This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).
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| Kit Specifications | |
| Column Binding Capacity | 25 µg |
| Average Yield:* HeLa Cells (1 x 106) Tissue (from 10 mg kidney) | 8 µg 10 µg |
| Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension | 20 mg 3 x 106 cells 150 µL 150 µL |
| Time to Complete 10 Purifications | 80 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 24700 (50 preps) | Cat. 24750 (100 preps) | Cat. 24770 (250 preps) |
|---|---|---|---|
| Digestion Buffer A | 25 mL | 2 x 25 mL | 5 x 25 mL |
| Buffer SK | 30 mL | 2 x 30 mL | 5 x 30 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 5 x 18 mL |
| Elution Buffer B | 30 mL | 2 x 30 mL | 5 x 30 mL |
| Proteinase K | 12 mg | 2 x 12 mg | 5 x 12 mg |
| Spin Columns | 50 | 100 | 250 |
| Collection Tubes | 50 | 100 | 250 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 |
| Product Insert | 1 | 1 | 1 |
R-CAAR4
SKU: 700005034
200 assays per kit (4 vials)
| Content: | 200 assays per kit (4 vials) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Limit of Detection: | 0.05 U/mL |
| Reproducibility (%): | ~ 3% |
| Reaction Time (min): | ~ 30 min |
Purchase high purity Ceralpha: α-Amylase Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
p-Nitrophenyl α-D-maltoheptaoside (blocked), plus excess α-glucosidase and glucoamylase. For the measurement of cereal, fungal and bacterial α-amylase.
View our full range of reagent mixtures.
Purchase high purity Ceralpha: α-Amylase Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
p-Nitrophenyl α-D-maltoheptaoside (blocked), plus excess α-glucosidase and glucoamylase. For the measurement of cereal, fungal and bacterial α-amylase.
View our full range of reagent mixtures.
