64×10ml Swing Buckets Rotor

12 x 8 strips (96 tests)

Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.

Name of Product

Taenia solium – IgG ELISA

Catalog Number

AF 9700

Short Info

Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

98% sensitivity, 98% specificity

Test Principle

The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Taenia solium cyst soluble antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Taenia solium specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader. The test can be performed with automatic systems, but this must be validated by the user.

Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.

Description 

The DM2100 ExcelBand™ 100 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2100 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. 

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. 

Range 

100 ~ 1,500 bp 

Concentration 

52.2 µg/ 500 µl 

Recommended loading volume 

5 µl/ well 

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months

The DM2100 ExcelBand™ 100 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2100 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. 

DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.