Attogene’s Carbon Dioxide Enzymatic Assay Kit is a simple, direct method for measuring Carbon Dioxide levels in the environment. The assay uses a coupled enzyme assay to detect CO2 (as HCO3-) as follows. In the first step, the bicarbonate condenses with phosphoenol pyruvate to form oxalate (and phosphoric acid); this reaction is catalyzed by the enzyme Phosphoenolpyruvate Decarboxylase, PEPC. The oxalate is then enzymatically reduced by the enzyme Malate Dehydrogenase (using an NADH cofactor) to form malate and NAD+.
Norgen’s Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen’s Saliva DNA Preservative contained within a sealed squeezable ampoule. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen’s Saliva DNA Isolation Kit (Cat# Dx45400). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for in vitro diagnostic use for medical purposes.
Norgen’s Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.
Prior to saliva DNA isolation, vortex the Collection Tube containing preserved saliva for 10 seconds and incubate at 55°C for one hour. Saliva DNA can now be isolated from the preserved saliva samples using Norgen’s spin-column based Saliva DNA Isolation Kit Dx (Cat# Dx45400) or other commercially available methods that have been validated for use with Norgen’s Saliva DNA Collection and Preservation Devices.
NOTE: This product is not available for sale in the United States.
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| Kit Specifications | |
| Volume of Saliva Collected | 2 mL |
| Volume of Saliva-Preservative Mix | 4 mL |
| Preservation Temperature | Room Temperature |
| Preservation time | Over 2 years at room temperature |
Shelf Life and Handling
| Kit Components | Cat. 49000 (50 Devices) |
|---|---|
| Individual Saliva DNA Collection and Preservation Devices | 50 |
| Donor Procedure Flowchart | 1 |
| Product Insert | 1 |
| Individual Saliva DNA Collection and Preservation Device Contents | |
| Saliva Collection Funnel and Collection Tube | 1 |
| Collection Tube Cap | 1 |
| Preservative Ampoule | 1 |
| Donor Instructions | 1 |
For the rapid detection and enumeration of coliform bacteria.
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine flora β- galactosidase-glucosidase specific reaction, hydrolysis of the substrate, the release of the color groups produce green colonies on the light yellow plate.
Formulation (per liter):
Peptone :10g
Yeast extract powder: 3g
sodium chloride:5g
sodium lauryl sulfate:0.1g
Agar :12g
Chromogenic substrate 2.7g
Final pH 7.0 ± 0.2
How to use:
1. Weigh 32.8g of this product, adding 1L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing into flask without autoclaving.
2,Take 25g or 25mL of sample with aseptic procedures, added to the flask containing 225mL of sterile phosphate buffered saline (or saline) is shaken thoroughly homogenized with a homogenizer or a 1:10 dilution 1min, then 1:10 dilution continue to select the appropriate serial dilutions of three, two plates each dilution was inoculated.
3, the use of pour method: The medium is cooled in a water bath to about 50 , sterilized petri dish having a diameter 90mm, 1ml samples were inoculated per dish, then poured into about 15ml of the above dissolution medium, and mix to solidify, upside down, 37 for 24 hours.
4, using surface inoculation: cooled to about 50 , shake devoted to the medium in sterile petri dish. Can be stored in the refrigerator for a day or stored at room temperature for several days (dark, 4 ). The sample was streaked method or filter method, 37 for 24 hours.
5, observe the results.
Quality control:
This product appear light yellow after pouring into plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.
Growth of bacteria were cultured bacteria numbers feature
Escherichia coli ATCC25922 good green colonies
Citrobacter ATCC8090 good green colonies
Salmonella typhimurium CMCC50115 good colorless colonies
Enterococcus faecalis ATCC29212 suppressed —–
Storage: Store at 10-30 , dark, cool and dry place, tighten the cap immediately after use. Storage period of two years.
1000mL