Bis-propargyl-PEG5 is a crosslinker containing propargyl groups at both ends. The propargyl groups reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form stable triazole linkages. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG5 is a crosslinker containing propargyl groups at both ends. The propargyl groups reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form stable triazole linkages. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters | Details |
---|---|
Product Name | DNA Isothermal Amplification Kit NFO |
Manufacturer | Amp-future |
Storage Temperature | -20°C |
Kit Components | Enzymes, Buffers ,Reagents |
Packaging | 48 Tests/box |
Detection Limit | 500-1000copies/µL |
Shipping | ICE |
Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
genesig 32 Overview
The genesig q32 Real-Time PCR Instrument is one of the fastest qPCR instruments on the market today due to its rapid heating and cooling. Assembled from just a few building blocks, this robust qPCR instrument allows analysis of up to 32 samples in both tube or strip format. The powerful genesig q32 software is easily installed from a USB flash drive onto any PC or Mac. The genesig q32 is used with 550 genesig Real-Time PCR Kits for human, vet, and food pathogens, as well as food speciation testing
The genesig Real-Time PCR Kit product range includes tests for a massive range of applications:
• Human Infectious Disease Screening
• Veterinary Diagnostics
• Food and Water testing
• Biothreat Detection
genesig q32 – Cutting edge design and technology
Assembled from just a few building blocks the robust genesig q32 system is easy to transport and install. Up to 32 samples can be run in 0.1 ml tubes or 8-strip format. Fast heating and cooling is achieved by utilizing robust Peltier elements, whilst assay performance is supported further by a heated lid design. Excitation is provided by high intensity LEDs filtered to provide light at 500nm that is capable of exciting all fluorophores commonly used in qPCR. A prism is used to generate spectra from fluorescent emissions. These spectra are imaged using a CMOS camera.
genesig q32 software
The genesig q32 software is delivered and easily installed from a USB flash drive. When installed in a network, the software can control several q32 instruments connected via LAN. The powerful and easy to use software provides the following features:
• Quick start using templates for all genesig kit applications
• Straightforward setup and editing of sample and target information
• Automated analysis modules for absolute and relative quantification, melting curve analysis and endpoint genotyping
• Analysis of full spectral data
• Compatible with Windows, Mac & Linux Systems
• Instrument start from a USB flash drive, using preprogrammed settings
• Data export functions
genesig Real-Time PCR Kit compatible
genesig Real-Time PCR Kits are suitable for use on the genesig q32 instrument. The genesig Kit range includes over 550 kits for human, vet, and food pathogens, as well as food speciation testing. All genesig Kits include a positive control and resuspension buffers. The kits come freeze-dried so that they can be shipped at room temperature for fast delivery to anywhere in the World.
Automated run templates available for:
• genesig Easy Real-Time PCR Kits
• genesig Advanced Real-Time PCR Kits
• genesig Standard Real-Time PCR Kits
• snpsig Real-Time PCR Kits
• quasa Real-Time PCR Kits
• GMO Real-Time PCR Kits
Guaranteed results using 550 genesig kits
Fast DNA and RNA analysis in less than 60 minutes
Quick and easy set-up and run
Automated data analysis result calling
Small and robust instrument
Exceptional value for money
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