Custom low volume, low profile/slim 384-well plates
Specially designed custom low volume, low profile/slim 384-well plates designed for use with our GeneArrayer. These plates are made from durable polypropylene delivering an excellent low fluorescence background interference and high performance when paired with our high-throughput genotyping instruments.
The unique, slimline plate design permits lower reaction volumes than standard 384-well plates (typically 1.6 – 2.0 µL) allowing for reaction miniaturization, with all the benefits this brings saving time, consumables and costs. This plate is designed specifically to be used with 3CR Bio’s GeneArrayer automated liquid handling instrument.
Plate Dimensions: Length 127.85 mm, Width 85.85 mm, Thickness 5.05 mm, Well Spacing 4.50 mm, Individual Well Capacity 3 µL.
Custom low volume, low profile/slim 384-well plates
Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Purification Technology
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 1.5 mg |
| Maximum Column Loading Volume | 20 mL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material | |
| Liver Tissue | 100 mg |
| Heart Tissue | 50 mg |
| Kidney Tissue | 100 mg |
| Brain Tissue | 250 mg |
| Lung Tissue | 100 mg |
| Whole Blood | 2 – 10 mL* |
| Bacteria | 2.5 x 10 10 cells |
| Yeast | 2 x 108 cells |
| Fungi | 1 g |
| Plant Tissues | 1 g |
| Time to Complete 4 Purifications | 40 minutes |
| Average Yield: HeLa Cells (5 x 107 cells) E. coli (2.5 x 1010 cells) | ~750 μg ~1.5 mg |
*For isolating total RNA from purified leukocytes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 26800 (8 preps) |
|---|---|
| Buffer RL | 2 x 40 mL |
| Wash Solution A | 4 x 38 mL |
| Elution Solution A | 3 x 20 mL |
| RNA Maxi Spin Columns (with collection tubes) | 8 |
| Elution Tubes (50 mL) | 8 |
| Product Insert | 1 |
