K-FGLQR
SKU: 700007621
50 assays (manual) / 500 assays (auto-analyzer)
| Content: | 50 assays (manual) / 500 assays (auto-analyzer) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Analyte: | D-Fructose, D-Glucose |
| Assay Format: | Spectrophotometer, Auto-analyzer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 2.5 to 150 µg of D-glucose plus D-fructose per assay |
| Limit of Detection: | 6 mg/L |
| Limit of Quantification: | 16 mg/L |
| Reproducibility (%): | < 5% |
| Reaction Time (min): | ~ 15 min for Total Sugars |
The D-Fructose/D-Glucose (Liquid Ready™) assay kit is a method for the specific measurement and analysis of D-Fructose/D-Glucose in wine, beverages, foodstuffs and other materials. Measurements can be performed separately or combined. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
Find more of our monosaccharide and oligosaccharide assay kits.
Advantages
The D-Fructose/D-Glucose (Liquid Ready™) assay kit is a method for the specific measurement and analysis of D-Fructose/D-Glucose in wine, beverages, foodstuffs and other materials. Measurements can be performed separately or combined. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 minutes. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection, and in vitro translation.
Kit Contents
| Contents | R415002 | D415003 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 500 |
| TCEP (1M) | 0.29 g | 5 x 0.29 g |
| Buffer EP | 1.0 ml | 5.0 ml |
| Buffer PSL | 50 ml | 250 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.
2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.
4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.
This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 minutes. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection, and in vitro translation.
Human Cytomegalovirus (HCMV) or Human betaherpesvirus 5 (HHV-5) is a member of the Family Orthoherpesviridae. HCMV belongs to the betaherpesvirinae subfamily, while other herpesviruses fall into the subfamilies of Alphaherpesvirinae (including HSV 1 and 2 and varicella) or Gammaherpesvirinae (including Epstein-Barr virus). All herpes viruses share a characteristic ability to remain latent within the body over long periods of time. While CMV can be found in numerous body fluids including urine, saliva, breast milk, blood, tears, semen, and vaginal fluids, urine samples are generally used for congenital HCMV infection screening due to high viruria observed in infected infants.
CMV TaqMan PCR Kit, 100 reactions
CMV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM36350 (100 preps) | Cat. TM36310 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| CMV Primer & Probe Mix | 280 μL | 280 μL |
| CMV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |