• Screening of Cylindrospermopsin in water samples at or above 30ppt
• Format: 15 tests (5 samples at 2 dilutions/5 controls)
• 5 – 10X Sample Dilution Buffer
• 5 – 1X Sample Dilution Buffer
• 5- Negative Control
• 10- Fixed Volume Pipettes

Description

Attogene’ s Cylindrospermopsin Lateral Flow Kit can be used to detect Cylindrospermopsin in source water samples.

Format: Rapid-Water – Run Time: 30 Minutes, enough to run two samples (undiluted and a 10 fold dilution) and negative control.

Cylindrospermopsin (CYN) is a potent cyanotoxin synthesized by select species of cyanobacteria, prominently including Cylindrospermopsin raciborskii. It belongs to the tricyclic alkaloid class, exhibiting a molecular weight of approximately 415 Da. Structurally, cylindrospermopsin features an uracil ring fused with a hydantoin moiety, alongside a guanidino group, attributes that render it highly soluble and polar in aqueous environments.

Cylindrospermopsin is notorious for its profound toxicity towards aquatic organisms and its potential threat to human health through exposure via contaminated water and food sources. Consequently, rigorous monitoring protocols are essential in regions prone to cyanobacterial blooms, where cylindrospermopsin can accumulate in freshwater reservoirs and other aquatic habitats. In recognition of these risks, regulatory bodies such as the United States Environmental Protection Agency (EPA) have implemented an action level guideline. As of 2019, EPA 10-day drinking water health advisory for cylindrospermopsin recommended a threshold of 0.7 parts per billion (ppb), or 700 parts per trillion (ppt) for children under the age of six, and 3 parts per billion, 3000 parts per trillion for anyone older, to effectively manage cylindrospermopsin levels. This precautionary measure aims to uphold both environmental sustainability and public health integrity by minimizing exposure risks.  The EPA has also drafted a human health recreational water quality criterion to protect human health at 8,000ppt.

EPA 10-Day drinking water Health Advisories for Cylindrospermopsin: 

Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions

Do not Drink – 3.0 μg/L for school age children to adults

Do Not Use – 20 μg/L

EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L

Screening of Cylindrospermopsin in water samples at or above 30ppt

Format: 15 tests (5 samples at 2 dilutions/5 controls)

5 – 10X Sample Dilution Buffer

5 – 1X Sample Dilution Buffer

5- Negative Control

10- Fixed Volume Pipettes

Introduction

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	0.2-0.6ml
Elution volume	≥30μl
Time per run	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labour

Kit Contents

Contents	IVD5432
Purification Times	200
MagPure Particles G	14 ml
Carrier RNA	310 μg
Proteinase K	180 mg
Protease Dissolve Buffer	10 ml
Buffer MLK	250 ml
Buffer MAW1	250 ml
Buffer MW2*	2 x 50 ml
Elution Buffer	60 ml

Contents	IVD5432-F-96A	IVD5432-F-96B	IVD5432-F-96C
Sample amount	200～350μl	400~700μl
Carrier RNA	110 μg	110 μg
Proteinase K	50 mg	100 mg
Protease Dissolve Buffer	5 ml	6 ml
Elution Buffer	15 ml	15 ml
Tip	1	1
Sample Plate A	500μl Buffer MLK	500μl Buffer MLK
Sample Plate B	/	500μl Buffer MLK
Wash Plate 1	700μl Buffer MAW1	700μl Buffer MAW1
Wash Plate 2	25μl Buffer MPG2700μl Buffer MW2	25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3	700μl Buffer MW2	700μl Buffer MW2
Elution Plate	/	/

Contents	IVD5432-TL-06A	IVD5432-TL-06B	IVD5432-TL-06C
Sample amount	300~350μl	600~700μl	900~1050μL
Carrier RNA	310 μg	310 μg	310 μg
Proteinase K	50 mg	100 mg	150 mg
Protease Dissolve Buffer	6 ml	6 ml	10 ml
Elution Buffer	15 ml	15 ml	15 ml
DA-Tip	12	12	12
Row 1/7	600μl Buffer MLK	600μl Buffer MLK	600μl Buffer MLK
Row 2/8	/	600μl Buffer MLK	600μl Buffer MLK
Row 3/9	600μl Buffer MAW1	600μl Buffer MAW1	600μl Buffer MLK
Row 4/10	20μl Buffer MPG2600μl Buffer MW2	20μl Buffer MPG2600μl Buffer MW2	30μl Buffer MPG2900μl Buffer MAW1
Row 5/11	600μl Buffer MW2	600μl Buffer MW2	900μl Buffer MW2
Row 6/12	/	/	/

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details