This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
| Features | Specifications |
| Main Functions | Extract viral DNA/RNA from200μl samples by magnetic beads |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral DNA / RNA, body cell DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technolog |
| Process method | Manual or automatic |
| Sample type | |
| Sample amount | 200μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Yield | |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
| Contents | IVD5412 |
| Purification Times | 200 Preps |
| MagPure Particles N | 5 ml |
| PK/Carrier RNA | 50 mg |
| Protease Dissolve Buffer Blue | 5 ml |
| Buffer MLB | 120 ml |
| Buffer MW1 | 53 ml |
| RNase Free Water | 30 ml |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Experiment Data
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic Gram-negative bacterium that could survive low temperatures and freezing. The majority of the 1.3 billion annual cases of Salmonella-caused human gastroenteritis result from ingestion of contaminated food products, such as raw or undercooked meat, seafood, and eggs, as well as raw or unpasteurized milk and dairy products. Salmonella infections are also contracted following consumption of fresh fruits or vegetables that have been contaminated by infected fertilizer.
Salmonella enterica TaqMan PCR Kit, 100 reactions
Salmonella enterica TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM32150 (100 preps) | Cat. TM32110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| S. enterica Primer & Probe Mix | 280 μL | 280 μL |
| S. enterica Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
RLE500
Maximum – 150 µL
Minimum – 5 µL
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation