Overview

• Fast and easy processing using rapid and convenient spin-columns
• Isolate high quality and high yield DNA
• DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping 

This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids.  The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping.  The protocol can be completed in as little as 1 hour.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity (gDNA)	10 μg
Maximum Loading Volume Per Spin Column	650 μL
Size of DNA Purified	All sizes > 80 bp
Maximum Amount of Starting Material	5 sections < 20 µm thick paraffin slices 25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.

Component	Cat. 47400 (50 preps)
Digestion Buffer A	25 mL
Buffer RL	30 mL
Wash Solution A	38 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
RNase A	1 tube
gDNA Purification Micro Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Safety Features

1. User-Friendly Design: The chamber lid and tabletop are covered with heat-insulating resin materials, providing both protection against burns and an aesthetically pleasing appearance. The upward-opening flip-top door saves space, while the rounded corners prevent injury. The specially designed steam discharge and collection system minimizes steam burn risks and enhances operational convenience.

2. Interlock Mechanism and Safety Protection: The safety interlock system is designed to be more sensitive and secure. The chamber lid cannot be opened if there is pressure or steam inside the chamber. The lid can only be opened when the pressure gauge reads “0” MPa. The sterilizer will not start heating if the chamber lid is not properly locked. These features enhance the overall safety of the machine.

3. Over-temperature and Heating Protection: The system continuously monitors the temperature inside the sterilization chamber. If the temperature becomes too high or heating is abnormal, power will be automatically cut off to prevent overheating.

4. Automatic Fault Detection System: The machine performs an automatic self-check when powered on. If a fault occurs during operation, an audible alarm will sound, and a fault code will be displayed.

5. Lid Closure Check System: The system automatically detects whether the chamber lid is securely locked. If the lid is not properly locked, the sterilizer will not start.

6. Leakage, Over-current, and Short-Circuit Protection: If there is leakage in the heating elements or other components, or if over-current or short-circuit conditions occur, the system will automatically cut off power to prevent user injury.

7. Water Shortage and Independent Dry-Burning Protection: Equipped with a low water level sensor and independent dry-burning protection device, the system can monitor the water level in the chamber in real-time to prevent dry burning and avoid false readings. If the low water level sensor fails, the overheat and dry-burning protection system will automatically activate, cut off power to the heating ring, and sound an alarm to ensure safety.

8. Dual Pressure Protection: The system includes both a mechanical safety valve and an electronic over-pressure protection system. If pressure becomes abnormal, the system will relieve pressure and cut off power while sounding an alarm.

9. Back end Safety Testing: The system can be periodically tested for safety and protective measures.

10. Over-pressure Testing: Regular testing ensures that the instrument’s over-pressure protection system can automatically cut off power or that the safety valve can open to relieve pressure.

11. Performance Testing: The instrument’s safety performance has been tested by national authorized inspection agencies, and a test report is available.

Technical Parameters

Model	HB65A Standard TypeHB65B  Auto Water Replenishment TypeHB65C Auto Drying Type	HB90AStandard TypeHB90BAuto Water Replenishment TypeHB90CAuto Drying Type	HB110AStandard TypeHB110BAuto Water Replenishment TypeHB110CAuto Drying Type
Capacity	65L	90L	110L
Dimensions (L × W × H) mm	664×660×994.5	664×660×994.5	664×660×1200
Sterilization Chamber Dimensions (Diameter × Height) mm	φ400×530	φ400×730	φ400×920
Rated Power	2800W	3400W	4500W
Rated Voltage	220V±10%50Hz
Temperature Display Accuracy	0.1℃
Operating Environment	5℃~40℃、Relative Humidity10%～85%
Sterilization Chamber Material	SUS304Stainless Steel
Sterilization Working Temperature	105℃~143℃
Sterilization Time Pre-set Range	1minute ～9999minutes
Melting Temperature Pre-set Range	60℃~100℃
Melting Time Pre-set Range	1minute～9999minutes
Insulation Temperature Pre-set Range	45℃~60℃
Insulation Time Pre-set Range	1minute～9999minutes
Door Opening Temperature Pre-set Range	Solid, Agar, and Waste Modes: 40°C ~ 99°C        Liquid Mode: 40°C ~ 80°C
Clock FunctionTimer Pre-set Range	Timer Pre-set Range: Set operating time (year, month, day, hour, minute), check current time (year, month, day, hour, minute), total sterilization cycles, fault occurrence time, and code.
Pressure Gauge Display Range	0～0.6Mpa
Pressure	Chamber Design Pressure: 0.35 MPa; Each product comes with a quality certificate and a certificate issued by the special equipment manufacturing supervision and inspection department.
Pressure Safety Protection	Dual independent pipeline measurement and control, mechanical and electronic dual pressure protection
Sterilization Modes	·  Solid Mode: Heating → Sterilization → Exhaust, Air Cooling·  Solid with Drying Mode: Heating → Sterilization → Drainage, Exhaust → Drying·  Liquid Mode: Heating → Sterilization → Pre-cooling → Exhaust·  Liquid with Insulation Mode: Heating → Sterilization → Pre-cooling → Exhaust → Insulation·  Waste Mode: Heating → Sterilization → Exhaust, Air Cooling·  Agar Mode: Heating → Melting → Insulation
Exhaust Control and Water Tank	Fully automatic internal exhaust (see instructions for exhaust temperature settings), built-in large-capacity steam condensate collection bottle and water tank
Cover Opening Method and Interlocking Device	Unique eight-column interlocking device, flip-cover switch, touch-type handle
Controller and Memory Storage Function	Microcomputer intelligent control system. 6 fixed operation modes, 82 modifiable modes, 88 memory storage programs
Safety Devices	Door lock interlocking device; Cover closing check; Dry burn protection; Water level sensor monitoring; Electronic over pressure protection; Mechanical safety valve; Over temperature and heating protection; Over current and short circuit protection; Leakage protection; Cooling lock; Burn injury safety protection; Automatic fault detection system
Accessories	Condensate collection bottle, water separation plate, drainage hose, stainless steel basket
Optional Accessories	Printer, cooling fan, item temperature sensor, USB output
Stainless Steel Baskets	(φ360×230)1pcs(φ360×200)1pcs	(φ360×200)1pcs(φ360×400)1pcs		(φ360×200)2pcs(φ360×400)1pcs

Note: The upper limits for cooling lock door temperature and exhaust temperature are related to the boiling point.

Other Optional Accessories

1. Dot Matrix Printer: Capable of printing temperature, time, F0 value, and pressure data. USB data export and curve plotting.

2. Stainless Steel Basket with Base.

3. Pressure Sensor

4. Stainless Steel Bucket

5. Item Thermometer

6. 3Q Validation Adapter

Features and functions:

1. Large-diameter sterilization chamber：The 40 cm large-diameter sterilization chamber provides an exceptionally spacious environment to meet a wide range of sterilization needs.

2. Sterilization temperature：105-138℃.

3. Exhaust control ：After sterilization is complete, the exhaust valve can automatically open to release the exhaust at a pre-set temperature.

4. Verification interface: Provides temperature and pressure inspection ports for easy calibration.

5. Saturated steam monitoring：The system automatically monitors the expulsion of cold air to ensure a pure steam sterilization environment, guaranteeing optimal sterilization effectiveness.

6. Pressure performance：The chamber is designed to withstand a pressure of up to 0.35 MPa, and each unit is tested with a water pressure of up to 0.44 MPa. Advanced pressure protection ensures the safety load of the equipment.

7. Intelligent control system:The microcomputer chip-based intelligent control system monitors the temperature and pressure inside the sterilization chamber in real-time. The chamber lid can only be opened under safe temperature and pressure conditions. The system also provides fully automatic control of the sterilization process and allows for convenient maintenance, inspection, calibration, recording, and future upgrades.

8. Six preset programs:Includes six fixed programs for liquid sterilization and holding, solid sterilization and drying, agar melting and holding, and waste sterilization.

9. Memory storage system:Users can set their own parameters, create multiple programs, and access them at any time. The system allows for operation permissions to be set at three levels—user, administrator, and engineer—to facilitate effective use and management of the sterilizer.

10. Door-opening temperature: Each program can be set with a specific cooling lock door-opening temperature based on the thermal inertia of the sterilized items. This prevents users from opening the door when the items (especially liquid sterilized items) are still in an unsafe condition, thus avoiding burns.

11. Drying function: Thorough drying that meets CE standards, with a cooling lock mechanism.

12. One-touch inquiry function: Allows checking of current time, total number of sterilization cycles, fault occurrence time, and codes.

13. Scheduled start function: Allows you to set a start time so that the sterilizer automatically starts at the scheduled time.

Model:
HB65/ HB90/ HB110

A reagent dispensing device which allows a full liter of constant reagent delivery

Permagen Reagent Trough Birdfeeder Kit with Bottle

The Permagen Reagent Trough Birdfeeder Cartridge and Bottle is a device to deliver 1 liter of reagent on the Biomek FX and NX Laboratory Automation Workstations. The unit fits on any standard ALP stand as well

 

• Extended version or Standard available, extended designed to be refilled outside liquid handler, modification to machine may be necessary 
• Self-leveling fluid line assures the trough will have adequate volume 
• Hooks onto a Beckman® ALP stand or deck
• ANSI/ SBS compliant footprint   
• Will stand up on bench to refill etc.
• Available in white or black trough
• Made in the USA
• Pull down menu to choose product. Available in Extended version (black or white), standard version in white, or valve only 

Please contact us for volume discounts, OEM opportunities, or private labeling.    

A reagent dispensing device which allows a full liter of constant reagent delivery