutations and deficiencies in the PMS2 gene have been linked to microsatellite instability, and malignancies such as hereditary nonpolyposis colorectal cancer and endometrial cancer. Expression levels of the PMS2 protein may be useful as a screening tool for Lynch syndrome after a colorectal cancer diagnosis. Anti-PMS2 is recommended to be used as part of a panel along with antibodies against MLH1, MSH2, and MSH6.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map