Introduction

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Mini Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be fresh or frozen (provided that they have not been frozen and thawed more than once).

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 1ml plasma, serum, body fluids
Applications	qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Serum, plasma and other cell-free fluid samples
Sample amount	1ml
Elution volume	≥30μl
Time per run	≤50 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).

Advantages

• High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration – low elution volume, ensuring high nucleic acid concentration
• High purity – low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery – DNA can be recovered at the level of PG by silica gel column purification

Kit Contents

Contents	D318102	D318103
Purification Times	50 Preps	250 Preps
Buffer ACL	50 ml	250 ml
Buffer ACB*	60 ml	300 ml
Buffer DCW1*	22 ml	88 ml
Buffer DCW2*	10 ml	50 ml
Proteinase K	120 mg	540 mg
Protease Dissolve Buffer	10 ml	30 ml
Carrier RNA	110 μg	310 μg
Nuclease Free Water	10 ml	30 ml
HiPure CFDNA Mini Columns	50	250
2 ml Collection Tubes	100	500

Storage and Stability

Proteinase K and carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage(up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers shouldbe redissolved before use. Make sure that all buffers are at room temperature when used.

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,

Name of Product

HybriDetect 2T

Catalog Number

MGHD2 1

Short Info

The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products

Please note, that you may have to pay country-specific taxes and duties.

Method/Platform

Lateral flow, sandwich or competetive

Range/Assay Sensivity

5 pg DNA, equivalent to agarose gel electrophoresis

Test Principle

HybriDetect 2T is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for simultaneous detection of two different analytes such as proteins, antibodies or gene amplification products. The results can be interpreted qualitative or quantitative.

The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products

K-MBGL

SKU: 700004320

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 1 year under recommended storage conditions

Analyte:	endo-Cellulase, β-Glucanase/Lichenase
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	590
Signal Response:	Increase
Limit of Detection:	100 U/kg of malt
Reproducibility (%):	~ 7%
Reaction Time (min):	~ 35 min
Application examples:	Malt extracts, wort, beer and other materials.
Method recognition:	RACI Standard Method

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.

Looking for other assay kits? See our full range of enzyme assay kits.

Advantages

• Very cost effective 
• All reagents stable for > 2 years during use 
• Only kit available 
• Very specific 
• Simple format 
• Standard included

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.