K-LMAL-116A
SKU: 700004311
| Content: | (K-LMAL-58A) 58 assays (manual) / 580 assays (microplate) (K-LMAL-116A) 116 assays (manual) / 1160 assays (microplate) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | L-Malic Acid |
| Assay Format: | Spectrophotometer, Microplate |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.5 to 30 µg of L-malic acid per assay |
| Limit of Detection: | 0.25 mg/L |
| Reaction Time (min): | ~ 3 min |
| Application examples: | Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by AOAC, EEC, EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN, NBN, ISO and MEBAK |
The K-LMAL-58A pack size has been discontinued (read more)
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Need other assay kits? View our full list of organic acid assay kits.
Advantages
These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.
Blood DNA Isolation Midi Kit
Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.
Blood DNA Isolation Maxi Kit
This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.
Blood DNA Isolation 96-Well Kit (High Throughput)
This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.
Blood DNA Isolation Kit (Magnetic Bead System)
Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications | |
| Minimum Blood Input | 20 µL |
| Maximum Blood Input | 200 µL |
| Column Binding Capacity | > 50 µg |
| Average Yield (200 µL of blood) | 4-12 µg* |
| Time to Complete 10 Purifications | 30 minutes |
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 46300 (50 preps) | Cat. 46380 (100 preps) | Cat. 51400 (20 preps) | Cat. 31200 (12 preps) | Cat. 46350 (192 preps) | Cat. 59800 (50 preps) | Cat. 62600 (192 preps) |
|---|---|---|---|---|---|---|---|
| Lysis Buffer B | 20 mL | 2 x 20 mL | 2 x 40 mL | 2 x 110 mL | 2 x 40 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
| Solution WN | 18 mL | 2 x 18 mL | 55 mL | 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 2 x 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 30 mL | 2 x 30 mL | 30 mL | 30 mL | 2 x 30 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
| Proteinase K in Storage Buffer | 1.2 mL | 2 x 1.2 mL | 4.4 mL | 8 mL | 4.5 mL | 1.2 mL | 4 mL |
| Magnetic Bead Suspension | – | – | – | – | – | 2 x 1.1 mL | 8.5 mL |
| Maxi Spin Columns | – | – | – | 12 | – | – | – |
| Mini Spin Columns | 50 | 100 | – | – | – | – | – |
| Midi Spin Columns | – | – | 20 | – | – | – | – |
| 96-Well Plate | – | – | – | – | 2 | – | 2 |
| Adhesive Tape | – | – | – | – | 4 | – | 2 |
| Collection Tubes | 50 | 100 | 20 | 12 | – | – | – |
| 96-Well Collection Plate | – | – | – | – | 2 | – | – |
| Elution Tubes | 50 | 100 | 20 | 12 | – | 50 | – |
| 96-Well Elution Plate | – | – | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
