This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from FFPE using high bind beads |
Applications | RT-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Large quantities of solids |
Sample amount | Appropriate |
Elution volume | ≥50μl |
Time per run | 30 – 120 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
1.Use non-toxic dewaxing solution without contact with xylene
2.Efficient removal of formaldehyde modification on DNA and improvement of PCR sensitivity
3. One of the best FFPE DNA extraction kits on the market, the same effect of paraffin slice extraction as top brand, and the effect of puncture sample extraction is even better than top brands
Contents | D632301B | D632302B |
Purification Times | 48 Preps | 96 Preps |
MagBind Particles | 1.1 ml | 2 x 1.1 ml |
RNase A | 10 mg | 20 mg |
Proteinase K | 24 mg | 48 mg |
Protease Dissolve Buffer | 3 ml | 6 ml |
Buffer DPS | 60 ml | 100 ml |
Buffer ATL | 15 ml | 30 ml |
Buffer AL | 15 ml | 30 ml |
Buffer BD* | 6 ml | 15 ml |
Buffer BXW1* | 13 ml | 44 ml |
Elution Buffer | 15 ml | 30 ml |
Storage and Stability
RNase A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.
Usages:
For cultivation of Escherichia coli.
Principle:
Peptone provide nitrogen, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; lactose into fermentable sugars.
Formulation(per liter):
Pancreatic Digest of Gelatin 20 g
Lactose Monohydrate 10g
Dehydrated Ox Bile 5g
Bromocresol Purple 10mg
final pH 7.3±0.2
How to use:
1.Suspend 35g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
Culture Plate 24 Wells
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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