4x600ml Swing Rotor 

DBCO-C5-NHS ester is an amine-reactive compound, which can be used to modify an amine-containing molecule in organic media. This reagent isn’t soluble in aqueous media. The extended 5-carbon atom spacer arm improves solubility in commonly used organic solvents including dichloromethane, chloroform, THF, and ethyl acetate, and it also improves derivatization efficiency and stability of conjugates. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.

DBCO-C5-NHS ester is an amine-reactive compound, which can be used to modify an amine-containing molecule in organic media. This reagent isn’t soluble in aqueous media. The extended 5-carbon atom spacer arm improves solubility in commonly used organic solvents including dichloromethane, chloroform, THF, and ethyl acetate, and it also improves derivatization efficiency and stability of conjugates. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.

Description 

AccuBand™ 100 bp DNA Ladder II is composed of 10 individual DNA fragments, presenting 1k, 900, 800, 700 600, 500, 400, 300, 200 and 100 bp sharp bands respectively. This product contains 1 enhanced band (500 bp) for easy identification of bands. AccuBand™ 100 bp DNA Ladder II is ready-to-use, containing loading buffer with dual color tracking dyes (orange G and Xylene cyanol FF). AccuBand™ 100 bp DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 100 bp to 1 kb, either in agarose gel or in polyacrylamide gel electrophoresis. 

Features

• Sharp bands
• Suitable for polyacrylamide gel electrophoresis
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 1,000 bp 

Concentration 

50 µg/ 500 µl 

Recommended loading volume 

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months 

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Isolates all sizes of exosomal and extracellular vesicle RNA, including microRNA.
• Bind and elute all RNA irrespective of size or GC content, without bias.
• No phenol extractions.
• No Proteinase K treatment.
• No carrier RNA.
• Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
• Purify high-quality RNA in 15-20 minutes.
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.

The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.