Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp(Nucleic Acid). The HiPure Circulating Nucleic acid Micro Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be either fresh or frozen (provided that they have not been frozen and thawed more than once). Free-circulating cell-free DNA, RNA or viral nucleic acids are eluted in Nuclease Free Water, ready for use in amplification reactions or storage at -30 to -15°C. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Specifications
Features | Specifications |
Main Functions | Isolation circulating DNA from 0.6ml plasma,serum, body fluids |
Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Serum, plasma and other cell-free fluid samples |
Sample amount | 0.6ml |
Elution volume | ≥30μl |
Time per run | ≤40 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
This product is based onsilica column purification. The sample is lysed and digested with lysate andprotease, DNA is released into the lysate. Transfer to an adsorption plate andfilter column. Nucleic acid is adsorbed on the membrane, while protein is notadsorbed and is removed with filtration. After washing proteins and otherimpurities, Nucleic acid was finally eluted with low-salt buffer.
Kit Contents
Contents | D318002 | D318003 |
Purification Times | 50 Preps | 250 Preps |
Buffer ACL | 40 ml | 200 ml |
Buffer DCW1 | 22 ml | 110 ml |
Buffer DCW2* | 20 ml | 2 x 50 ml |
Proteinase K | 34 mg | 180 mg |
Protease Dissolve Buffer | 1.8 ml | 10 ml |
Carrier RNA | 110 μg | 310 μg |
Nuclease Free Water | 10 ml | 30 ml |
HiPure CFDNA Mini Columns | 50 | 250 |
2 ml Collection Tubes | 100 | 5 x 100 |
Storage and Stability
Proteinase K, Carrier RNAshould be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15-25°C) andare stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use.Make sure that all buffers are at room temperature when used.
Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,
Intended Use
For the selective separation and enumeration of enterococci in food and water.
Principle and Interpretation
Tryptone and peptone are the sources of nitrogen and essential growth factors. Yeast extract acts as well nitrogenous compounds and additionally the vitamin B12 complex. Sodium azide acts largely inhibits the growth of gram-negative bacteria while sparing enterococci, staphylococci and streptococci. Ox bile inhibits most gram positives but not enterococci. Enterococci hydrolyse esculin to esculetin and dextrose, which reacts with ferric citrate producing a brownish black precipitate around the colonies. Tolerance to bile and the ability to hydrolyze esculin is the traditional and reliable test for the identification of enterococci. (4). Sodium chloride maintains the osmotic balance of the medium and Agar is the solidifying agent.
Formulation
Ingredients | /liter |
Tryptone | 17.0g |
Ox bile | 10.0g |
Yeast extract | 5.0g |
Sodium chloride | 5.0g |
Peptone | 3.0g |
aesculin | 1.0g |
Ferric ammonium citrate | 0.5g |
Sodium azide | 0.15g |
Agar | 15.0g |
pH 7.1±0.1 at 25°C |
Preparation
Weigh 56.6g of dry powder of this product, add 1 L of distilled water or deionized water, stir, heat and boil until completely dissolved, and sterilize at 121℃ for 15 min.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 20-24hours.
Quality control strains | Growth | Colony color |
Enterococcus faecalis ATCC29212 | PR≥0.7 | Brown-black halo |
Escherichia coli ATCC25922 | inhibited | Absence of brown-black halo |
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the selective separation and enumeration of enterococci in food and water. Principle and Interpretation Tryptone and peptone are the sources of nitrogen and essential grow……
Blood Urea Nitrogen Enzymatic Kit is a microplate-based colorimetric
assay for the determination of urea in serum samples produced from blood. Blood urea nitrogen (BUN) is an important marker for normal kidney and liver function. Elevation of BUN levels is often an indication of intestinal and kidney obstruction and cardiac failure. Decreased BUN levels are often associated with kidney and liver damage. BUN is also a very useful tool
for preclinical investigation of experimental drug formulations and BUN levels are commonly used to monitor and attenuate the toxic effects of experimental drug formulations in rodents.
Blood Urea Nitrogen Enzymatic Kit uses an enzyme-based assay to determine urea in liquid samples such as serum. The test is based on a highly proven method for urea determination. The Blood Urea Nitrogen Enzymatic Kit contains sufficient materials to test 42 samples in duplicate. The assay utilizes urease, a metabolic enzyme, to specifically detect urea in serum. The Blood Urea Nitrogen Enzymatic Kit provides rapid, accurate, proven results even in complex liquid mixtures. The limit of detection for the test is 8 ppm urea for serum. The linear range of the assay is 8 – 200 ppm analyte.
96 determinations
Rapid and simple method
Minimal sample prep
Highly accurate and reproducible
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