Description 

The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank. 

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months
• Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 3,000 bp 

Concentration 

56 µg/ 500 µl

Recommended loading volume 

5 µl/ well

Storage

Protected from light 
Room temperature for 6 months
4°C for 12 months 
-20°C for 24 months 

The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.

Introduction

Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:

1. The sample is highly infectious, posing great harm to operators and the environment.

2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;

3. Blood sample contains a large amount of impurities and inhibitory factors.

Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.

The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern Blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 2ml blood and 200mg tissue using Midi column
Applications	PCR, southern bolt and virus detection, etc
Purification method	Midi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount	0.2-2 ml
Elution volume	≥300μl
Time per run	≤80 minutes
Liquid carrying volume per column	4ml
Binding yield of column	1mg

Principles

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast – without separation of leukocytes, organic extraction or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Wide applicability – handle a variety of liquid samples

Kit Contents

Contents	D311302	D311303
Purification Times	20	100
HiPure gDNA Midi Columns	20	100
15ml Collection Tubes	40	200
Buffer ATL	50 ml	250 ml
Buffer AL	50 ml	250 ml
Buffer GW1*	22 ml	110 ml
Buffer GW2*	12 ml	50 ml
RNase A	20 mg	90 mg
Proteinase K	100 mg	440 mg
Protease Dissolve Buffer	10 ml	30 ml
Buffer AE	20 ml	120 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Sample amount	Leukocyte protocol*	Colum type	Elutio volume	Average yield	Time per run
HiPure Blood DNA Mini Kit	D3111	10-200μl	1ml	2ml column	≥20μl	5-9μg/200μl	≤30 minutes
HiPure Tissue&Blood DNA Midi Kit	D3113	0.2-2ml	10ml	1.5ml column	≥300μl	20-40μg/1m	≤80 minutes
HiPure Tissue&Blood DNA Maxi Kit	D3115	3 -10ml	10ml	15ml column	≥700μl	20-40μg/1m	≤90 minutes
HiPure Tissue&Blood DNA 96 Kit	D3117	1-200μl	1ml	96 well plate		3-8μg/200μl

Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:

TGL20 Technical Parameters
Max Speed	21000r/min	Max Volume	6×100ml
Timer	1~99h59min	Dimension	570×620×380mm
Speed Accuracy	±20r/min	Max RCF	30910×g
Noise	≤58dBA	Net Weight	84KG
Power supply	AC 220V, 50HZ 10A	Temperature Range	-20℃~40℃
Temperature Accuracy	±1℃

Matched Rotors for TGL20
Order no	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(*g)
G20-1	Fixed Rotor	16000	4×8PCR	15760
G20-2	Fixed Rotor	15000	6×8PCR	21420
G20-3	Fixed Rotor	16000	8×8PCR	17480
G20-4	Fixed Rotor	15000	12×8PCR	22930
G20-5	Fixed Rotor	21000	12×1.5/2ml	30910
G20-6	Fixed Rotor	15000	40×0.5ml	22920
G20-7	Fixed Rotor	17000	24×1.5/2ml	26460
G20-8	Fixed Rotor	13500	30×1.5/2ml	19340
G20-9	Fixed Rotor	13000	48×1.5/2ml	17930
G20-10	Fixed Rotor	16000	16×5ml	22020
G20-11	Fixed Rotor	16000	6×10ml	21500
G20-12	Fixed Rotor	15000	12×10ml	22680
G20-13	Fixed Rotor	16000	12×7ml	21380
G20-14	Fixed Rotor	13000	16×10ml	19490
G20-15	Fixed Rotor	10000	12×15ml	11840
G20-16	Fixed Rotor	13000	8×15ml(falcon)	17790
G20-17	Fixed Rotor	5000	24×15ml	3500
G20-18	Fixed Rotor	15000	8×20ml	22680
G20-19	Fixed Rotor	5000	30×15ml	3830
G20-20	Fixed Rotor	14000	6×30ml	19060
G20-21	Fixed Rotor	13000	6×50ml(falcon)	18840
G20-22	Fixed Rotor	13000	6×50ml(round)	18730
G20-23	Fixed Rotor	13000	4×85ml	18940
G20-24	Fixed Rotor	12000	6×70ml	15570
G20-25	Fixed Rotor	12000	4×100ml	14850
G20-26	Fixed Rotor	12000	6×100ml	16390
G20-27	Fixed Rotor	12000	24 pieces capillary vessel	15800
G20-28	Fixed Rotor	18000	30×0.5ml	26660
G20-29	Swing Rotor	13000	4×5ml	14960
G20-30	Vertical Rotor	16000	16×5ml	16540
G20-31	Vertical Rotor	14000	8×30ml	16450
G20-32	Microplate Rotor	4000	2×3×48（well）	2300

Features:
1. Digital display indicates the speed,time,temperature and RCF.
2. Brushless DC motor in great torque, free maintenance,no powder pollution.quick in speed up and down.
3. There are many kinds of rotors for choice.
4. Automatically electric lid lock,super speed,over temperature protection and imbalance protection.
5. The centrifuge body is made of high-quality steel,safe and reliable.